Differential Responses of Digesta- and Mucosa-Associated Jejunal Microbiota of Hu Sheep to Pelleted and Non-Pelleted High-Grain Diets

Abstract

In the present study, we utilized 16S rRNA sequencing to uncover the impacts of non-pelleted (HG) or high-grain pelleted (HP) diets on the microbial structure and potential functions of digesta- and mucosa-associated microbiota in the jejunum of Hu sheep. Here, we randomly assigned 15 healthy male Hu sheep into three groups and fed the control diets (CON), HG, and HP diets, respectively. The experiment period was 60 days. The HP diets had the same nutritional ingredients as the HG diets but in pelleted form. At the finish of the experiment, the jejunal digesta and mucosa were gathered for microbial sequencing. The results of PCoA and PERMANOVA showed that different dietary treatments had significant impact (p < 0.05) on digesta- and mucosa-associated microbiota in the jejunum of Hu sheep. For specific differences, HG diets significantly increased (p < 0.05) the abundance of some acid-producing bacteria in both jejunal digesta (Bifidobacterium, OTU151, and OTU16) and mucosa (Rikenellaceae RC9 gut group, and Bifidobacterium) of Hu sheep compared with the CON diets. Besides the similar effects of the HG diets (increased the acid-producing bacteria such as Olsenella, Pseudoramibacter, and Shuttleworthia), our results also showed that the HP diets significantly decreased (p < 0.05) the abundance of some pro-inflammatory bacteria in the jejunal digesta (Mogibacterium, and Marvinbryantia) and mucosa (Chitinophaga, and Candidatus Saccharimonas) of Hu sheep compared with the HG diets. Collectively, these findings contributed to enriching the knowledge about the effects of HG diets on the structure and function of intestinal microbiota in ruminants.

Oregano Essential Oil as a Natural Plant Additive Affects Growth Performance and Serum Antibody Levels by Regulating the Rumen Microbiota of Calves

“…Through the screening of the correlation network diagram between species, the core species with the highest number of inter-species associations in CON was g_Mogibacterium , a pro-inflammatory bacterium present in the digestive tract of ruminants [ 73 ], and in the group supplemented with oregano essential oil, the core species was g_Ruminococcus , which plays a significant role in the rumen of ruminants in the production of short-chain fatty acids [ 74 ] and the degradation of cellulose [ 75 ]. This suggests that oregano essential oil modulates the structural function of rumen microbes in calves and promotes gastrointestinal digestion.…”

Impact of 5–20% Hydroponic Wheat Sprouts Inclusion on Growth and Metabolic Parameters of Growing Ewes

“…It was found that an increase in the abundance of Olsenella was considered to improve immunity in calves [ 21 ]. Moreover, Olsenella was also considered to strengthen the immunoregulation capacity of a goat model [ 22 ] and mouse model [ 23 ] host. Similarly, Limosilactobacillus has been proved to have antibacterial and intestinal immunity-enhancing effects [ 24 ].…”

Successional action of Bacteroidota and Firmicutes in decomposing straw polymers in a paddy soil

“…5 A). Chitinophagales are rarely detected in gut [ 56 , 57 ], and to our knowledge, have not been documented in plant polymer decomposition in paddy soils. Currently, only a small number of Chitinophaga species have been isolated and characterized from soil environments [ 58 – 60 ] and most of these cultured species are aerobes that respire plant sugar polymers with oxygen [ 61 – 63 ].…”