Multiple Allergen Simultaneous Test-Immunoblot Assay for Immunoglobulin E Detection in Patients with Isolated Allergic Conjunctivitis

Abstract

We aimed to investigate the immunoglobulin E (IgE) detection rate and allergen patterns in patients with isolated allergic conjunctivitis using the multiple allergen simultaneous test (MAST)-immunoblot assay. A total of 120 patients with allergic conjunctivitis and no associated rhinitis, asthma, or dermatitis underwent the MAST-immunoblot assay to measure serum total IgE (TIgE) and serum specific IgE (SIgE) against 57 allergens. Patients were classified into subgroups based on the season when the eye symptoms were exacerbated, and TIgE and SIgE positivity. Differences between sex and age groups were also analyzed. Of the 120 patients, 57.5% (69 patients) and 69.2% (83 patients) were positive for TIgE (≥100 IU/mL) and SIgE (≥0.7 IU/mL), respectively. The allergens that most frequently triggered sensitization in the study population were Dermatophagoides farinae, Dermatophagoides pteronyssinus, Tyrophagus putrescentiae, Alternaria, and house dust. House dust mites, such as D. farinae and D. pteronyssinus, showed the highest detection rates regardless of the season. Men had a higher positive rate for TIgE than women, whereas a higher rate of sensitization, detected as SIgE positivity, was seen in younger patients. In conclusion, MAST-immunoblot assay can detect sensitizing allergens in patients with isolated allergic conjunctivitis.

Association of GATA3 gene polymorphism with allergic conjunctivitis and allergic rhinitis

“…In the present study, a1lergic conjunctivitis patients' age ranged from 10 to 55 which was in accordance with Han et al 11 who reported that age range was from 4 to 78 years. Moreover, the age range in our study for a1lergic rhinitis patients was 12 to 59 which was comparable to Shirkani et al 12 who reported that age range was from 15 to 60 years and to Zhang et al 16 in which the age ranged between 8-61 years.…”

Topical ketotifen treatment for allergic conjunctivitis: a systematic review and Meta-analysis

“…Allergic conjunctivitis is caused by type 1 immunoglobulin E -mediated hypersensitivity reaction. Its biological mechanism is that allergen particles bind and cross connect immunoglobulin E molecules which are attached to the tissue mast cells, resulting in these cells degranulation and release histamines and inflammatory mediators into the tissue [3] . At present, the topical therapeutic drugs for allergic conjunctivitis include antihistamines, cyclosporine ophthalmic solution, mast cell stabilizers, and eosinophil deactivators [4] .…”