on the L fornm of Proteuts P18 and the parent bacterium grown in a defined medium showed that the L form contained 1.5 times as much extractable lipid (dry weight) as the bacillary form. The composition of the lipids extractable by chloroform-methanol was quite similar in the two bacterial forms. The occurrence of mvristate and ,-hydroxymyristate in the bound, nonextractable lipid was found to be a reflection of the presence of lipopolysaceharide (LPS) in each organism. The bacillary organism contains three to four times as mnuch LPS as the L form. The LPS isolated from both organisms contains heptose, hexosamine, phosphorus, 3-deoxyoctulosonate, glucose, galactose, and fatty acids.
Coumarin-induced necrosis of the skin and subcutaneous tissue is an uncommon but well recognized complication of anticoagulant therapy. Although any area of skin may be involved necrosis of the penis is rare. We report a case of penile necrosis associated with coumarin therapy and review the literature.
Using microsurgical techniques, the perineurial sheath was stripped off the sciatic nerves of rats over a 0.5 cm length at a point where the nerve consists of a single fascicle. The nerves were excised 0 to 84 days after the injury, and were examined in semi-thin transverse section. A new sheath, closely resembling normal perineurium, became organized during the first 10 days; it appeared uniformly over the length of the injured segment. The new perineurial sheath was probably formed by endoneurial fibroblasts migrating from within the fascicle. In undamaged specimens, the asons immediately beneath the excised perineurium underwent no degenerative changes.
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