The objective was to evaluate the use of subcuticular absorbable staples (SAS) and compare them with metallic staples (MS) in abdominal skin closure for equine abdominal surgery in terms of surgical site infection (SSI) reduction. A prospective, randomised clinical trial was carried out on client owned horses presenting for abdominal surgery (n = 42 horses). During surgery, horses were randomly assigned to receive either SAS or MS. Preoperative criteria recorded included age, breed and sex of horse; surgical variables included lesion type, anaesthetic, surgical and stapling times; post operative variables included an anaesthetic recovery quality score and an incisional assessment score. Mean ± s.d. anaesthesia times for small intestinal surgeries between the MS and SAS (228 ± 58.8 min, 234 ± 38.7 min, respectively) and surgical times between MS and SAS (158 ± 58.8 min, 174 ± 26.5 min, respectively) were not significantly different from each other. There was no significant difference in closure times for the MS and SAS (61 ± 14.