Comparison of immediate intradermal test reactivity with serum IgE quantitation by use of a radioallergosorbent test and two ELISA in horses with and without atopy

Lorch, Gwendolen; Hillier, Andrew; Kwochka, Kenneth W.; Saville, William J.; Kohn, Catherine W.; LeRoy, Bruce E.

doi:10.2460/javma.2001.218.1314

Cited by 55 publications

(56 citation statements)

References 33 publications

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“…Allergic disease (hypersensitivity to insect and environmental allergens) has been implicated in the pathophysiology of AD, RU, and RAO [1] and may be associated with IgE-mediated hypersensitivity. They often result in pruritus and self excoriation and on rare occasions may affect athletic performance and even prompt euthanasia [8] .…”

Section: Discussionmentioning

confidence: 99%

“…In horses, allergic disease (hypersensitivity to insect and environmental allergens) has been implicated in the pathophysiology of atopic dermatitis (AD), recurrent urticaria (RU), and recurrent airway obstruction (RAO) [1] . Diseases in horses such as AD, RU, and RAO [2] have long been thought to have an allergic etiology due to similarities with human allergies which have been shown to be mediated by IgE [3] .…”

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confidence: 99%

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Allergen Determination in Thoroughbred Stallions via Detecting Serum Specific IgE

Pekmezci¹,

Sancak²,

Pınar³

et al. 2013

Kafkas Univ Vet Fak Derg

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SummaryIn horses, allergic disease has been implicated in the pathophysiology of atopic dermatitis, recurrent urticaria, and recurrent airway obstruction. With the present study, it is aimed to give information about allergens which determined in Thoroughbred stallions with detecting serum IgE. 18 Thoroughbred stallions averaged 11 years old were included in the study. Fifteen single and five mix allergens were checked for allergen-specific IgE. English plantain (Plantago lanceolata), Birch/Alder/Hazel and Micropolyspora faeni/Thermoactinomyces were detected as quite allergic to six Thoroughbred stallions at total. Consequently, assays detect equine IgE, can be an effortless method in determining allergens in horses. Keywords: Allergen, IgE, Stallion, Thoroughbred, Urticaria İngiliz Aygırlarında Serum Spesifik IgE Tespiti ile Alerjen Tayini ÖzetAtlarda alerjik hastalıklar atopik dermatitis, tekrarlayan ürtiker ve tekrarlayan havayolu hastalıklarının patofizyolojisinde rol oynamaktadır. Bu çalışma ile İngiliz aygırlarında serum spesifik IgE tespiti ile alerjenlerin tayini hakkında bilgi verilmesi amaçlanmıştır. Yaşları ortalama 11 olan, 18 İngiliz aygır çalışma materyalini oluşturmuştur. On beş tek ve beş karışık alerjene serum spesifik IgE yönünden bakılmıştır. Toplam altı İngiliz aygırda English plantain (Plantago lanceolata), Birch/Alder/Hazel ve Micropolyspora faeni/Thermoactinomyces oldukça alerjik olarak tespit edilmiştir. Sonuç olarak, atlara özgü IgE'yi belirleyen serolojik testler alerjenlerin tespiti bakımından zahmetsiz bir yöntem olarak değerlendirilebilir.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Allergen Determination in Thoroughbred Stallions via Detecting Serum Specific IgE

Pekmezci¹,

Sancak²,

Pınar³

et al. 2013

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

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“…Κ can be used to assess multiple levels of agreement: less than 0 ¼ disagreement, 0-0.2 ¼ slight agreement, 0.2-0.4 ¼ fair agreement, 0.4-0.6 ¼ moderate agreement, 0.6-0.8 ¼ substantial agreement, and 0.8-1 ¼ almost perfect agreement [36].…”

Section: Statistical Analysesmentioning

confidence: 99%

“…The test was performed by the CMG Heska Laboratory, as previously described by Lorch et al [36], and the laboratory was not aware of the SPT results.…”

Section: Determination Of Allergen-specific Ige Levels In Serum and Balfmentioning

confidence: 99%

Comparison of Skin Prick Tests with In Vitro Allergy Tests in the Characterization of Horses with Recurrent Airway Obstruction

Tilley¹,

Luis²,

Ferreira³

2012

Journal of Equine Veterinary Science

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“…However, these investigations have been hampered by problems with the use of antibodies for detection of equine IgE (Tahon et al 2009). Actually, few studies have been performed with monoclonal antibodies (Wagner et al 2003) or with the α chain of the IgE receptor (Lorch et al 2001a) to allow specific detection of equine IgE. Additionally, direct testing of the horse's skin has not proven useful for diagnostic purposes since most horses give a positive skin reaction to common barn allergens, even though horses with RAO tend to have a stronger positive skin reaction (Eyre 1972;Schatzmann and Gerber 1972;Evans et al 1992;Mcgorum et al 1993;Lorch et al 2001b).…”

Section: Introductionmentioning

confidence: 98%

In vitro bioassay to detect reaginic antibodies from the serum of horses affected with Recurrent Airway Obstruction

et al. 2009

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In horses, Recurrent Airway Obstruction (RAO) is an allergic disease that involves IgE mediated Type I Hypersensitivity responses. The development of this type of allergy involves a series of events that begins with reaginic antibodies, mainly IgE and some IgG subclasses. These reaginic antibodies bind with high affinity, via the Fc portion, to FcepsilonRI receptors on the membrane of mast cells and basophils. Once bound, environmental allergens cross-link the antibodies, which results in mast cell degranulation leading to the production of histamine and other chemical mediators that act together to induce airway inflammation. RAO-affected horses present with coughing, respiratory distress, airway obstruction and poor performance. The aspect of the RAO has been extensively studied, yet the precise sequence of events is still not well understood. Therefore, this study proposes a bioassay for reaginic antibody detection from horse serum of RAO-affected individuals, in order to determine the etiology of disease, which mediate immediate type reactions. The technique involves measuring in vitro calcium mobilization in RBL-2H3 cells following incubation with horse serum from affected or unaffected horses and one of the RAO antigens (Aspergillus fumigatus). The results presented here demonstrate that 30% of RAO-affected horses react positively in this in vitro bioassay, whereas unaffected horses do not. This bioassay may facilitate further research on RAO and other allergic diseases in horses.

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Comparison of immediate intradermal test reactivity with serum IgE quantitation by use of a radioallergosorbent test and two ELISA in horses with and without atopy

Cited by 55 publications

References 33 publications

Allergen Determination in Thoroughbred Stallions via Detecting Serum Specific IgE

Allergen Determination in Thoroughbred Stallions via Detecting Serum Specific IgE

Comparison of Skin Prick Tests with In Vitro Allergy Tests in the Characterization of Horses with Recurrent Airway Obstruction

In vitro bioassay to detect reaginic antibodies from the serum of horses affected with Recurrent Airway Obstruction

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