Comparison of haematological parameters determined by the Sysmex KX - 2IN automated haematology analyzer and the manual counts

Abstract

BackgroundThis study was designed to determine the correlation between heamatological parameters by Sysmex KX-21N automated hematology analyzer with the manual methods.MethodSixty (60) subjects were randomly selected from both apparently healthy subjects and those who have different blood disorders from the University of Teaching Hospital (UNTH), Ituku-Ozalla, Enugu, Enugu State, Nigeria. Three (3)mls of venous blood sample was collected aseptically from each subject into tri-potassium ethylenediamine tetra-acetic acid (K3EDTA) for the analysis of haematological parameters using the automated and the manual methods.ResultsThe blood film report by the manual method showed that 50% of the subjects were normocytic-normochromic while the other 50% revealed different abnormal blood pictures. Also, there were statistically significant differences (p < 0.05) in mean cell hemoglobin concentrations (MCHC) between the two methods. Similarly, the mean (S.E) values of hemoglobin, packed cell volume, platelet and total white cell counts demonstrated statistically significant difference (p < 0.001) and correlated positively when both methods were compared.ConclusionFrom the present study, it can be concluded that the automated hematology analyzer readings correlated well with readings by the standard manual method, although the latter method gave additional diagnostic information on the blood pictures. While patients' care and laboratory operations could be optimized by using manual microscopic examination as a reflective substitute for automated methods, usage of automated method would ease our workload and save time for patients.

The Administration of Garlic Extract on Eimeria stiedai Oocysts and the Hematological Profile of the Coccidia Infected Rabbits

“…Phytochemical and proximate analyses of garlic powder were also respectively conducted at Indonesian Spice and Medicinal Crops Research Institute and Animal Feed Material Laboratory, Faculty of Animal Sciences, Jenderal Soedirman University. The hematological analyses covering the erythrocytes, hemoglobin, hematocrits, MCV, MCH, MCHC, and thrombocytes were conducted at Biofit in Purwokerto by The Sysmex XP-100 automated hematology analyzer (Ike et al, 2010;Karem et al, 2016).…”

Determination of haematocrit using Mindray BC-2800Vet^® automated haematology analyser and microhaematocrit method: A comparative study

“…The haematocrit values obtained through the manual and automated procedures correlate well (Ike et al, 2010;Kaznowska-Bystryk, 2011;Gebretsadkan et al, 2015). However, despite the strong positive correlation between the two methods, the spun haematocrit is sometimes significantly higher or lower than automated haematocrit (Bull & Hay, 2001;Ike et al, 2010;Gebretsadkan et al, 2015). This is the first report of comparison of manual and automated haematocrit analyser designed for Veterinary use in Nigeria to the best of our knowledge.…”

“…The automated haematology analyser has better precision than the manual microhaematocrit (Gebretsadkan et al, 2015). The haematocrit values obtained through the manual and automated procedures correlate well (Ike et al, 2010;Kaznowska-Bystryk, 2011;Gebretsadkan et al, 2015). However, despite the strong positive correlation between the two methods, the spun haematocrit is sometimes significantly higher or lower than automated haematocrit (Bull & Hay, 2001;Ike et al, 2010;Gebretsadkan et al, 2015).…”

“…This is the first report of comparison of manual and automated haematocrit analyser designed for Veterinary use in Nigeria to the best of our knowledge. Previous comparisons were carried out with machines designed for use in human specimens (Ike et al, 2010), where automated haematology analyser gave a better precision as well as save time and labour cost, compared to the manual method. The cost of the auto analyser and consumables makes it unavailable for field use.…”

The Effects of Aqueous Extracts of &lt;i&gt;Acalypha wilkesiana&lt;/i&gt; Supplementation and Exercise Training on Hematopoietic System in Rats

“…It employs WBC detector block and WBC/HGB lyse reagent to measure WBC count and Hemoglobin concentration as described [20].…”