Analysis of tryptase‐positive mast cells and immunoexpression of <scp>MMP</scp>‐9 and <scp>MMP</scp>‐13 in periapical lesions

Andrade, Ana Luiza Dias Leite de; Santos, E. M.; Carmo, Andréia Ferreira Do; Freitas, Roseana A.; Galvão, Hébel Cavalcanti

doi:10.1111/iej.12638

Cited by 20 publications

(31 citation statements)

References 33 publications

(86 reference statements)

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“…A significantly higher number of PGs had a Grade 3 inflammatory infiltrates, 27-fold higher than in RCs. These results are similar to those reported by Andrade et al (2016) 18 , who detected intense inflammatory infiltrates in both lesions, explaining their inflammatory nature.…”

Section: Discussionsupporting

confidence: 93%

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Macrophages subpopulations in chronic periapical lesions according to clinical and morphological aspects

et al. 2019

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The aim of this study was to evaluate macrophage M1 and M2 subpopulations in radicular cysts (RCs) and periapical granulomas (PGs) and relate them to clinical and morphological aspects. M1 macrophages were evaluated by the percentage of CD68 immunostaining associated with the inflammatory cytokine TNF-α, and M2 macrophages, by its specific CD163 antibody. The CD68 + / CD163 + ratio was adopted to distinguish between the two macrophage subpopulations. Clinical, radiographic, symptomatology, treatment, and morphological parameters of lesions were collected and a significance level of p = 0.05 was adopted for statistical analysis. The results showed that the CD68 + /CD163 + ratio was higher in the RCs (median = 1.22, p = 0.002), and the highest TNF-α immunostaining scores were found in RCs (p = 0.018); in PGs, the CD68 + /CD163 + ratio was lower and associated with a greater CD163 + immunostaining (median = 1.02, p <0.001). The TNF-α in cyst epithelium had a score of 3 in 10 cases and predominance of M1 macrophages by CD68 + /CD163 + (median = 2.23). In addition, CD68 + cells had higher percentage of immunostaining in smaller RCs (p = 0.034). Our findings suggest that increased CD68 immunostaining associated with TNF-α cytokine in RCs results in a greater differentiation of the M1 phenotype. The higher CD163 immunostaining in PGs results in greater differentiation of the M2 phenotype. Therefore, the inflammatory state promoted by M1 macrophages is related to growth and progression of RCs; on the other hand, the immunomodulatory state of M2 macrophages is related to maintenance of PGs.

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Section: Discussionsupporting

confidence: 93%

“…Manufacturer, clone, antigen retrieval, dilution, and incubation period of primary antibodies. 18 , as follows: score 1 (≤25% positive cells), score 2 (>25% and ≤50% positive cells), score 3 (>50% and ≤75 % positive cells), and score 4 (>75% of positive cells).…”

Section: Immunohistochemical Analysesmentioning

confidence: 99%

Macrophages subpopulations in chronic periapical lesions according to clinical and morphological aspects

et al. 2019

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“…In addition, no differences were established in the present study between the epithelial thickness of radicular cyst lining and the immunoexpression of TGF-β1 and MMP-9, as corroborated by other authors. 25,26 In this research, the TGF-β1 expression revealed differences between periapical granulomas and radicular cysts, thus corroborating the observation by Marçal et al 27 and Teixeira-Salum et al 28 . Although the data could not confirm any significant association between symptoms and TGF-β1 expression, their descriptive analysis showed that the majority of granulomas were asymptomatic and presented a greater number of cases with higher TGF-β1 scores (score 2).…”

Section: Discussionsupporting

confidence: 89%

“…These results are corroborated by Faustino et al 30 and Andrade et al 26 , who showed that the MMP-9 immunoexpression was no different between symptomatology and grade of inflammation among periapical granulomas, radicular cysts and residual radicular cysts.…”

Section: Discussionsupporting

confidence: 73%

“…Notwithstanding, an immunohistochemistry assay by Carneiro et al 29 found that the non-epithelialized periapical lesions exhibited higher MMP-9-positive cells, but no significant differences were observed among these lesions. Andrade et al 26 also evidenced a higher expression of MMP-9 in granulomas than in radicular and residual radicular cysts. These data suggest that MMP-9 presents a similar remodeling activity of extracellular matrix in periapical lesions.…”

Section: Discussionmentioning

confidence: 90%

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Immunohistochemical expression of TGF-β1 and MMP-9 in periapical lesions

et al. 2017

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The objective of this study was to evaluate the expression of matrix metalloproteinase 9 (MMP-9) and transforming growth factor beta (TGF-β1) in periapical lesion samples correlated with the intensity of the inflammatory infiltrate and thickness of the epithelial lining. Forty-five cases of periapical lesions (23 periapical granulomas and 22 radicular cysts) were subjected to morphological and immunohistochemical analyses using anti-MMP-9 and anti-TGF-β1 antibodies. The data were analyzed using the following tests: non-parametric Mann-Whitney, chi-square, Fisher's exact test and Spearman's correlation test (P<0.05). Analysis of inflammatory infiltrate revealed that 78% of periapical granulomas presented infiltrate grade III, in contrast with 32% of radicular cysts (P<0.001). Morphological evaluation of the epithelial thickness in radicular cysts revealed the presence of atrophic epithelium in 86% of the cysts. The immunostaining of MMP-9 was score 2 in 67% of the granulomas and 77% of the cysts. Both lesions were predominantly score 1 for TGF-β1. Significant differences were confirmed between the expression scores of TGF-β1 and MMP-9 in periapical granulomas (p = 0.004) and in radicular cysts (p < 0.001). Expression of TGF-β1 was different for periapical granulomas and radicular cysts. This immunoregulatory cytokine seems more representative in asymptomatic lesions. The extracellular matrix remodeling process dependent on MMP-9 seems to be similar for both periapical granulomas and radicular cysts. TGF-β1 and MMP-9 may play an important role in the maintenance of periapical lesions.

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2022

Shear's Cysts of the Oral and Maxillofacial Regions

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Analysis of tryptase‐positive mast cells and immunoexpression of MMP‐9 and MMP‐13 in periapical lesions

Abstract: A larger number of tryptase-positive mast cells and greater enzymatic activity of MMP-9 and MMP-13 were found in PGs compared to RCs and RRCs. These findings are a characteristic of the dynamics of periapical diseases.

Cited by 20 publications

References 33 publications

Macrophages subpopulations in chronic periapical lesions according to clinical and morphological aspects

Macrophages subpopulations in chronic periapical lesions according to clinical and morphological aspects

Immunohistochemical expression of TGF-β1 and MMP-9 in periapical lesions

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