Comparison of Mast Cells and Inflammatory Cells within Periapical Lesions and Comparison of Degranulated Mast Cells Between Fibrous and Inflamed Area in Radicular Cysts: An Immunohistochemical Study

Shiromany, Aseem; Sood, Rahul; Akifuddin, Syed; Sidhu, Gagandeep Kaur; Khan, Nadia; Singla, Kapil

doi:10.7860/jcdr/2014/11623.5340

Cited by 6 publications

(11 citation statements)

References 11 publications

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“…Similar to the results of other studies (Marcal et al 2010, Costa Neto et al 2015, the present study revealed a larger number of tryptase-positive mast cells in PGs compared to RCs. However, some studies demonstrated different findings (Rodini et al 2004, Drazi c et al 2010, Lima et al 2011, Fonseca-Silva et al 2012, Shiromany et al 2014. Based on the present immunohistological data (Costa Neto et al 2015), these divergences between results may be attributed mainly to differences in morphometric analysis and in the sample size used in each study (Fonseca-Silva et al 2012).…”

Section: Discussionmentioning

confidence: 65%

“…It has become clear that mast cells are implicated in inflammatory processes in the cyst capsule and may be involved in fibrous tissue formation, due to the release of several mediators during its degranulation (IgE antibodies, histamine, serotonin, heparin and proteases) (Drazi c et al 2010). These mediators play an important role in connective tissue remodelling, fibrosis, chronicity (by TNF-a release) and expansion of periapical lesions (Shiromany et al 2014). As RRCs are usually longstanding pathoses with reduced inflammation, the findings of this study suggest that mast cells are recruited to cystic fibrous walls to assist in maintenance, persistence and growth of this lesion.…”

Section: Discussionmentioning

confidence: 67%

“…These mediators play an important role in connective tissue remodelling, fibrosis, chronicity (by TNF‐α release) and expansion of periapical lesions (Shiromany et al . ). As RRCs are usually long‐standing pathoses with reduced inflammation, the findings of this study suggest that mast cells are recruited to cystic fibrous walls to assist in maintenance, persistence and growth of this lesion.…”

Section: Discussionmentioning

confidence: 97%

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Analysis of tryptase‐positive mast cells and immunoexpression of MMP‐9 and MMP‐13 in periapical lesions

et al. 2016

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Section: Discussionmentioning

confidence: 65%

Section: Discussionmentioning

confidence: 67%

Section: Discussionmentioning

confidence: 97%

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Analysis of tryptase‐positive mast cells and immunoexpression of MMP‐9 and MMP‐13 in periapical lesions

et al. 2016

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“…By IHC, the objective way to demonstrate a mast cell phenotype is the cytoplasmic presence of the constituents of its secretory granules, such as chymase and/or tryptase [5][6][7][8]. Tryptase and chymase are both used in the detection of normal [8,9] or neoplastic [10][11][12] conditions, and chymase activity is closely correlated with inflammatory diseases progression [8]. Atopic dermatitis in dogs is a chronic inflammatory skin disease involving abnormalities in skin barrier function and cutaneous inflammation [13].…”

Section: Introductionmentioning

confidence: 99%

A Double Histochemical/Immunohistochemical Staining for the Identification of Canine Mast Cells in Light Microscopy

Migliaccio

Sarli

Silva

et al. 2021

Veterinary Sciences

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Immunohistochemistry (IHC) is a widely used technique in diagnostic pathology, but the simultaneous analysis of more than one antibody at a time with different chromogens is rather complex, time-consuming, and quite expensive. In order to facilitate the identification of mast cells (MCs) during immunohistochemical analysis of membrane and/or nuclear markers, we propose a new staining method that includes the association of IHC and toluidine blue as a counterstain. To achieve this goal, we tested c-kit, Ki67, and cannabinoid receptor 2 on several cases of cutaneous canine mast cell tumors (MCTs), cutaneous mastocytosis, and atopic dermatitis. The results obtained show how this double staining technique, although limited to non-cytoplasmic markers and of little use in poorly differentiated MCTs in which MC metachromasia is hard to see, can be used during the evaluation of nuclear and/or membranous immunohistochemical markers in all canine cutaneous disorders, especially if characterized by the presence of a low number of MCs. It can help to evaluate those MCTs in which neoplastic MCs must be clearly distinguished from inflammatory cells that can infiltrate the tumor itself, in facilitating the calculation of the Ki67 index. Moreover, it can be used to study the expression of new markers in both animal and human tissues containing MCs and in MC disorders.

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“…The present study is undertaken to compare and quantify MCs in different zones of periapical granuloma and cysts and thus to identify their role in the pathogenesis of the lesion. [ 6 ]…”

Section: Introductionmentioning

confidence: 99%

Quantification and distribution of mast cells in oral periapical inflammatory lesions

Choudhary¹,

Kesarwani

Koppula

et al. 2021

J Conserv Dent

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Background: Periapical granuloma and periapical cysts develop as a sequela of inflammation of dental pulp, usually a consequence of an infected root canal. Mast cells (MCs) have most frequently been implicated in the pathophysiology of periapical lesions such as periapical granulomas and cysts. Aims: The present study was undertaken to emphasize the presence and quantification of MCs in periapical lesions (granulomas and cysts) using toluidine blue stain, with an aim to elucidate their role in the pathogenesis of these lesions. Materials and Methods: The study comprised a total of 55 previously diagnosed cases (30 cases of radicular cyst and 25 cases of periapical granuloma) selected from the departmental archives. The MCs were quantified in all the sections in the three zones, namely the epithelial, subepithelial, and deeper connective tissue zones. The results were sent for statistical analysis. Statistical Analysis: Wilcoxon paired t -test and Mann–Whitney U test were applied for intra- and intergroup comparison MCs in different zones of periapical granulomas and radicular cyst, respectively. Results: A statistically significant difference was observed between the two lesions in all the three zones, i.e., epithelial, subepithelial, and in deeper connective tissue zone with P = 0.001. Conclusion: Although MCs were present in all the three zones of both periapical cysts and granuloma, they were predominantly observed in radicular cysts than in periapical granuloma. This suggests a significant role of MC in initiation, development, and progression of these periapical lesions.

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Comparison of Mast Cells and Inflammatory Cells within Periapical Lesions and Comparison of Degranulated Mast Cells Between Fibrous and Inflamed Area in Radicular Cysts: An Immunohistochemical Study

Cited by 6 publications

References 11 publications

Analysis of tryptase‐positive mast cells and immunoexpression of MMP‐9 and MMP‐13 in periapical lesions

Analysis of tryptase‐positive mast cells and immunoexpression of MMP‐9 and MMP‐13 in periapical lesions

A Double Histochemical/Immunohistochemical Staining for the Identification of Canine Mast Cells in Light Microscopy

Quantification and distribution of mast cells in oral periapical inflammatory lesions

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