“…Utilizing high-performance thin layer chromatography and various mobile phase solvents, such as 1-butanol-chloroform-acetic acid-ammonia-water, 7 : 4 : 5 : 1 : 1, or benzene-methanol-acetone-acetic acid, 70 : 20 : 5 : 5, or chloroform-ethanol-acetone-ammonia, 2 : 2 : 2 : 1, achieved some useful level of separation for riboflavin identification [ 10 ]. Other various liquid chromatography approaches were able to extract vitamins B-1 and B-2 from foods for determination [ 11 , 12 ] and use reverse-phase chromatography with 254 nm detection [ 13 ], biosensor technology [ 14 ], ion-pair chromatography [ 15 , 16 ], determination in dairy products [ 17 ], foodstuffs [ 18 ], detection of casein [ 19 ], total riboflavin in foods [ 20 ], riboflavin in infant formula [ 21 , 22 ], and simultaneous thiamine, riboflavin, and niacin in food [ 23 ]. Methods of a riboflavin assay that utilize UV/visible spectrometry with solvation into aqueous media for speed, ease of application, low cost, high sensitivity, accuracy, and extensive application are very few.…”