Comparison of techniques for the extraction of the hypotensive drugs geniposidic acid and geniposide from Eucommia ulmoides

Wu, Shou-Yuan; Jiang, Xian; Chen, Qian; Chen, X. Q.

doi:10.1007/bf03245968

Cited by 14 publications

(7 citation statements)

References 25 publications

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“…At present, there is a renewed interest in developing new processes based on the use of different variables like temperature, solvent modifiers, and material ratio for the extraction of low molecular weight components that may be environmentally friendly and benign. Previous research documentation authenticates that temperature-assisted, enzyme-assisted, supercritical-fluid-based, and semibionic-based extractions are superior over conventional soxhlet-mediated extraction [ 14 ]. It is quite complex to predict the suitable experimental conditions for a given separation task, and therefore good experimental design becomes significantly important.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Extraction Optimization and Analysis of Flavonoids from the Flowers of Tabernaemontana heyneana by High Performance Liquid Chromatography Coupled to Diode Array Detector and Electron Spray Ionization/Mass Spectrometry

Sathishkumar¹,

Baskar²,

Aravind³

et al. 2013

ISRN Biotechnology

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Flavonoids are exploited as antioxidants, antimicrobial, antithrombogenic, antiviral, and antihypercholesterolemic agents. Normally, conventional extraction techniques like soxhlet or shake flask methods provide low yield of flavonoids with structural loss, and thereby, these techniques may be considered as inefficient. In this regard, an attempt was made to optimize the flavonoid extraction using orthogonal design of experiment and subsequent structural elucidation by high-performance liquid chromatography-diode array detector-electron spray ionization/mass spectrometry (HPLC-DAD-ESI/MS) techniques. The shake flask method of flavonoid extraction was observed to provide a yield of 1.2 ± 0.13 (mg/g tissue). With the two different solvents, namely, ethanol and ethyl acetate, tried for the extraction optimization of flavonoid, ethanol (80.1 mg/g tissue) has been proved better than ethyl acetate (20.5 mg/g tissue). The optimal conditions of the extraction of flavonoid were found to be 85°C, 3 hours with a material ratio of 1 : 20, 75% ethanol, and 1 cycle of extraction. About seven different phenolics like robinin, quercetin, rutin, sinapoyl-hexoside, dicaffeic acid, and two unknown compounds were identified for the first time in the flowers of T. heyneana. The study has also concluded that L16 orthogonal design of experiment is an effective method for the extraction of flavonoid than the shake flask method.

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Section: Introductionmentioning

confidence: 99%

Simultaneous Extraction Optimization and Analysis of Flavonoids from the Flowers of Tabernaemontana heyneana by High Performance Liquid Chromatography Coupled to Diode Array Detector and Electron Spray Ionization/Mass Spectrometry

Sathishkumar¹,

Baskar²,

Aravind³

et al. 2013

ISRN Biotechnology

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“…Although we found geniposide in the extract of B5, it could not be proven that geniposide indeed existed in the extract just based on molecular mass. Other methods must be used to obtain further conWrmation, for geniposide has anti-inXammatory activity, and it was found to be the main hypotensive compound in Eucommia ulmoides Oliver [24]. Therefore, it is highly likely that the strain B5 could produce the bioactive component, chlorogenic acid, just like its host, Eucommia ulmoides Oliver, does.…”

Section: Discussionmentioning

confidence: 96%

“…The principal components are geniposidic acid and chlorogenic acid [9,10,14,18]. Nowadays, chlorogenic acid is widely used for its antimicrobial, anti-inXammatory, antioxidant, anticancer, and anti-hepatitis B virus activities [3,13,20,23,24]. Chlorogenic acid has now been oYcially recorded in the National Pharmacopoeia of China [2].…”

Section: Introductionmentioning

confidence: 99%

Studies on a chlorogenic acid-producing endophytic fungi isolated from Eucommia ulmoides Oliver

Chen

Sang

et al. 2010

J Ind Microbiol Biotechnol

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Eucommia ulmoides Oliver is a traditional medicinal plant of China, and it is one of the main sources of chlorogenic acid. Chlorogenic acid is an ester of caffeic acid, quinic acid, and a phenolic compound that has antibacterial, antifungal, antioxidant, and antitumor activities. The purpose of this study was to determine whether endophytic fungi isolated from Eucommia ulmoides Oliver had the same ability to produce chlorogenic acid. Primary screening was done by antibacterial and antifungal reactions, and the strain reselection was done with high-performance liquid chromatography (HPLC) to identify the fermentation products of the selected strains. Extracts of the leaf and cortex of Eucommia ulmoides Oliver were also deteted by HPLC, then positive results of HPLC were analyzed by GC-MS and LC-MS. In this study, 29 strains were isolated from Eucommia ulmoides Oliver. Most of them had antibacterial activity, and a few of them had antifungal activity. One ingredient of the B5 extract had a retention time identical to that of authentic chlorogenic acid. With GC-MS, other ingredients, isocoumarin and p-chlorocinnamide, were found. With LC-MS, chlorogenic acid and geniposide related to Eucommia ulmoides Oliver were found. The strain B5 was identified as Sordariomycete sp. Thus, endophytic fungi may produce the bioactive compound chlorogenic acid, as their host plant does, and could be used for the production of chlorogenic acid by fermentation in the future.

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“…indicated that the principal components of E. ulmoides Oliver. were geniposidic acid and chlorogenic acid, moreover, chlorogenic acid was widely used for its antimicrobial, anti-inxammatory, antioxidant, anticancer, and anti-hepatitis B virus activities (Riitta et al, 2005;Wu et al, 2007). Therefore, the next goal is to isolate and purify the acetyl acetate extract in order to confirm whether chlorogenic acid exists in the acetyl acetate extract or obtain other compounds with antifungal activities.…”

Section: Discussionmentioning

confidence: 99%

Studies on some active components and antimicrobial activities of the fermentation broth of endophytic fungi DZY16 Isolated from Eucommia ulmoides Oliv.

Ding¹,

Sun²,

Xia³

et al. 2013

Afr. J. Biotechnol.

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Research into plant-derived endophytic fungi has grown in recent decades. Endophytic fungi still have enormous potential to inspire and influence modern agriculture. In this study, the endophytic fungi DZY16 isolated from Eucommia ulmoides Oliv. was tested for its bioactive components and antimicrobial activities using phenol-sulfuric acid method, high performance liquid chromatography method and growth inhibition measurements. The results show that variation trend of extracellular polysaccharide content at different growth stages of the strain DZY16 and the maximum content of extracellular polysaccharide was 2.02 g/L at the sixth day. Moreover, the fermentation broth of the DZY16 contained guanosine, uridine and adenosine; the contents were 1.54 mg/g, 1.07 mg/g and 1.36 mg/g respectively. On the other hand, the strongest antimicrobial activity was exhibited by the acetylacetate extract of strain DZY16 against Rhizoctonia solani and Gibberella zeae, showing 59.84 and 70.86% respectively. The strain DZY16 was identified by internal transcribed spacer (ITS) sequence as belonging to Nigrospora. The results indicate that the endophytic fungi DZY16 of the plant E. ulmoides Oliv. is a promising source of novel bioactive compounds.

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Comparison of techniques for the extraction of the hypotensive drugs geniposidic acid and geniposide from Eucommia ulmoides

Cited by 14 publications

References 25 publications

Simultaneous Extraction Optimization and Analysis of Flavonoids from the Flowers of Tabernaemontana heyneana by High Performance Liquid Chromatography Coupled to Diode Array Detector and Electron Spray Ionization/Mass Spectrometry

Simultaneous Extraction Optimization and Analysis of Flavonoids from the Flowers of Tabernaemontana heyneana by High Performance Liquid Chromatography Coupled to Diode Array Detector and Electron Spray Ionization/Mass Spectrometry

Studies on a chlorogenic acid-producing endophytic fungi isolated from Eucommia ulmoides Oliver

Studies on some active components and antimicrobial activities of the fermentation broth of endophytic fungi DZY16 Isolated from Eucommia ulmoides Oliv.

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