Comparison of ractopamine residue depletion from internal tissues

Pleadin, Jelka; Vulić, Ana; Perši, Nina; Terzić, Svjetlana; Andrišić, Miroslav; Žarković, Irena; Šandor, Ksenija; Perak, Eleonora

doi:10.3109/08923973.2012.702115

Cited by 7 publications

(5 citation statements)

References 23 publications

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“…Overall. It is clear that the risk of exposure to ractopamine residues is greater in goat tissues than in sheep tissues (4,25). The residues in liver and became undetectable after 95 h in goat and 82 h in the sheep, similar to delays reported for clenbuterol, a β 2 -agonist, in goat tissues (8,26).…”

Section: Discussionsupporting

confidence: 64%

Assessment of the withdrawal period for ractopamine hydrochloride in the goat and sheep

Lazuardi¹,

Hermanto²,

Restiadi³

2020

IJVS

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Ractopamine hydrochloride often used as a bronchodilator, but its β-adrenergic agonist effects on un-striated muscle and its withdrawal time have not been assessed for Etawah goats and sheep. The aim of this study was to determine the safe time to slaughter goats and sheep post-treatment with ractopamine. Five clinically healthy adult goats and sheep (20 kg body weight) were treated with a single dose of ractopamine (1 mg, intravenously). Whole blood was sampled from the jugular vein at 120 min, 180 min and 300 min post-treatment. Ractopamine as a veterinary drug was analysed using HPLC at wavelength 225 nm.

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Section: Discussionsupporting

confidence: 64%

Assessment of the withdrawal period for ractopamine hydrochloride in the goat and sheep

Lazuardi¹,

Hermanto²,

Restiadi³

2020

IJVS

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“…The absorption and metabolism of different β-receptor agonists in rats are quite different, and the absorption and metabolism residues of the same β-receptor agonist in different tissues are also different [ 43 , 48 , 49 ]. The residues of SAL in broilers are liver > kidney > muscle [ 37 ]; the concentrations of SAL in pig liver and kidney 6 h after enzymolysis treatment are: 37.49 ± 4.61 μg/kg and 37.98 ± 4.61 μg/kg, respectively; the concentrations in the liver and kidney of sheep 6 h after enzymolysis treatment were 55.08 ± 14.8 μg/mL and 65.49 ± 6.75 μg/mL, respectively.…”

Section: Discussionmentioning

confidence: 99%

Determination of the Metabolites and Metabolic Pathways for Three β-Receptor Agonists in Rats Based on LC-MS/MS

Liang

Wang

Zhang

et al. 2022

Animals

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This paper developed a universal detection method by high-performance liquid chromatography-tandem mass spectrometry to detect three typical clenbuterols, CLB, SAL, and RAC, and to investigate the metabolism of β-agonists in vivo. The parent ions and daughter ions of the three β-receptor agonist standards and the residues in the muscle, liver, and blood samples of rats were obtained by Total Ions Scan mode. The metabolites produced in different tissues at a specific time were qualitatively and quantitatively analyzed, and the corresponding metabolic pathways were inferred. The results showed that the three β-receptor agonists mainly existed in the form of prototype drugs in rats, with a small amount of clenbuterol methyl compound and albuterol methyl compound. There were significant differences in residual metabolism between different tissues of the same species. In addition, different β-receptor agonists have different absorption and utilization rates in rats.

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“…Various methods for detecting residue of β2agonists have developed, namely immunoassay (Pleadin et al, 2013), chemiluminescent (Gao et al, 2014), molecularly imprinted polymer (Tang et al, 2018) and high-performance liquid chromatography (HPLC) with ultraviolet detectors (Du et al, 2013;Yan et al, 2016), or fluorescence detectors (FLD) (Hu et al, 2010). Among these methods, HPLC is one of the most widely used methods.…”

Section: Introductionmentioning

confidence: 99%

Salbutamol Residue in Plasma and Urine of Balinese Calves after Single-Dose Administration

Anastasia

Latif²,

Sutardi³

et al. 2021

Acta Vet Indones

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Salbutamol, a short-acting β2-adrenergic receptor agonist, due to illegal use in animal feed, is very dangerous for the safety of animal origin products. The purpose of this study is to determine the residue of salbutamol in plasma and urine of balinese calf after a single-dose administration. Three calves were given of feed fortification of salbutamol (10 mg/kg body weight). The salbutamol concentrations were measured in the plasma and urine. Samples were extracted with perchloric acid and purified by cation exchange solid phase extraction (SPE), then analyzed by High-Performance Liquid Chromatography (HPLC) with a photodiode array detector using an RP C18 column with a mixed mobile phase of purified water pH 3.0 (adjusted with phosphoric acid) and acetonitrile (90:10, v/v). The highest salbutamol concentration in the plasma sample was 0.28±0.15 μg/mL at 12 hours and decreased after 24 hours after feeding. The highest concentration of salbutamol in urine was 15.85±4.42 μg/mL at 18 hours and decreased gradually starting at 24 hours. This result concluded that salbutamol residues mostly excreted in the urine, and the highest salbutamol residues in plasma samples formed faster but also decreased more rapidly than residues formed in urine. The residue formed in plasma is lower than that in the urine.

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Comparison of ractopamine residue depletion from internal tissues

Cited by 7 publications

References 23 publications

Assessment of the withdrawal period for ractopamine hydrochloride in the goat and sheep

Assessment of the withdrawal period for ractopamine hydrochloride in the goat and sheep

Determination of the Metabolites and Metabolic Pathways for Three β-Receptor Agonists in Rats Based on LC-MS/MS

Salbutamol Residue in Plasma and Urine of Balinese Calves after Single-Dose Administration

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