Comparison of serological tests for Trypanosoma evansi natural infections in water buffaloes from north Vietnam

Verloo, Didier; Holland, Wicher; My, Ln; Thanh, Ng; Tam, PT; Goddeeris, Bruno; Vercruysse, Jozef; Büscher, P.

doi:10.1016/s0304-4017(00)00284-3

Cited by 84 publications

(55 citation statements)

References 15 publications

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“…The optimum test serum dilution found in the present study was 1:10. However, little higher or lower dilutions reported by previous workers as (1:50) by Jithendran et al (1997), (1:100) by Desquesnes et al (2009), (1:200) by Shahardar et al (2008) and (1:400) by Verloo et al (2000). The optimum dilution of 1: 6,000 rabbit anti-ovine Ig G-HRPO conjugate used in the study is in agreement with Jithendran et al (1997).…”

Section: Resultssupporting

confidence: 81%

“…The optimum concentration of WCL Ag of T. evansi for coating the wells of ELISA plate determined in the study as 5 lg/ml is in agreement with findings of Desquesnes et al (2009) (5 lg/ml) and Shahardar et al (2008) (5 lg/ml). However, Jithendran et al (1997) used 10 lg/ml and Verloo et al (2000) used 2 lg/ml for coating of micro plate wells. The optimum test serum dilution found in the present study was 1:10.…”

Section: Resultsmentioning

confidence: 99%

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Diagnosis of Trypanosoma evansi in bovines by indirect ELISA

et al. 2014

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The diagnosis of Trypanosoma evansi in animals with low parasitaemia is hampered by low diagnostic sensitivity of traditional detection methods. The present study was undertaken with an objective to improve the diagnostic tools for detection of antibodies against T. evansi infection using indirect enzyme-linked immunosorbent assay (ELISA) in bovines. The optimum concentration of antigen, test sera and conjugate were determined as 5 lg per well, 1:10 and 1: 6,000 dilutions, respectively. Among 320 cattle and 382 buffaloes examined in different parts of Rayalaseema region of Andhra Pradesh for T. evansi infection, 36.12 and 31.87 percent were found positive by indirect ELISA, respectively.

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Section: Resultssupporting

confidence: 81%

Section: Resultsmentioning

confidence: 99%

Diagnosis of Trypanosoma evansi in bovines by indirect ELISA

et al. 2014

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“…The lowest detection limit of Woo's test was estimated at 30 trypanosomes per milliliter of blood (16). Verloo et al (6) reported that water buffaloes positive for T. evansi by the mouse inoculation test were negative by Woo's test.…”

Section: Discussionmentioning

confidence: 99%

Field investigation of Trypanosoma evansi and comparative analysisof diagnostic tests in horses from Bahawalpur, Pakistan

Tehseen¹,

Jahan²,

Desquesnes³

et al. 2017

Turk J Vet Anim Sci

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In order to assess the prevalence of Trypanosoma evansi, a parasitological, molecular, and serological-based investigation was carried out in randomly sampled horses (n = 375) belonging to different age groups, sexes, and localities from the district of Bahawalpur, Pakistan. The diagnostic performance of applied tests was also compared. The prevalence was recorded as 0.5% with Woo's test, 1.3% with both ITS CF/BR PCR and RoTat 1.2 PCR, and 14.4% with CATT/T.evansi. Based on CATT/T.evansi, significant differences (P ≤ 0.05) were observed for prevalence estimates according to different localities, sexes, body condition scores, and origins. A nonsignificant difference (P ≥ 0.05) was observed among different age groups and variable parity numbers. Our study declares district Bahawalpur to be a high risk area for surra and proposes the potential use of CATT/T.evansi as a field test of choice for surveys in horses. However, the status of seropositive animals should be confirmed using a more sensitive molecular tool such as the satellite DNA target. A widespread status of anti-Trypanosoma antibodies calls for control measures and further investigation of various species (camels, cattle, sheep, goats, buffaloes) inhabiting the same area to identify reservoir status.

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“…For the development of simple and easy-to-use agglutination assays, latex bead technology has been used successfully (24,36,59,64,65). Similarly, recombinant or synthetic peptides derived from species-specific M. bovis proteins containing epitopes recognized by antibodies from infected animals can be used singly or multiplexed on a lysine polymer backbone using currently available technology to detect antibodies specific for M. bovis (22).…”

Section: Discussionmentioning

confidence: 99%

Use of rMPB70 Protein and ESAT-6 Peptide as Antigens for Comparison of the Enzyme-Linked Immunosorbent, Immunochromatographic, and Latex Bead Agglutination Assays for Serodiagnosis of Bovine Tuberculosis

et al. 2005

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Current assays used to detect Mycobacterium bovis infection lack accuracy, especially for recently infected animals, or are impractical for rapid field diagnostic applications. To overcome these limitations with serological assays, a synthetic peptide derived from early secretory antigenic target 6 (ESAT6-p) and a recombinant major secreted immunogenic protein (rMPB70) of M. bovis were used in an enzyme-linked immunosorbent assay (EIA), an immunochromatographic assay (ICGA), and a latex bead agglutination assay (LBAA). Sera from noninfected, M. bovis-infected, or M. avium subsp. Bovine tuberculosis (bTB) is caused by Mycobacterium bovis, a member of the Mycobacterium tuberculosis complex. bTB is one of the important bovine diseases causing great economic loss worldwide (21, 30). These are also diseases of concern for other ruminant species and humans (8, 21). The presence of M. bovis in wild-animal reservoirs (21,23,32,38,49) has also made control of the diseases more difficult.At present, the standard diagnostic assay for bTB is the single intradermal (SID) test (skin test) with purified protein derivative (PPD). There are many limitations to the SID test and other cell-mediated immunity (CMI)-based tests (e.g., gamma interferon [IFN-␥] and lymphocyte proliferation assays) for the diagnosis of bTB. The first limitation is that the delayed-type hypersensitivity response to the antigen has low sensitivity and specificity. It is not possible to determine if the response to PPD is attributable to exposure to M. bovis, M. avium subsp. paratuberculosis, or environmental mycobacteria, e.g., Mycobacterium avium subsp. avium. Second, there is a predominant CMI response during the early phases of infection with low bacillary loads which may be reduced or absent in animals at the advanced stages of disease with high bacterial loads. The animals become anergic to CMI-based tests (18,35,42,46). Third, animals need to be handled twice over a 72-h period to obtain results of the test. Fourth, other CMI-based tests are expensive and require trained staff to perform the tests and interpret the results. Fifth, false positives may result from exposure to environmental mycobacteria and/or vaccination with BCG. Therefore, there is a need to develop sensitive and easy-to-use M. bovis-specific serological tests that can be used to distinguish between animals exposed to M. bovis, M. avium subsp. paratuberculosis, and environmental mycobacteria.

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Comparison of serological tests for Trypanosoma evansi natural infections in water buffaloes from north Vietnam

Cited by 84 publications

References 15 publications

Diagnosis of Trypanosoma evansi in bovines by indirect ELISA

Diagnosis of Trypanosoma evansi in bovines by indirect ELISA

Field investigation of Trypanosoma evansi and comparative analysisof diagnostic tests in horses from Bahawalpur, Pakistan

Use of rMPB70 Protein and ESAT-6 Peptide as Antigens for Comparison of the Enzyme-Linked Immunosorbent, Immunochromatographic, and Latex Bead Agglutination Assays for Serodiagnosis of Bovine Tuberculosis

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