Comparison of the Binding of [<sup>3</sup>H]Spiperone and [<sup>3</sup>H]Domperidone in Homogenates of Mammalian Retina and Caudate Nucleus

Watling, Keith J.; Iversen, Leslie L.

doi:10.1111/j.1471-4159.1981.tb04663.x

Cited by 50 publications

(13 citation statements)

References 41 publications

(46 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This conclusion is supported by the detection of dopamine in retinal neurons by the Falk-Hillarp method (Ehinger, 1966), the demonstration of dopamine synthesis and the detection of light-evoked release of dopamine in the retina (Da Prada, 1977;Kramer, 1971), and the presence of dopamine-specific receptors (Watling & Iversen, 1981). This conclusion is supported by the detection of dopamine in retinal neurons by the Falk-Hillarp method (Ehinger, 1966), the demonstration of dopamine synthesis and the detection of light-evoked release of dopamine in the retina (Da Prada, 1977;Kramer, 1971), and the presence of dopamine-specific receptors (Watling & Iversen, 1981).…”

Section: Catecholamines In the Vertebrate Retinamentioning

confidence: 93%

Presumptive catecholaminergic ganglion cells in the pigeon retina

et al. 1990

View full text Add to dashboard Cite

An antiserum directed against tyrosine hydroxylase (TH), the rate-limiting enzyme in the synthesis of dopamine, was used to study the pigeon retina. Labeled cells were observed in both the inner nuclear layer (INL) and ganglion cell layer (GCL). Two populations of TH-immunoreactive neurons were observed in the INL. Some of these cells were 7-10 /im in diameter and gave rise to processes that arborized in three layers of the inner plexiform layer (IPL). These cells appeared similar to the dopaminergic amacrine cells described previously (Marc, 1988). Other labeled cells in the INL were 12-20 ^m in diameter and were recognizable as a previously described subpopulation of TH-immunoreactive displaced ganglion cells (Britto et al., 1988).A population of labeled cells was observed in the GCL. Counts of these cells in two retinae revealed 5000 and 7000 cells, respectively. They ranged in size from 8-15 pm in diameter in the central retina and from 8-20 pm in diameter in the peripheral retina. The density of labeled cells was highest in the central retina and red field and lowest in the retinal periphery. The difference in cell size and cell density as a function of eccentricity is characteristic of the total population of ganglion cells in the avian retina (Ehrlich, 1981;Hayes, 1982). Some of the TH-positive cells in the GCL could be classified as ganglion cells for two reasons: (1) The axons of many of the TH-positive cells in the GCL were TH-immunoreactive as well and could be followed to the optic nerve head. (2) The injection of rhodamine-Iabeled microspheres into the nucleus geniculatus lateralis, pars ventralis (GLv), resulted in the retrograde labeling of many of the TH-positive cells in the contralateral retina.

show abstract

Section: Catecholamines In the Vertebrate Retinamentioning

confidence: 93%

Presumptive catecholaminergic ganglion cells in the pigeon retina

et al. 1990

View full text Add to dashboard Cite

show abstract

“…was chosen to investigate putative hypothalamic and pitu¬ itary gland dopamine receptors in mammalian neural tissue (Baudry, Martres & Schwartz, 1979;Sokoloff, Martres & Schwartz, 1980;Watling & Iversen, 1981). …”

Section: Measurement Of Dopamine-binding Sitesmentioning

confidence: 99%

The functional activity of hypothalamic dopamine in broody bantam hens

Macnamee¹,

Sharp²

1989

Journal of Endocrinology

View full text Add to dashboard Cite

An assessment was made of the possible role of hypothalamic dopamine in the regulation of changes in plasma prolactin and LH in laying and broody bantam hens. Specific dopamine-binding sites were identified, using [3H]domperidone, in the anterior pituitary gland and in the anterior and posterior hypothalamus. The mean concentrations of dopamine-binding sites in both parts of the hypothalamus were 59-66 fmol/mg protein and did not differ between laying and incubating hens. The concentration of dopamine binding sites in the anterior pituitary gland was significantly (P less than 0.001) greater in laying than in incubating hens (278 +/- 46 compared with 420 +/- 32 fmol/mg protein, n = 5). The turnover rates of dopamine were compared in the anterior and posterior hypothalami of laying, incubating and nest-deprived hens. The turnover rates were estimated from the rate of accumulation of dopamine after inhibiting its catabolism using the monoamine oxidase inhibitor, pargyline, or by measuring the ratio of the concentrations of dopamine and its major metabolite, homovanillic acid. Both methods gave the same results. The turnover of dopamine was increased in the anterior but not posterior hypothalamus of incubating hens when compared with laying or nest-deprived hens. These results show, for the first time in birds, that the anterior pituitary gland contains specific binding sites for dopamine and that the concentration of these binding sites is inversely related to the concentration of plasma prolactin. The marked increase in dopaminergic activity in the anterior hypothalamus of incubating hens may stimulate the release of unidentified prolactin-releasing factors and/or inhibit the release of LH by exerting an inhibitory influence in the area of the hypothalamus containing LHRH cell bodies.

show abstract

“…A highly specific dopamine-sensitive adenylate cyclase is found in the teleost retina that possesses pharmacological properties very similar to those of the dopamine-sensitive adenylate cyclase in the mammalian brain (12,13). Although most dopamine receptors in the retina appear to be of the DI type (14,15), recent evidence suggests the presence of a small population ofdopamine receptors not associated with adenylate cyclase (16).…”

mentioning

confidence: 95%

“…The remaining 16 ml of Ringer's solution, containing clumps of cells, was discarded. The gradient vessels were funnel-shaped, with a diameter of 5 cm and a depth of 7 cm.…”

mentioning

confidence: 99%

Isolated horizontal cells from carp retina demonstrate dopamine-dependent accumulation of cyclic AMP.

Buskirk¹,

Dowling²

1981

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Horizontal cells of the carp retina were separated from other retinal cell types by using enzymatic dissociation and velocity sedimentation at unit gravity. Fractions containing horizontal cells were tested for their ability to accumulate cyclic AMP in the presence of various putative neurotransmitters. Micromolar concentrations ofdopamine, when added in the presence of 3-isobutyl-1-methylxanthine, stimulated cyclic AMP accumulation in these isolated cells. The dopamine-dependent accumulation of cyclic AMP in intact isolated horizontal cells was blocked by nanomolar concentrations of dopamine antagonists such as haloperidol, (+)-butaclamol, and fluphenazine. The results indicate that there is a postsynaptic dopamine receptor on carp horizontal cells that is associated with adenylate cyclase [ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1].

show abstract

Comparison of the Binding of [³H]Spiperone and [³H]Domperidone in Homogenates of Mammalian Retina and Caudate Nucleus

Cited by 50 publications

References 41 publications

Presumptive catecholaminergic ganglion cells in the pigeon retina

Presumptive catecholaminergic ganglion cells in the pigeon retina

The functional activity of hypothalamic dopamine in broody bantam hens

Isolated horizontal cells from carp retina demonstrate dopamine-dependent accumulation of cyclic AMP.

Contact Info

Product

Resources

About

Comparison of the Binding of [3H]Spiperone and [3H]Domperidone in Homogenates of Mammalian Retina and Caudate Nucleus

Cited by 50 publications

References 41 publications

Presumptive catecholaminergic ganglion cells in the pigeon retina

Presumptive catecholaminergic ganglion cells in the pigeon retina

The functional activity of hypothalamic dopamine in broody bantam hens

Isolated horizontal cells from carp retina demonstrate dopamine-dependent accumulation of cyclic AMP.

Contact Info

Product

Resources

About

Comparison of the Binding of [³H]Spiperone and [³H]Domperidone in Homogenates of Mammalian Retina and Caudate Nucleus