Comparison of the membrane composition of Spiroplasma citri and the corn stunt Spiroplasma

Mudd, J.B.; Lee, I M; Liu, H Y; Calavan, E. C.

doi:10.1128/jb.137.2.1056-1058.1979

Cited by 7 publications

(4 citation statements)

References 11 publications

(11 reference statements)

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“…The higher incorporation of palmitate as compared with oleate noticed in ouir study was reported previously for S. citri (8,12,13). Moreover, we have demonstrated that in S. floricola, the major de novo synthesized phospholipid, PG, shows an unusual distribution of fatty acids.…”

Section: Spiroplasma Membrane Lipidssupporting

confidence: 81%

“…BC3 to S. citri (Tables 1 and 2) is in line with the marked genetic relatedness (more than 60% DNA homology) of these two organisms (3). Similarly, the lipid composition of S. citri was found to be almost identical to that of the corn-stunt spiroplasma (13,14), an organism showing ca. 50% DNA homology with S. citri (3).…”

Section: Spiroplasma Membrane Lipidssupporting

confidence: 70%

“…Synthesis of PG as a major membrane lipid appears to be a property shared by all the spiroplasmas included in our study and by other mollicutes (15,19). The ability of spiroplasmas to incorporate exogenous phospholipids, combined with their capacity to take up substantial amounts of free and esterified cholesterol from the growth medium (8,12,13,16), relates the spiroplasmas to the sterol-requiring mycoplasmas, rather than to the acholeplasmas.…”

Section: Spiroplasma Membrane Lipidsmentioning

confidence: 66%

“…The spiroplasma membrane, like that of other mollicutes, is built essentially of protein and lipid (16). Data on membrane lipid composition is available for only the citrus pathogen Spiroplasma citri and for the genetically related corn-stunt spiroplasma (8,(12)(13)(14)16). The discovery of numerous new spiroplasmas which constitute an enormous group of microorganisms exhibiting diverse biological, pathological, and ecological properties (4,5,24,25) has prompted us to carry out a comparative analysis of membrane lipids in several Spiroplasma species and strains representing different serogroups.…”

mentioning

confidence: 99%

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Spiroplasma membrane lipids

Davis¹,

Katznel²,

Razin³

et al. 1985

J Bacteriol

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Membranes of six spiroplasma strains belonging to different Spiroplasma species and subgroups were isolated by a combination of osmotic lysis and sonication in the presence of EDTA to block endogenous phospholipase activity. Analysis of membrane lipids showed that in addition to free and esterified cholesterol the spiroplasmas incorporated exogenous phospholipids from the growth medium. Sphingomyelin was preferentially incorporated from phosphatidylcholine-sphingomyelin vesicles or from the serum used to supplement the growth medium. Palmitate was incorporated better than oleate into membrane lipids synthesized by the organisms during growth. The major phospholipid synthesized by the spiroplasmas was phosphatidylglycerol. The positional distribution of the fatty acids in phosphatidylglycerol of Spiroplasmafloricola resembled that found in Mycoplasma species, in which the saturated fatty acids prefer position 2 in the glycerol backbone and not position 1 as found in Acholeplasma species and elsewhere in nature. Electron paramagnetic resonance analysis of spin-labeled fatty acids incorporated into S. floricola membranes exhibited homogeneous single-component spectra without immobilized regions. The S. floricola membranes were more rigid than those of Acholeplasma laidlawii and less rigid than those of Mycoplasma gallisepticum.

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Section: Spiroplasma Membrane Lipidssupporting

confidence: 81%