“…The preparation of the sample is the critical step from a practical point of view, involving a time-consuming and manual procedure. The determination of RF and its metabolites in complex food matrices is normally carried out by a previous extensive sample extraction, as a clean-up procedure, to remove the major matrix interferences without producing the hydrolysis of flavin compounds [24,27,29,30,33]. All these methods are labor-intensive, as they are implemented in an off-line mode (commonly involving high human manipulation), and they are characterized to be timeconsuming.…”