Comparison of immunofluorescence assay (IF) with ELISA in detection of antinuclear antibodies

Osteoporosis is a bone disorder characterized by the decrease of bone mass along with bone micro-architecture damage and has a risk become a fracture. One of the causes of osteoporosis is estrogen deficiency. Genistein is a phytoestrogen compound in the isoflavone group containing a similar structure compared to 17β-estradiol, thus it can bind to estrogen receptors and produce an estrogenic effect. Genistein induction can stimulate bone formation and promote the increase of alkaline phosphate (ALP) activities in osteoblast cells which can be observed by immunocytochemistry or Enzyme-linked Immunosorbent Assay (ELISA) or Western blot method. Using the PRISMA guideline technique, choose and strategize article searches by reading the title, abstract, and then the whole text of the article. Articles with the keywords "genistein or osteoblast cells or alkaline phosphate or immunocytochemistry or immunofluorescence or ELISA or western blot" were retrieved from databases including Google Scholar, PubMed, Researchgate, and Sciencedirect. 24 relevant research articles were uncovered as a result of this systematic review. Comparison of immunocytochemistry and ELISA methods in order to analyze the activities of ALP in osteoblast induced by genistein includes selectivity, sensitivity, processing time, and cost efficiency parameters. The immunocytochemistry method has a higher level of sensitivity and a faster processing time, whereas the ELISA method has a higher level of selectivity and less cost efficiency. The western blot method has selectivity for detecting complex-level protein expression.

show abstract

A Systematic Review: Comparison of Immunocytochemistry, ELISA, and Western Blot Methods in Alkaline phosphatase Measurement at Genistein-induced Osteoblast Cell

Ma’arif

Abada

Mahardiani

et al. 2022

Biomed. Pharmacol. J.

View full text Add to dashboard Cite

show abstract

Prevalence of Anti-Nuclear Antibody in the Indian Population: A Tertiary Reference Laboratory Study

Chadha¹,

Puranik

Sundharan³

et al. 2022

IJAR

View full text Add to dashboard Cite

Background: Autoimmune diseases are known to be the third leading cause of fatality and morbidity amongst the population of industrialized world. They account for 3-9% of health burden in general people, but information regarding prevalence of autoantibodies and autoimmune diseases in developing nations is scarce. To study the prevalence of Anti-nuclear antibodies, Aim: the total number and distribution of different tests used in the diagnosis of anti-nuclear antibody amongst Indian population, and correlate the ndings from these tests with the clinical characteristics of the patients. Retrospective data was evaluated from a Global Reference Method: Diagnostic Laboratory in Mumbai, for a period of 6 years. This included a total of 285095 cases tested for ANA. ANA-IFA and ANA-ELISA were the screening tests used while ANA-ELISA Prole and ANA Blot were the conrmatory tests. ANA by IFA was the most preferred Results: screening test (88.73%) and ANA by Blot was the most preferred Conrmatory test (67.13%) based on their sensitivity and positive predictive value respectively. ANA-IFA showed positivity of 36.48% and ANA by ELISA test had positivity of 11.46%. In conrmatory testing, ANA Blot showed a positivity of 31.90% and ELISA Prole had 23.36% positivity. Females showed signicantly higher positivity for both the screening test and Conrmatory tests than males (p<0.001). Screening by ANA IFA and Conrmatory by Conclusion: ANA Blot was the most preferred tests in our study population. These tests were found to be better for diagnosis, sub-syndrome categorization, prognosis, clinical follow-up and therapeutic strategies in various autoimmune disorders.

show abstract

“Correlation of Specific Ana(iif) Pattern and Antibodies Detected in Ana Blot Test in Various Autoimmune Disorders: A Prospective Study”

Bokey

Deshmukh

Soni

et al. 2022

ijsr

View full text Add to dashboard Cite

Background: One of the central features of the immune system is the capacity to mount an inammatory response to potentially harmful foreign materials while avoiding damage to self-tissues. Autoimmunity is dened as an immune response leading to reaction with self-antigen, this may be a harmless phenomenon, identied only by the presence of low-titre autoantibodies or autoreactive T cells. These are a major cause of chronic morbidity and disability, affecting up to 1 in 30 adults at some point during life. Autoimmune diseases result from the failure of immune tolerance, the process by which the immune system recognises and accepts self-tissue. Materials And Methods Hospital based Observational study. Total 152 cases were studied with suspected connective tissue disorders attending OPD/IPD in tertiary care hospital in One and a half years (January 2020 to July 2021), but only 57 out 0f 152 cases were screened by ANA IIF Test and ANA BLOT Test both. Result: ANA positivity was more observed in females. Autoimmune disorder was more common in the age group of 20-40 years about 22 cases (38.6%). Most common pattern observed was Homogenous (n=20, 48.78%). Most common band observed is dsDNA (14.92%) followed by SS/RO 60 (13.43%) PO (11.94%), Histone (10.44%) and Nucleosome (10.44%). The sensitivity, specicity, positive predictive value, negative predictive value of ANA Blot after comparing it with ANA IIF is 70.73 %, 87.50%, 93.54%, 53.84% respectively. Conclusion: In conclusion, detection of ANA by indirect immunouorescence is the most cost - effective screening test in investigating autoimmune disease. ALine-Immuno Assay is performed for further conrmation and detection of specic antibody in sera which can be quantitated with ELISA/RIA/CLIA methods and can help in assessing the prognosis, disease activity and monitoring of therapy for a particular auto-immune disorder. However, despite their easy use, line immunoassays have some drawbacks including lack of sensitivity and specicity for certain antigens.

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

hi@scite.ai

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Comparison of immunofluorescence assay (IF) with ELISA in detection of antinuclear antibodies

Cited by 3 publications

References 8 publications

A Systematic Review: Comparison of Immunocytochemistry, ELISA, and Western Blot Methods in Alkaline phosphatase Measurement at Genistein-induced Osteoblast Cell

A Systematic Review: Comparison of Immunocytochemistry, ELISA, and Western Blot Methods in Alkaline phosphatase Measurement at Genistein-induced Osteoblast Cell

Prevalence of Anti-Nuclear Antibody in the Indian Population: A Tertiary Reference Laboratory Study

“Correlation of Specific Ana(iif) Pattern and Antibodies Detected in Ana Blot Test in Various Autoimmune Disorders: A Prospective Study”

Contact Info

Product

Resources

About