COMPARISON OF INHIBITORY ACTIVITY AGAINST THE Α-Glucosidase ENZYMES IN THE EXTRACTS AND FRACTIONS FROM LEAVES OF THE GARCINIA KYDIA ROXBURGH

Martha, Septiani; Elya, Berna; Hanafi, Muhammad

doi:10.22159/ajpcr.2017.v10i7.18529

Cited by 3 publications

(2 citation statements)

References 15 publications

(18 reference statements)

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“…Likewise, F-EtOAc leaf fractions had α-glucosidase inhibitory activity, similar to other species in the Clusiaceae family 29,[32][33][34] . Flavonoids and xanthones have been isolated from some species of the Clusiaceae family [34][35][36][37][38] . There is a strong relationship between phenolic compounds from natural sources and α-glucosidase inhibition [39][40][41][42][43] .…”

Section: Discussionmentioning

confidence: 99%

The Antioxidant and Hypoglycemic Properties and Phytochemical Profile of Clusia latipes Extracts

Silva-Rivas¹,

Bailón-Moscoso²,

Cartuche³

et al. 2020

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Introduction: The prevalence of diabetes has increased more rapidly in low and middleincome countries than in high-income countries. Type 2 diabetes mellitus (DM2), which is the most common form of diabetes, is caused by the inefficient use of insulin in the body and is characterized by disrupted insulin action or secretion. Also, oxidative stress plays an important role in the development of disease. The goal of this study is to identify the antioxidant and hypoglycemic properties of Clusia latipes, an endemic species of Central and South America. Methods: The antioxidant and hypoglycemic capacity of the extracts (hexane, ethyl acetate, and methanol) of the leaves and stems of Clusia latipes were evaluated. From the most potent extract, the phytochemical study was carried out and fractionated. Antioxidant activity was measured using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), while hypoglycemic capacity was measured by alpha-glucosidase inhibition. Results: The extracts with the highest antioxidant capacity are the extracts with the highest α-glucosidase inhibition activity. Inhibitory activity increased in samples extracted with medium polar (ethyl acetate) and polar (methanol) solvents. Phytochemical screening of these extracts revealed the presence of alkaloids, carbohydrates, flavonoids/xanthones, quinones, saponins, and tannins. The highest α-glucosidase inhibitory activity was detected in the ethyl acetate fraction obtained from leaf methanol extract, with a half-maximal inhibitory concentration (IC 50 ) value of 0.90 μg/ml. The major constituent isolated from the same fraction was isoquercitrin.

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Section: Discussionmentioning

confidence: 99%

The Antioxidant and Hypoglycemic Properties and Phytochemical Profile of Clusia latipes Extracts

Silva-Rivas¹,

Bailón-Moscoso²,

Cartuche³

et al. 2020

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“…for the fractionation process of several extracts of natural products such as the fractionation of Garcinia lateriflora, Pluchea odorata, G. kydia, Senna ocidentalis, and G. hombroniana [31][32][33][34] . The fractionation process using column chromatography on methanol extract as the most active extract as an antioxidant resulted in eight fractions, as shown in Table II.…”

Section: Fractionation Yield and Antioxidant Activity Of The Column C...mentioning

confidence: 99%

Column Chromatography Fractionation and Antioxidant Activity of Passiflora foetida Leaves

Triadisti¹,

Zamzani²

2023

Borneo J Pharm

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Available synthetic antioxidants have been reported to have mutagenic and toxic effects. On the other hand, natural antioxidants show their superiority as they are not or less toxic. Passiflora foetida has the potential as an antioxidant, but the investigation of the antioxidant activity of the P. foetida chromatography column fraction has not been reported. This studied aims to investigate the antioxidant activity of the column chromatographic fractions of P. foetida leaves. An antioxidant assay using the DPPH and FRAP methods. The extraction was carried out by graded maceration, then fractionation using column chromatography. The antioxidant activity test was carried out using the DPPH and FRAP methods. Thin Layer Chromatography analysis was performed to determine the chromatogram pattern. The EC50 using DPPH method from n-hexane extract: 129.035 µg/mL, ethyl acetate extract: 206.398 µg/mL, methanol extract: 97.453 µg/mL, while the EC50 using FRAP method from n-hexane extract: 67.851 µg/mL, ethyl acetate extract : 68.981 µg/mL, and methanol extract: 58.787 µg/mL. Column chromatography fractions have antioxidant activity, with FMetPF6 as the fraction with the best activity, with percent inhibition 41.85±1.96 at concentration 25 µg/mL (DPPH), and with percent antioxidant activity 26.03±0.84 at concentration 9 µg/mL (FRAP). Passiflora foetida leaves have great potential as an antioxidant; both the extract and its fractions have antioxidant activity. The FMetPF6 has the best activity compare to other extracts and fractions. Further analysis to determine the various compounds in FMetPF6 using LC-MS/MS will facilitate the active compound's isolation.

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AKTIVITAS ANTIOKSIDAN FRAKSI DARI DAUN Garcinia kydia Roxburgh

Martha¹,

Elya

Hanafi

2020

MFF

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Garcinia kydia Roxburgh merupakan salah satu spesies dari genus garcinia, yang secara khusus belum banyak diketahui aktivitas senyawa bioaktif, namun pada penelitian sebelumnya terbukti berpotensi sebagai agen penghambat α-glukosidase. Pada penelitian ini, akan dilakukan investigasi aktivitas antioksidan terhadap berbagai fraksi yang diperoleh dari daun G. kydia Roxb. Metode penelitian dilakukan dengan menentukan aktivitas antioksidan melalui analisis in-vitro menggunakan microplete reader dengan metode radical scavenging (Metode DPPH) dan ferric reducing antioxidant power (Metode FRAP). Hasil menunjukkan bahwa fraksi yang paling aktif dari ekstrak etil asetat adalah FEA8 (DPPH) dan FEA7 (FRAP), sedangkan fraksi paling aktif dari ekstrak metanol adalah FMT4 (DPPH) dan FMT6 (FRAP). Aktivitas antioksidan tertinggi di antara empat fraksi adalah FEA7 dan FEA8. Aktivitas fraksi FEA7 dan FEA8 ditentukan oleh nilai EC50, sehingga diperoleh masing-masing 9,73 dan 9,26 μg/mL (DPPH); 10,28 dan 13,94 μg/mL (FRAP). Garcinia kydia Roxb. berpotensi sebagai agen antioksidan dengan mekanisme yang berbeda. Sehingga hasil penelitian terkait terdapatnya potensi antioksidan tersebut dapat memberikan alternatif terapi diabetes yang disertai komplikasi stres oksidatif.

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COMPARISON OF INHIBITORY ACTIVITY AGAINST THE Α-Glucosidase ENZYMES IN THE EXTRACTS AND FRACTIONS FROM LEAVES OF THE GARCINIA KYDIA ROXBURGH

Cited by 3 publications

References 15 publications

The Antioxidant and Hypoglycemic Properties and Phytochemical Profile of Clusia latipes Extracts

The Antioxidant and Hypoglycemic Properties and Phytochemical Profile of Clusia latipes Extracts

Column Chromatography Fractionation and Antioxidant Activity of Passiflora foetida Leaves

AKTIVITAS ANTIOKSIDAN FRAKSI DARI DAUN Garcinia kydia Roxburgh

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