Comparison of Lipids and Lipopolysaccharide from the Bacillary and L Forms of
            <i>Proteus</i>
            P18

Nesbitt, John A.; Lennarz, William J.

doi:10.1128/jb.89.4.1020-1025.1965

Cited by 55 publications

(17 citation statements)

References 22 publications

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“…As was found already in the 1960s, fatty acids with a cyclopropane ring are very susceptible to acidic conditions that cause cleavage of the ring. This was documented in 1965 by Nesbitt and Lennarz [33] who therefore saponified cells of the genus Proteus by 10% NaOH and methylated free FA by diazomethane. This approach, which was used also by other authors [34], avoided the cleavage of the cyclopropane ring.…”

Section: Discussionmentioning

confidence: 80%

Hydrophilic Interaction Liquid Chromatography: ESI–MS/MS of Plasmalogen Phospholipids from Pectinatus Bacterium

Řezanka

Siříšťová

Matoulková

et al. 2011

Lipids

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Liquid chromatography-electrospray tandem mass spectrometry (LC/ESI-MS/MS) was used to analyze phospholipids from three species of the anaerobic beer-spoilage bacterial genus Pectinatus. Analysis of total lipids by HILIC (Hydrophilic Interaction Liquid Chromatography) column succeeded in separating diacyl- and plasmalogen phospholipids. Plasmalogens were then analyzed by means of the ESI-MS/MS and more than 220 molecular species of four classes of plasmalogens (PlsCho (choline plasmalogen), PlsEtn (ethanolamine plasmalogen), PlsGro (glycerol plasmalogen), and PlsSer (serine plasmalogen)) were identified. Major molecular species were c-p19:0/15:0 PlsEtn and PlsSer, which accounted for more than 4% of the total lipids.

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Section: Discussionmentioning

confidence: 80%

Hydrophilic Interaction Liquid Chromatography: ESI–MS/MS of Plasmalogen Phospholipids from Pectinatus Bacterium

Řezanka

Siříšťová

Matoulková

et al. 2011

Lipids

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“…The fatty acids were obtained from the glycerides by alkaline hydrolysis (6), and the methyl esters were prepared for gas liquid chromatographic (GLC) analysis by the method of aThe fatty acids were identified as methyl esters by comparing their retention time with that of authentic samples. The composition of the fatty acids was calculated on the basis of their gas chromatographic areas by the triangulation procedure.…”

Section: Preparation Of Derivativesmentioning

confidence: 99%

Distribution of fatty acids during germination of cottonseeds

Joshi

Doctor

1975

Lipids

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Gas chromatographic determination of the fatty acids in the seeds of cottonseed (Gossypium sp.) showed linoleic, palmitic, oleic, and stearic acids, with linoleic acid being the major component. Changes in the composition of fatty acids during various stages of germination were measured in the cotyledons and in the roots. A decrease in the content of all the fatty acids and an 8-fold increase in the moisture content of the cotyledons were observed during the 10 days of germination. There were no significant changes in the fatty acid contents of the roots with the exception of those in linoleic acid which increased by 50 percent during 4-10 days of germination. The possible significance of these changes is discussed.

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“…Protoplast lysis in the presence and absence of EDTA. Mutant CMK 33 and wild-type cells were grown in 60 ml of medium C containing14 ,Ci of 32P1. Lysis curve determinations were performed as described in…”

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confidence: 99%

Procedure for the Isolation of Mutants of Bacillus subtilis with Defective Cytoplasmic Membranes

1973

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A procedure has been devised to isolate mutants of Bacillus subtilis with structurally defective membranes. The procedure used to screen for the mutants involved comparison of the stability of protoplasts of the mutant with those of the wild type in a medium of sufficient osmotic strength to stabilize wild-type protoplasts. Mutagenized cells were grown as clones on agar plates, and then replicated onto plates containing 0.5 M lactose, which is sufficient to stabilize wild-type protoplasts. The colonies on the lactose-containing plates were then treated with lysozyme to convert the cells to protoplasts. Colonies of wild-type protoplasts remained opaque; however, colonies of mutant protoplasts lysed and became clear. Twenty-nine osmotically fragile mutants were isolated in this manner; the membranes of several mutants were found to contain alterations in the composition of their proteins or lipids.Public Health. This strain is a derivative of B. subtilis 168 and is auxotrophic for threonine, leucine, and methionine (18).Growth media. Medium A consisted of 1% peptone and 1% yeast extract (Difco Laboratories), adjusted to pH 7.0 with 2 N NaOH. Medium B was the same as A except that it also contained 0.5 M lactose (Pfanstiehl Laboratories, Inc.). Medium C was the same as A except that it also contained 0.5% NaCl and 0.04% Na2HPO4. Medium D was a chemically defined medium containing (in 1 liter): 20 mg each of L-

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Comparison of Lipids and Lipopolysaccharide from the Bacillary and L Forms of Proteus P18

Cited by 55 publications

References 22 publications

Hydrophilic Interaction Liquid Chromatography: ESI–MS/MS of Plasmalogen Phospholipids from Pectinatus Bacterium

Hydrophilic Interaction Liquid Chromatography: ESI–MS/MS of Plasmalogen Phospholipids from Pectinatus Bacterium

Distribution of fatty acids during germination of cottonseeds

Procedure for the Isolation of Mutants of Bacillus subtilis with Defective Cytoplasmic Membranes

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