Comparison of the Effect of Adipocyte-derived Stem Cells and Curcumin Nanoliposomes with Phenytoin on Open Cutaneous Wound Healing in Rats

Abstract

Background: Reducing the healing time of wounds can decrease the patient`s immobility time and their medical costs,leading a faster return of the patients to daily work. Objective: To compare the effect of adipose-derived stem cells and curcumin-containing liposomal nanoparticles with phenytoin on wound healing. Method: After anesthesia of the rats, open skin ulcers were made by a bistoury blade.Subsequently,stem cells were re-moved from the adipose tissue of theupper border of the epididymis. Then,the originality of stem cells was confirmed by the flow cytometry. The fusion method was used to prepare the liposome;and also nanoliposomal particles wereconfirmedby using the DLS microscope.The percentage of recovery and the cell count was measured with IMAGEJ.The expression of genes was assessed by PCR. The number of fibro blasts was counted by immuno histo chemistry techniques.The amount of collagen was determined by Tri-chromosome staining and the number of capillaries was enumerated byH & E staining. Results: The expression of TGF-β1 gene, vascular number, wound healing rate and the numberof fibroblasts increased significantly in adipose tissue-derived stem cells and curcumin nanoliposome groups(p<0.05);the wound surface was also decreased significantly(p<0.05). Conclusion: Based on the results of our research, adipose tissue-derived stem cells and curcumin nanoliposomescan heal wounds efficiently.

Phosphatidylserine accelerates wound healing and reduces necrosis in the rats: Growth factor activation

“…Eighteen rats were anaesthetised using 90 mg/kg intraperitoneal ketamine and 9 mg/kg intraperitoneal xylazine. The dorsal skin of the animals was shaved and two 2 × 2 cm full‐thickness excision wounds were created on the dorsum of each rat by cutting the skin layer 15 . They were divided randomly into the following three groups (n = 6); the rats of the first group were treated with 400 μL of topical PS hydrogel (PS group) on each rat, the second group was treated with 400 μL of topical PHT (PHT group) daily until 14 days, and the third group received no treatment (control (C) group).…”

“…The dorsal skin of the animals was shaved and two 2 Â 2 cm full-thickness excision wounds were created on the dorsum of each rat by cutting the skin layer. 15 They were divided randomly into the following three groups (n = 6); the rats of the first group were treated with 400 μL of topical PS hydrogel (PS group) on each rat, the second group was treated with 400 μL of topical PHT (PHT group) daily until 14 days, and the third group received no treatment (control (C) group). To assess macroscopic changes, photographs were taken of the wounds on days 0, 4, 7, 10 and 14 and the area of each wound was measured using Image J software.…”

Curcumin‐based nanoformulations alleviate wounds and related disorders: A comprehensive review

“…Taken together, they expressed liposomes as beneficial carriers, and penetration enhancers can enhance drug accumulation and inhibit skin lesion formation in the skin. 149 The Ebrahimzade et al's study 150 aimed to contribute to the growing area of research by finding a promising wound-healing method. They prescribed adipose-derived stem cells apart from CUR containing liposomal NPs with phenytoin to rats with open skin ulcers and compared their wound healing efficacy.…”

“…The Ebrahimzade et al's study 150 aimed to contribute to the growing area of research by finding a promising wound‐healing method. They prescribed adipose‐derived stem cells apart from CUR containing liposomal NPs with phenytoin to rats with open skin ulcers and compared their wound healing efficacy.…”