Comparative evaluation of RAST and MAST‐CLA for six allergens for the diagnosis of inhalant allergic disease in 232 patients

Scolozzi, R; Vicentini, L.; Boccafogli, A; Camerani, Annalisa; Pradella, R.; Cavallini, Anna; Bagni, B

doi:10.1111/j.1365-2222.1992.tb03077.x

Cited by 8 publications

(7 citation statements)

References 11 publications

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“…A significant association between the severity score of AD and the concentration of allergen-specific IgE to dust mites and cats was demonstrated by Schafer and collegues (21). Many studies have demonstrated that the MAST-CLA ® allergen test system provides an equal efficiency compared with skin test and radioallergosorbent test (RAST) in the evaluation of sensitized allergens in atopic patients (22)(23)(24). AD patients with concomitant perennial allergic rhinitis are more likely to have specific antibodies to mites, housedust, cockroaches, and feathers ( Table 2).…”

Section: Discussionmentioning

confidence: 99%

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Correlation of Serum Total IgE, Eosinophil Granule Cationic Proteins, Sensitized Allergens and Family Aggregation in Atopic Dermatitis Patients with or without Rhinitis

Lee

Chuang

Shih

et al. 2004

The Journal of Dermatology

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Atopic dermatitis (AD) is a complex disease with both a genetic background and environmental interactions. Although multiple linkage-analyses about AD have been studied, there have been only a few family aggregation tests of AD or perennial allergic rhinitis (AR) to date. The association of allergen-specific IgE in AD and atopic dermatitis with allergic perennial rhinitis (ADR) have also been seldom discussed. The purpose of this study was to evaluate family aggregation and assess allergen-specific IgE in patients with AD and ADR. We also planned to investigate the effect of family history of AD on the prevalence of allergen-specific antibodies. The serum levels of IgE, eosinophil cationic protein (ECP) and major basic protein (MBP) were measured and compared in patients with AD and those with ADR. Proportional analysis compared allergen-specific IgE between AD and ADR. The family aggregation was conducted to estimate the odds ratio for various atopic diseases in different family members. Total IgE and allergen-specific antibodies in serum were compared between those patients who had AD with AR and those without. The result revealed that allergic rhinitis is the most common concomitant atopic disease associated with AD. The ADR group was more likely to have serum mite-, cockroach-, and feather-specific IgE. The positive rates for wheat, peanut and soybean were higher in those AD without rhinitis. In the family aggregation of AD, the odds ratio for siblings was higher than for parents, the ratios for brother and sister were 9.91 and 8.75, respectively. However, the odds ratio for parents of ADR was higher than siblings; the ratios for father and mother of ADR were 8.22 and 2.94, respectively. AD patients with family histories of AD were more likely to have mite-, soybean-, and peanut-specific antibodies in their serum. We concluded that aeroallergens are the most important allergens aggravating atopic diseases in Taiwan. Food plays an important role in the pathogenesis of AD. Measurement of serum total IgE combined with the MAST-CLA test could be helpful in the diagnosis of atopic diseases. The differential aggregation tendency for AD and ADR implicated the complexity of the gene-environment interaction in these atopic diseases.

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Section: Discussionmentioning

confidence: 99%

“…MBP and ECP are major cytotoxic proteins released from activated eosinophils in inflammation (20)(21)(22)(23)(24)(25)(26). Both of them may reflect the disease activity of atopic diseases through damage to cells.…”

Section: Discussionmentioning

confidence: 99%

Correlation of Serum Total IgE, Eosinophil Granule Cationic Proteins, Sensitized Allergens and Family Aggregation in Atopic Dermatitis Patients with or without Rhinitis

Lee

Chuang

Shih

et al. 2004

The Journal of Dermatology

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“…In this study, the presence of allergy was defined using the MAST-CLA system, which is used to detect allergen-specific IgE antibodies using enzyme-linked anti-human IgE and a chemiluminescent assay, as reported previously [ 23 ]. This test avoids the use of radioactive reagents used in other in vitro tests and it permits the simultaneous quantitative measurement of specific IgE directed against 35 different allergens [ 24 ]. MAST-CLA sensitivity, specificity, and precision equal those of RAST and skin prick tests [ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Detection of bacteria in middle ear effusions based on the presence of allergy: Does allergy augment bacterial infection in the middle ear?

Kim

Chang

et al. 2015

Journal of Otolaryngology - Head & Neck Surgery

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Background: Bacterial infection, Eustachian tube dysfunction, allergies, and immunologic factors are major causes of otitis media with effusion (OME). However, the exact pathogenesis of OME is still unclear. This study evaluated whether allergy influences bacterial growth in middle ear effusions. Materials: Fifty-four samples were obtained from OME patients 3-10 years of age who underwent ventilation tube insertion and were divided into two groups based on the presence of allergy as determined using the multiple allergosorbent test (MAST). Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis bacterial DNA in the middle ear effusions was analyzed using polymerase chain reaction. Overall detection rates and those for each species were compared between the two groups. Results: Of the 54 middle ear effusion samples, 38 (70.4 %) contained bacterial DNA and 14 (36.8 %) of these contained DNA from multiple species. S. pneumoniae was detected in 27 samples (50 %), H. influenzae in 17 samples (31.4 %), and M. catarrhalis in 9 samples (16.6 %). There was no significant difference in the bacterial detection rates between the middle ear effusions of the MAST-positive and MAST-negative groups. Conclusion: The rate of bacteria detection in middle ear effusions did not differ between allergic and non-allergic children.

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“…, are commonly used, and have evidenced good agreement rates of results with the skin prick test (Agata et al, 1993;Finnery et al, 1989;Orgino et al, 1993;Scolozzi et al, 1989;Scolozzi et al, 1992;Tang and Wu, 1989;Tang et al, 1994). MAST-CLA has the advantage that it can test for 35 common allergens simultaneously, and thus it is suitable as a screening test.…”

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confidence: 99%

Validity of MAST-CLA for diagnosis of arthropod allergy using receiver operating characteristic (ROC) analysis

et al. 2007

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Comparative evaluation of RAST and MAST‐CLA for six allergens for the diagnosis of inhalant allergic disease in 232 patients

Cited by 8 publications

References 11 publications

Correlation of Serum Total IgE, Eosinophil Granule Cationic Proteins, Sensitized Allergens and Family Aggregation in Atopic Dermatitis Patients with or without Rhinitis

Correlation of Serum Total IgE, Eosinophil Granule Cationic Proteins, Sensitized Allergens and Family Aggregation in Atopic Dermatitis Patients with or without Rhinitis

Detection of bacteria in middle ear effusions based on the presence of allergy: Does allergy augment bacterial infection in the middle ear?

Validity of MAST-CLA for diagnosis of arthropod allergy using receiver operating characteristic (ROC) analysis

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