Comparisons of in vitro Root Caries Models

Abstract

The purpose of this article is to compare various model systems for the production of in vitro root caries and to assess their ability to simulate the naturally occurring root caries process. Partially saturated buffer models and gel models were evaluated using polarized light microscopy and both qualitative and quantitative microradiography. All model systems showed very similar lesion formation when examined under polarized light. When microradiographs were compared, the systems which contained fluoride, showed clear radiopaque bands within the lesion. The bands, which occurred only in the presence of fluoride, appeared to be due to remineralization. When using an in vitro system that simulates the natural root caries process, it is imperative to understand the components of the particular model, as well as its limitations, and to be aware of the need for more than one evaluative technique.

Effect of hybridization on bond strength and adhesive interface after acid-base challenge using 4-META/MMA-TBB resin

“…The demineralizing solution has been known to create artificial secondary caries around dental restorations 16) . The pH value of this solution was adjusted to 4.5, which was much milder as compared to the 10-3 solution and phosphoric acid.…”

“…Subsequently, the specimens were vertically sectioned at the dentin-adhesive interface and embedded in an epoxy resin (Epoxicure Hardener, Buehler Ltd., Lake Bluff, IL, USA). For the acid challenge, the specimens were immersed in a buffered demineralizing solution (2.2 mmol/L CaCl2, 2.2 mmol/L NaH2PO4, and 50 mmol/L acetic acid) adjusted to pH 4.5 16) for 90 minutes. After the acid challenge, the specimens were immersed in 5％ sodium hypochlorite solution for 20 minutes to remove any demineralized dentin collagen fibers, and then rinsed with running water for 60 seconds.…”

Evaluation of dentin bonding performance and acid-base resistance of the interface of two-step self-etching adhesive systems

“…The bonded specimens were stored in distilled water at 37°C for 24 h. Subsequently, the specimen was vertically sectioned at the dentin-adhesive interface and then embedded in an epoxy resin (Epoxicure Resin, Buehler). Following this, the specimens were first stored in 100 ml of a buffered demineralizing solution, containing 2.2 mmol/ L CaCl2, 2.2 mmol/L NaH2PO4 and 50 mmol/L acetic acid adjusted at pH 4.5 for 90 min to create artificial secondary caries 18) . The specimens were then immersed in 5% NaClO for 20 min in an attempt to remove any demineralized dentin collagen fibrils, and rinsed with running water for 30 s. Following this, 4-META/MMA-TBB resin (Super Bond C&B, Sun Medical, Moriyama, Japan) was applied without acid etching to the treated surface in order to protect the demineralized surface from the polishing procedure.…”

Influence of FCP-COMPLEX on bond strength and the adhesive–artificial caries-affected dentin interface

“…Thereafter, sectioned specimens were embedded in epoxy resin (Epoxicure2, Buehler). The specimens were subjected to the acid-base challenge using a demineralizing solution (pH 4.5, 2.2 mM CaCl 2, 2.2 mM Na2HPO4, 50 mM acetic acid) for 90 min, and 6% NaOCl for 20 min 11) . After the acid-base challenge, a self-curing resin (Super Bond C&B, Sun Medical, Moriyama, Japan) was put on the demineralized surface There were no significant differences among all groups (p>0.05, two-way ANOVA with post hoc test.).…”