Quantification and distribution of mast cells in oral periapical inflammatory lesions

Choudhary, Anand; Kesarwani, Pallavi; Koppula, Srikrishna; Verma, Saumya; Saumya, Srishti; Srivastava, Parul

doi:10.4103/jcd.jcd_505_21

Cited by 5 publications

(1 citation statement)

References 20 publications

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“…PC, synonymously known as radicular cysts, comprise 35%–87% of all odontogenic cysts and arise from the epithelial remnants of periodontal ligament following necrosis of the pulp. [ 11 12 13 ] Little information is available regarding PC and granuloma from the Indian cohort;[ 8 9 10 ] thus, the present study was conducted to demonstrate clinicodemographic and histopathological features of these common inflammatory periapical lesions.…”

Section: Discussionmentioning

confidence: 99%

Clinicopathological comparison of periapical cyst and periapical granuloma in a cohort of Tamil population

Immanuel,

Pandiar,

Krishnan

2024

Journal of Conservative Dentistry and Endodontics

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Aim: The aim of this study was to present and analyze detailed clinicopathological data of periapical cysts (PCs) and periapical granuloma (PG) in a cohort of 135 cases from the South Indian Population. Methodology: The present study included 135 cases of PC and PG out of 2696 biopsies submitted over 3 years. The clinicodemographic data which included age, gender, location, radiographic appearance, and treatment were collected along with the histopathological examination of the biopsied specimen. Data were entered in a Microsoft Excel spreadsheet, 2021, and analyzed using SPSS software ver. 26. Results: There were 71 cases of PG and 64 cases of PC. The mean age of occurrence in PG was slightly lower than cases in PC. Irrespective of the group, there was a clear male preponderance, and maxillary permanent central incisors were most commonly affected. However, no significant difference was noted. Radiographically, PC significantly showed more well-defined corticated radiolucent lesions compared to PG where most cases were ill-defined (69.01%). Histologically, all cases showed classic features for diagnosis with additional histological characteristics which may aid in diagnosis. Conclusion: PG was more common than PC. There was a predilection for the male gender in both lesions. The actual incidence of these lesions would be actually high, as some cases are lost to private practitioners, and not all the lesions are submitted for histopathological examination.

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Section: Discussionmentioning

confidence: 99%

Clinicopathological comparison of periapical cyst and periapical granuloma in a cohort of Tamil population

Immanuel,

Pandiar,

Krishnan

2024

Journal of Conservative Dentistry and Endodontics

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Quantification of immune and inflammatory response cells and beta-defensin-3 (hBD-3) expression in radicular cysts of primary and permanent teeth

Bertasso,

Esquiche,

Jorge

et al. 2023

Preprint

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Objectives To quantify mast cells, mature and immature plasmacytoid dendritic cells, mature and immature Tregs, cytotoxic T lymphocytes, and B lymphocytes, and hBD-3 expression in radicular cysts of primary and permanent teeth and to evaluate the relationship between radiographic lesion size and the intensity of hBD-3 staining. Material and Methods 19 radicular cysts of primary teeth and 17 of permanent teeth were subjected to immunohistochemical analysis for quantification of immune and inflammatory response cells and for evaluation of hBD-3 and its relationship with the radiographic lesion size. The results were analyzed using D'Agostino & Pearson, Mann–Whitney, t-test, Kruskal–Wallis and Dunn's post-test, at 5% significance level. Results All the evaluated cell types were detected in all radicular cysts. Cysts of primary teeth showed higher expression of plasmacytoid dendritic cells (mature and immature), B lymphocytes, and T lymphocytes (p < 0.05), whereas those in permanent teeth showed higher expression of T lymphocytes, immature plasmacytoid dendritic cells, cytotoxic T lymphocytes, and B lymphocytes (p < 0.05). hBD-3 was expressed in primary teeth similarly in the capsule and epithelium (p > 0.05), whereas in permanent teeth the expression was higher in the capsule (p < 0.05). In primary teeth, hBD-3 expression in small lesions was higher than in large lesions (p < 0.05). Conclusion Immature plasmacytoid dendritic cells were the predominant cells in radicular cysts in primary teeth whereas T lymphocytes were more abundant in permanent teeth. hBD-3 was expressed in the epithelium/capsule in both primary and permanent teeth. In primary teeth, a direct relationship was observed between hBD-3 expression and radicular cyst size. Clinical Relevance The present study should provide a better understanding of radicular cysts, which would be useful for devising targeted treatment techniques and immunologically active materials for endodontic use.

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Human Periapical Odontogenic Granulomas: Aspects of Microvessel Density (MVD), Heterogeneity of Blood Vessels and Mast Cells Density (MCD)

Roi,

Negruțiu,

Roi

et al. 2023

Biomedicines

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Periapical odontogenic granulomas are among the most encountered pathology that involve the alveolar bone, with severe consequences such as bone resorption, the presence of inflammatory infiltrate and the formation of abnormal vascularization. The present study aimed to quantify the existence of the microvessel density (MVD), mast cell density (MCD) and heterogeneity of the encountered blood vessels. A total of 37 patients diagnosed with odontogenic periapical granulomas were included, and the gender distribution, age and localization of the pathological lesions was assessed. After the surgical removal of the periapical odontogenic granuloma, the collected tissue was fixed in 10% buffered formalin. Primary processing, morphological analysis and immunohistochemical staining was performed in order to characterize the altered tissue. The results outlined the presence of a high number of mast cells, especially in the area of the inflamed tissue; the high heterogeneity of the blood vessels; and increased MVD with positive CD34. The conclusions of the study focus on the key role of the mast cells and their implication in the initiation and development of the angiogenesis process, triggering the inflammatory response of the host. Nevertheless, periapical odontogenic granulomas develop as an inflammatory response to the interaction between the host’s immune system and microbial invasion.

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Quantification and distribution of mast cells in oral periapical inflammatory lesions

Cited by 5 publications

References 20 publications

Clinicopathological comparison of periapical cyst and periapical granuloma in a cohort of Tamil population

Clinicopathological comparison of periapical cyst and periapical granuloma in a cohort of Tamil population

Quantification of immune and inflammatory response cells and beta-defensin-3 (hBD-3) expression in radicular cysts of primary and permanent teeth

Human Periapical Odontogenic Granulomas: Aspects of Microvessel Density (MVD), Heterogeneity of Blood Vessels and Mast Cells Density (MCD)

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