Research into plant-derived endophytic fungi has grown in recent decades. Endophytic fungi still have enormous potential to inspire and influence modern agriculture. In this study, the endophytic fungi DZY16 isolated from Eucommia ulmoides Oliv. was tested for its bioactive components and antimicrobial activities using phenol-sulfuric acid method, high performance liquid chromatography method and growth inhibition measurements. The results show that variation trend of extracellular polysaccharide content at different growth stages of the strain DZY16 and the maximum content of extracellular polysaccharide was 2.02 g/L at the sixth day. Moreover, the fermentation broth of the DZY16 contained guanosine, uridine and adenosine; the contents were 1.54 mg/g, 1.07 mg/g and 1.36 mg/g respectively. On the other hand, the strongest antimicrobial activity was exhibited by the acetylacetate extract of strain DZY16 against Rhizoctonia solani and Gibberella zeae, showing 59.84 and 70.86% respectively. The strain DZY16 was identified by internal transcribed spacer (ITS) sequence as belonging to Nigrospora. The results indicate that the endophytic fungi DZY16 of the plant E. ulmoides Oliv. is a promising source of novel bioactive compounds.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.