Comparison of Rumen Microbiota and Serum Biochemical Indices in White Cashmere Goats Fed Ensiled or Sun-Dried Mulberry Leaves

Abstract

<jats:p>Mulberry leaves, which have high nutritional value, have not been fully utilized. Few research systems have indicated whether mulberry leaves can replace traditional feed ingredients in goats. In this study, we investigated the effects of feeding white cashmere goats ensiled (Group E) or sun-dried mulberry leaves (Group S) on changes in ruminal microbial communities, rumen fermentation parameters and serum biochemical indices. The control group (Group C) received a typical total mixed ration (TMR). 16S rRNA gene sequencing revealed 209 genera belonging to 19 bacterial phyla dominated by Firmicutes and Bacteroidetes. Only the relative abundances of Erysipelotrichaceae_UCG-009 were significantly different among the three groups (p &lt; 0.05). Physiological and biochemical findings revealed that only the serum leptin concentrations were significantly decreased when mulberry leaves were added to the diets (p &lt; 0.05). Correlation analysis revealed that Ruminococcus_2 were significantly positively correlated with the butyrate concentration. These findings suggested that supplementation with mulberry leaves only induced minor changes in the abovementioned indicators, implying that the rumen fermentation status was still stable after adding mulberry leaves to the diets.</jats:p>

Effects of Phragmites australis Shoot Remainder Silage on Growth Performance, Blood Biochemical Parameters, and Rumen Microbiota of Beef Cattle

“…In the current study, the concentration of GLU in the mixed group was significantly lower than in the PSR silage and corn silage treatments, while the higher ADG was observed in the mixed group. These results were inconsistent with previous studies, and this might be related to species and feed composition ( 11 – 15 ). The concentration of BUN is perceived as an effective indicator to measure the metabolism of protein and amino acid, low BUN level indicates high nitrogen metabolism capacity ( 21 ).…”

“…More than 70% of the energy was provided by rumen bacteria fermentation to ensure the host growth and reproduction performance ( 23 ), and the compositions of rumen microorganism are influenced by diets ( 24 ). In the present study, the core microbiome accounts for more than 78% of total OTUs among the three groups, and this result was similar with other researches ( 11 – 13 ). The core microbiome plays crucial roles in maintaining the “functional redundancy” of rumen ecosystem, and this redundancy further guarantees its major functional properties ( 25 ).…”

“…After standing for 2-3 h, blood samples were centrifuged by the model TG16-WS H1650 centrifuges (Hunan Xiangyi Laboratory Instrument Development Co. Ltd.) at 3,000 r/min for 10 min, and then the supernatant was separated and stored at −20 • C for further analysis. A TBA-120FR automatic biochemistry analyzer (Toshiba Corporation) was used to measure the concentrations of serum biochemical parameters (11)(12)(13).…”

Characterization of the Bacterial Community of Rumen in Dairy Cows with Laminitis

“…Saccharofermentans belongs to the Bacteroidetes phylum and participates in hemicellulose, pectin, arabinogalactan, starch, fructan, and chitin degradation [ 31 ]. In addition, this study showed that Erysipelotrichaceae UCG-009 was positively associated with the production of butyrate [ 32 ]. At the species level, Acetobacter pasteurianus , Clostridium papyrosolvens , Ruminococcaceae bacterium AE2021 , Porphyromonas crevioricanis , Pseudomonas boreopolis , Pseudomonas psychrotolerans , Rothia nasimurium , and Rothia pickettii increased, while the relative abundance Alysiella_crassa was reduced.…”

Lactiplantibacillus plantarumCCFM1019 attenuate polycystic ovary syndrome through butyrate dependent gut–brain mechanism

“…8,21,30 Both Lachnospira and Ruminococcus_2 are considered to be SCFA producers. 34,35 A positive correlation has also been identified between these taxa and butyrate levels in both animal models 36 and human trials. 37 Moreover, the relative abundance of Lachnospira has been found to be decreased in PCOS patients, 12 but this was not observed in our model.…”