Comparison of the Accu‐Chek Aviva Point‐of‐Care Glucometer with Blood Gas and Laboratory Methods of Analysis of Glucose Measurement in Equine Emergency Patients

Abstract

Background: More information is needed regarding accuracy of commonly used methods of glucose measurement in the critically ill horse.Hypothesis: Glucometry will have good agreement with a laboratory standard. Glucometry with plasma will have better agreement than when performed with whole blood.Animals: Fifty sequentially admitted equine emergency patients, aged 41year. Methods: Venous blood was collected at admission and immediately analyzed by point-of-care glucometry on both whole blood (POC/WB) and plasma (POC/PL), a multielectrode blood gas analyzer with whole blood (BLG), and a standard laboratory method with plasma (CHEM). Paired data were compared using Lin's concordance correlation, Pearson's correlation, and robust regression. Bias and limits of agreement were tested by the Bland-Altman technique. Bivariate regression analysis was used to explore confounding factors.Results: Concordance was significant for all comparisons, and was strongest for CHEM-POC/PL (0.977) and weakest for POC/WB-POC/PL (0.668). Pearson's correlation was excellent for all comparisons except those with POC/WB. All comparisons had excellent robust regression coefficients except those with POC/WB.Conclusions and Clinical Importance: POC glucometry with plasma had excellent agreement with a laboratory standard, as did blood gas analysis. POC glucometry with whole blood correlated poorly with a laboratory standard. These differences may be clinically important, and could affect decisions based on glucose concentrations.

Evaluation of a Veterinary Glucometer for Use in Horses

“…7 In adult horses admitted on emergency basis, whole blood glucose measurements by a point-of-care glucometer designed for human use were poorly correlated with the laboratory standard. 11 However, when plasma was separated and tested with the glucometer there was good agreement. 11 Improvement of accuracy with equine plasma glucose measurement with e a glucometer designed for human whole blood is likely due in part to the difference in distribution of glucose between plasma and erythrocytes in humans and horses.…”

“…11 However, when plasma was separated and tested with the glucometer there was good agreement. 11 Improvement of accuracy with equine plasma glucose measurement with e a glucometer designed for human whole blood is likely due in part to the difference in distribution of glucose between plasma and erythrocytes in humans and horses. Separation of plasma before testing introduces additional time and error into analysis, counteracting advantages of point-of-care technology.…”

“…9 Veterinary glucometers tailored to each species potentially are more accurate and require less preparation time than human glucometer counterparts. 11 The veterinary glucometer evaluated requires a small volume of whole blood for analysis and gives a rapid result. The objective of this study was to compare glucose measurements from a veterinary point-of-care glucometer b with a reference plasma chemistry analyzer.…”

Evaluation of neonatal vitality and blood glucose, lactate and cortisol concentrations in foals of the Paint Horse breed

“…Significant differences were detected Hollis et al 2008). In this study, the following laboratory values were adopted: blood glucose (96 -176 mg/dL) (Paradis 2006), and lactate (first 24 hours: 3±0.04 to 4.9±1.02mmol/L) (Axon & Palmer 2008).…”

Early and late analysis of postpartum stress in newborn foals

“…Blood glucose and lactate concentrations were assessed using portable analyzers and a drop of total blood for each test (Accu-Chek® Advantage, Roche Diagnostics, Brazil, and Accutrend® Plus, Roche, Mannheim, Germany, for blood glucose and lactate concentrations, respectively) (Tennent-Brown et al, 2007;Hollis et al, 2008).…”