Detection and Assay of Vitamin B-2 (Riboflavin) in Alkaline Borate Buffer with UV/Visible Spectrophotometry

Abstract

The detection and assay of vitamin B-2 (riboflavin) was accomplished under aqueous conditions using sodium borate buffering at pH 7.52 conditions. The absorbance spectrum of riboflavin was determined at different pH values utilizing several buffers. The buffer at pH at 7.52 is followed by accurate and sensitive assay of riboflavin by spectrophotometer at 440 nm wavelength. Where indicated an origin solution (stock) was employed by dissolving sufficient vitamin to make a stock solution of 1.403 × 10−4 molar concentrations. Measurements of various aqueous solutions containing riboflavin were accomplished that included aqueous test samples, vitamin capsules/tablets, and water vitamin mixtures. A standard curve extended from 7.97 × 10−7 molar to 1.23 × 10−4 molar (a 154x folds spread in concentration). The equation of the line was y = 12545x (intercept at origin) with Pearson r correlation of 1.000 (R 2 = 1.000). Concentration of riboflavin assayed ranged from 3.00 × 10−4 gram per liter (0.30 ppm) to 0.0463 gram per liter (46.35 ppm). The B vitamin riboflavin can be assayed by UV/VIS spectrophotometer at 440 nm in aqueous media and using sodium borate buffer at pH 7.52. The assay can reach as low as 0.30 parts per million with high levels of accuracy and sensitivity.

Evaluation of cell wall-associated direct extracellular electron transfer in thermophilic Geobacillus sp.

“…Furthermore, ribo avin also yields three absorption peaks between 250 to 500 nm at 270, 350, and 446 nm. Similar absorption peaks were also observed in ribo avin using sodium borate buffer at pH 7.52 (Bartzatt and Wol 2014). However, none of the other spectral peaks was found.…”

Optimizing the Efficiency of a Cytocompatible Carbon-Dots-Based FRET Platform and Its Application as a Riboflavin Sensor in Beverages

“…The nanoprobe provides a simple approach to creating a ratiometric fluorescent sensor based on the FRET process for the determination of RF in aqueous media. Capillary Electrophoresis/Laser-Induced Fluorescence 0.001-1.33 0.008 [15] HPLC/tandem mass spectrometry 0.011-53.1 0.029 [12] Ag nanoparticles modified with β-CD/Colorimetry 0.16-4.66 0.167 [36] Spectrophotometry/Absorbance at 440 nm 0.797-123 0.79 [11] Graphitic carbon nitride/Fluorimetry (FRET) 0.4-10 0.170 [16] Carbon-dot-codoped N, P/Fluorimetry (FRET) 0.5-50 0.170 [17] g-CNQDs@Zn-MOF/Fluorimetry (FRET) 0-1 0.015 [18] Sulfonated graphene/Fluorimetry (FRET) 0.4-10 1.6 [37] N,S-doped carbon dots/Fluorimetry (FRET) 0.56-7.4 1.9 [19] Graphene-PEG/Fluorescence Quenching 0-6.3 60 [38] Molecularly Imprinted Polymer (PIM)/Fluorimetry Not reported 79.7 [39] Carbon dots (N, COO − )/Fluorimetry (FRET) 0-11 0.025 This work…”

“…It has been reported that RF can also prevent different types of cancers [10]. Accordingly, the determination of RF has become very important and, therefore, different methods have been developed for the determination of RF, including spectrophotometric methods [11], high-performance liquid chromatography [12], electrochemical methods [13,14], and capillary electrophoresis [15]. However, these methods have multiple shortcomings, including a slow response, low selectivity, the use of a large amount of solvent, high-cost equipment, and laborious procedures.…”

Addition of Riboflavin-Coupled Magnetic Beads Increases Current Production in Bioelectrochemical Systems via the Increased Formation of Anode-Biofilms

“…The beads were then separated from the riboflavin solution using a magnetic separator. Riboflavin was quantified using a plate reader (Infinite Pro M200 Tecan, Switzerland) at 440 nm (Bartzatt and Wol, 2014). The riboflavin concentration was determined based on a calibration curve.…”