“…Single-section techniques have been used to investigate demineralization and remineralization of enamel and dentine in vitro [Featherstone and Silverstone, 1982;Harvey et al, 1982;ten Cate and Exterkate, 1986;Strang et al, 1988;Lammers et al, 1990;Exterkate et al, 1993;Inaba et al, 1995] as well as in situ [Creanor et al, 1986;Mellberg et al, 1986Mellberg et al, , 1992Mellberg, 1992;Wefel et al, 1987;Wefel, 1990;Strang et al, 1987;Stephen et al, 1992;Damato and Stephen, 1994]. Two types of single-section techniques are currently used to create lesions in enamel or dentine.…”