Presumptive catecholaminergic ganglion cells in the pigeon retina

Abstract

An antiserum directed against tyrosine hydroxylase (TH), the rate-limiting enzyme in the synthesis of dopamine, was used to study the pigeon retina. Labeled cells were observed in both the inner nuclear layer (INL) and ganglion cell layer (GCL). Two populations of TH-immunoreactive neurons were observed in the INL. Some of these cells were 7-10 /im in diameter and gave rise to processes that arborized in three layers of the inner plexiform layer (IPL). These cells appeared similar to the dopaminergic amacrine cells described previously (Marc, 1988). Other labeled cells in the INL were 12-20 ^m in diameter and were recognizable as a previously described subpopulation of TH-immunoreactive displaced ganglion cells (Britto et al., 1988).A population of labeled cells was observed in the GCL. Counts of these cells in two retinae revealed 5000 and 7000 cells, respectively. They ranged in size from 8-15 pm in diameter in the central retina and from 8-20 pm in diameter in the peripheral retina. The density of labeled cells was highest in the central retina and red field and lowest in the retinal periphery. The difference in cell size and cell density as a function of eccentricity is characteristic of the total population of ganglion cells in the avian retina (Ehrlich, 1981;Hayes, 1982). Some of the TH-positive cells in the GCL could be classified as ganglion cells for two reasons: (1) The axons of many of the TH-positive cells in the GCL were TH-immunoreactive as well and could be followed to the optic nerve head. (2) The injection of rhodamine-Iabeled microspheres into the nucleus geniculatus lateralis, pars ventralis (GLv), resulted in the retrograde labeling of many of the TH-positive cells in the contralateral retina.

Laminar distribution and sources of catecholaminergic input to the optic tectum of the pigeon (Columba livia)

“…However, none of these regions contained double-labeled neurons, although cells containing the different markers were frequently found intermingled with one another in these zones. scribed by Keyser et al (1990). Although a small number of retinal ganglion cells are double-labeled following TeO injections and TH immunohistochemistry (Britto et al, 19891, and despite the occasional TH-immunoreactive fibers observed in layer 1, we could not detect any changes in the pattern of TH staining following either restricted scratch lesions or total retinal ablations.…”

Survey on Amacrine Cells Coupling to Retrograde-Identified Ganglion Cells in the Mouse Retina

“…A more reliable way to distinguish GCs from ACs, however, is retrograde labeling of GCs. [35][36][37] In the retrogradely labeled mouse retina, the specificity of GABA, glycine, ChAT, and nNOS antibodies in labeling retinal ACs has been recently confirmed, 28 but similar investigations have not been done for TH, 38 CR, [32][33][34] and PV. [29][30][31] Of the multiple subtypes of ACs, GABA ACs are the only subtype that have been confirmed to couple with GCs.…”

Dopaminergic Interplexif orm Cells in the Retina of Pigmented and Hypopigmented Quails (Coturnix coturnix japonica)

“…Keyser ct al. [20] report the presence of an additional population of presumptive THI ganglion cells in the gan glion cell layer (GCL). We demonstrate here that in quails, T H IR is observed in conven tional amacrine cells and IPCs both located in the amacrine cell sublayer of the INL.…”