Characterization of the Bacterial Community of Rumen in Dairy Cows with Laminitis

Abstract

Laminitis is the inflammation of the lamella, and it has caused great economic loss to the dairy industry and attracted wide attention around the world. In recent years, microbiota are considered to play a significant role in various diseases processes. Therefore, our study aimed to explore the characteristics of ruminal microbiota in laminitis cows. The serum of bovines with or without laminitis was collected to detect concentrations of lipopolysaccharide (LPS), lactic acid, and histamine, and ruminal fluid was collected for 16S rDNA sequence analysis. The results showed a significant increase in LPS and lactic acid levels in the laminitis group compared to the control group cows. In addition, a higher abundance of Candidatus Saccharimonas, Saccharofermentans, Erysipelotrichaceae UCG-009 genus, Acetobacter pasteurianus, Clostridium papyrosolvens, Ruminococcaceae bacterium AE2021, Porphyromonas crevioricanis, Pseudomonas boreopolis, Pseudomonas psychrotolerans, Rothia nasimurium, and Rothia pickettii was detected in the rumen fluid of laminitis bovines. In conclusion, this article confirms that there are differences in rumen microbiota between healthy and laminitis bovines. The elevated abundance of bacteria that enrich acid-enhancing metabolites, as well as increase the concentration of lactic acid and LPS, could be harmful factors to bovines and increase the risk of laminitis.

Differential Responses of Digesta- and Mucosa-Associated Jejunal Microbiota of Hu Sheep to Pelleted and Non-Pelleted High-Grain Diets

“…was confirmed in a previous study, and always isolated from some human clinical subjects [60]. In addition, Candidatus Saccharimonas were found to proliferate in the intestinal lumen of parasite-infected sheep [61] and rumen of dairy cows suffering from laminitis [39]. Therefore, consistent with the changes observed in the digesta-associated microbiota, these results showed that an HP diet seemed to be able to partially alleviate the adverse effects of HG diets.…”

“…Strains of Acetitomaculum were obligate anaerobe and fermented various substrates (such as glucose, fructose, and cellobiose) to acetate in the rumen of ruminants [36,37]. The evidence indicated that the ruminal Candidatus Saccharimonas was significantly and positively correlated with proportion production [38], and a significant increase in these microorganisms in the rumen of dairy cows suffering from laminitis has been reported [39]. Meanwhile, we also found a significant decrease in some beneficial microorganisms, such as Christensenellaceae R-7 group and Blautia, in the animals in the HG diets group compared to those in the CON group.…”

Gut microbiota plasticity in insular lizards under reversed island syndrome

“…The former characterises the microbial community of vertebrates (i.e. 60 ), and therefore we excluded that its presence in gut microbiota of lizards was due to a conditioning by the microbiota of the moth.…”

The Role of Rumen Microbiota and Its Metabolites in Subacute Ruminal Acidosis (SARA)-Induced Inflammatory Diseases of Ruminants

“…SARA causes the pH of the system to decrease and subsequently activates vascular activity, increasing blood pressure in the vascular system of the hoof. Meanwhile, rumen endotoxins and histamine are released into the bloodstream, increasing vasoconstriction and dilation [ 39 ]. Subsequent vascular damage with oedema, dermal congestion and even thrombus formation eventually leads to dilation of the dermis and increasing severity of laminitis [ 40 ].…”

“…The critical link between lactic acidosis and laminitis appears to be associated with persistent hypoperfusion, which results in ischemia in the digit [ 42 ]. In addition, our recent study found that laminitis in dairy cows was not only associated with elevated levels of lactate and LPS but also closely related to the bacterial community of the rumen, characterized by elevated abundance of bacteria that enrich acid-enhancing metabolites [ 39 ].…”