Comparison of MAST With Radioallergosorbent and Skin Tests for Diagnosis of Allergy in Children

Ownby, Dennis R.

doi:10.1001/archpedi.1986.02140150047031

Cited by 16 publications

(7 citation statements)

References 9 publications

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“…In their study of 20 atopic children. Ownby et al found agreement between MAST and RAST for five common allergens to be 90"/(, [15]. Brostoff et al using sera from 75 atopic subjects showed MAST and RAST results to give either identical class results, or results differing by only one class in 80% of cases on testing for eight allergens [16], Moreover, both tests have a similar agreement with clinical status.…”

Section: Discussionmentioning

confidence: 98%

Relationship between skin‐prick tests, the multiple allergosorbent test and symptoms of allergic disease

Finnerty

Summerell

Holgate

1989

Clin Experimental Allergy

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In 47 atopic subjects, skin-prick testing to 10 common allergens was performed, and specific IgE to the same allergens was assessed by the multi-allergosorbent chemiluminescent assay (MAST-CLA). Overall agreement between the tests was 66.4% for conventionally positive skin tests (weal diameter greater than or equal to 3 mm), rising to 78.5% when a positive skin test was defined as having a weal diameter greater than or equal to 5 mm. Agreement between the tests was statistically significant for all allergens except Alternaria. A history was obtained from each subject of the presence or absence of allergic symptoms on exposure to cats, and whether there was a history of grass pollen allergy. MAST-CLA testing for specific IgE to cat dander predicted a history of cat allergy with an efficiency of 74.5%, while a positive MAST-CLA test for Cocksfoot grass predicted a history of grass pollen allergy with an efficiency of 85.1%. Similar results were obtained on skin testing for these allergens. We conclude that MAST-CLA gave results comparable to those obtained by skin-prick testing, and correlated equally well with the history of allergic symptoms.

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Section: Discussionmentioning

confidence: 98%

Relationship between skin‐prick tests, the multiple allergosorbent test and symptoms of allergic disease

Finnerty

Summerell

Holgate

1989

Clin Experimental Allergy

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“…In vitro methods measure circulating IgE in serum; however, in practice, most IgE is attached to the mast cells of the tissue; therefore, the IgE level can be underestimated by in vitro methods (lower sensitivity, false negativity) [5,33]. Conversely, in in vivo methods, the positive detection rate increases due to non-IgE-mediated triggers or alternative mechanisms (lower specificity, false positivity) [5,34]. There were 8.3% (n = 10) of patients with increased TIgE levels, although no SIgE was detected.…”

Section: Discussionmentioning

confidence: 99%

Multiple Allergen Simultaneous Test-Immunoblot Assay for Immunoglobulin E Detection in Patients with Isolated Allergic Conjunctivitis

Han

Lee

Chung³

et al. 2021

JCM

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We aimed to investigate the immunoglobulin E (IgE) detection rate and allergen patterns in patients with isolated allergic conjunctivitis using the multiple allergen simultaneous test (MAST)-immunoblot assay. A total of 120 patients with allergic conjunctivitis and no associated rhinitis, asthma, or dermatitis underwent the MAST-immunoblot assay to measure serum total IgE (TIgE) and serum specific IgE (SIgE) against 57 allergens. Patients were classified into subgroups based on the season when the eye symptoms were exacerbated, and TIgE and SIgE positivity. Differences between sex and age groups were also analyzed. Of the 120 patients, 57.5% (69 patients) and 69.2% (83 patients) were positive for TIgE (≥100 IU/mL) and SIgE (≥0.7 IU/mL), respectively. The allergens that most frequently triggered sensitization in the study population were Dermatophagoides farinae, Dermatophagoides pteronyssinus, Tyrophagus putrescentiae, Alternaria, and house dust. House dust mites, such as D. farinae and D. pteronyssinus, showed the highest detection rates regardless of the season. Men had a higher positive rate for TIgE than women, whereas a higher rate of sensitization, detected as SIgE positivity, was seen in younger patients. In conclusion, MAST-immunoblot assay can detect sensitizing allergens in patients with isolated allergic conjunctivitis.

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“…Strong correlation between positive MAST and positive RAST has been reported with agreement of 80% to 90% for common allergens [9,[15][16][17], even if a correct comparison should require the use of reagents with the same allergens. Also in this study that, among other things, comprises patients with fairly rare monoallergies, close correlation was found between RAST and MAST, although the test allergens were not the same.…”

Section: Discussionmentioning

confidence: 99%

Comparative evaluation of RAST and MAST‐CLA for six allergens for the diagnosis of inhalant allergic disease in 232 patients

Scolozzi

Vicentini

Boccafogli

et al. 1992

Clin Experimental Allergy

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The aim of this study was to compare a recent multiple allergosorbent chemiluminescent assay (MAST-CLA) with the RAST for the diagnosis of inhalant allergic disease in 232 patients with rhinitis and/or bronchial asthma. As judged by concordance of clinical history, skin prick tests to a range of six allergens common to our geographic area, and by nasal provocation tests, 70 patients were non-allergic and 162 allergic: 70 to grasses, 46 to mites, four to mugwort, eight to pellitory, and 34 were sensitive to several allergens. In our patient sample that, among other things, comprises subjects with fairly rare monoallergies, MAST-CLA testing gave results which closely corresponded to positive RAST for the allergens studied, and demonstrated a close correlation with the diagnosis of inhalant-specific allergy. Our results showed that, for overall allergens, MAST-CLA was lightly less sensitive and more specific than RAST (the two in vitro tests gave an identical overall efficiency).

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Comparison of MAST With Radioallergosorbent and Skin Tests for Diagnosis of Allergy in Children

Cited by 16 publications

References 9 publications

Relationship between skin‐prick tests, the multiple allergosorbent test and symptoms of allergic disease

Relationship between skin‐prick tests, the multiple allergosorbent test and symptoms of allergic disease

Multiple Allergen Simultaneous Test-Immunoblot Assay for Immunoglobulin E Detection in Patients with Isolated Allergic Conjunctivitis

Comparative evaluation of RAST and MAST‐CLA for six allergens for the diagnosis of inhalant allergic disease in 232 patients

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