This research aimed to examine the potential of garlic as the coccidiosis control in rabbits either in vitro or in vivo. During in vitro, observed variables were rabbits oocysts that were sporulated, unsporulated, and abnormal in incubation for 3 days with the addition of garlic extract. The treatments were doses of garlic extract administration (0%, 1%, 2%, 4%, and 8%) and sulfaquinoxalline as a standard anticoccidiosis. Meanwhile during in vivo, the variables observed were the hematological profile of the experimental rabbits naturally infected with coccidia. The doses of garlic extract was administered orally to the experimental rabbits infected with coccidia were 0 mg, 10 mg, 20 mg, 40 mg, and 80 mg/ rabbit. As a standard coccidiosis drugs, the combination of sulfadiazine and trimethoprim was used. The treatments were given for 6 days. The variables observed were the hematological profile of the coccidiosis rabbits, including the erythrocytes, hemoglobin, hematocrits, MCV, MCH, MCHC, and thrombocytes. The research employed a completely randomized design, with 5 repetitions. The data were further analyzed using the honestly significant difference test. The results showed that garlic extract administration significantly decreased (P<0.01) both the number of the sporulated and unsporulated oocysts (P<0.05), yet did not significantly influence the abnormal oocysts, but there was no significant difference within the entire hematological variables except in thrombocytes (P<0.05). Garlic extract administration decreased the excretion number of oocysts in the feces either in vitro or in vivo and influenced some hematological variables which provided a new propect for controlling coccidiosis naturally in rabbits.Keywords: coccidia, rabbits, garlic extract, oocyst, hematology ABSTRAK Penelitian ini bertujuan untuk menguji potensi bawang putih sebagai anti koksidiosis pada kelinci secara in vitro dan in vivo. Secara in vitro, peubah yang diamati adalah ookista kelinci yang bersporulasi, tidak bersporulasi, dan abnormal pada inkubasi selama 3 hari dengan penambahan ekstrak bawang putih. Perlakuan yang digunakan adalah 0%, 1%, 2%, 4%, dan 8% bawang putih, serta penggunaan sulfaquinoxalline sebagai obat standar koksidiosis. Pada penelitian in vivo, peubah yang diamati adalah profil hematologi kelinci yang terinfeksi koksidia secara alami. Ekstrak bawang putih diberikan secara per oral pada kelinci yang terinfeksi koksidia sebesar 0 mg, 10 mg, 20 mg, 40 mg, dan 80 mg/ekor, serta kombinasi sulfadiazin dan trimethoprim sebagai obat standar koksidiosis. Perlakuan diberikan selama 6 hari. Peubah yang diamati adalah profil darah kelinci koksidiosis yang meliputi sel darah merah (eritrosit), hemoglobin (Hb), hematokrit, MCV, MCH, MCHC, dan trombosit. Rancangan yang digunakan adalah rancangan acak lengkap (RAL), dengan pengulangan sebanyak 5 kali. Analisis data menggunakan analisis variansi dengan uji lanjut beda nyata jujur (BNJ). Hasil analisis varians menunjukkan bahwa pemberian ekstrak bawang putih berpengaruh sangat ...

Abstract. The objective of this research was to investigate the ability of banana stem (Musa paradisiaca) to inhibit sporulation of Eimeria stiedai oocysts derived from rabbit by in vitro analysis. Analyze the active substance proximate analysis and active substances in this research were performed too. Banana stem extract were used in this experiment and sulfaquinoxalline (Coxy®) was run as a control. The Eimeria stiedai oocysts were incubated prior the presence of different concentration from banana stem extract 0%, 1%, 2%, 4%, 8% for 1, 2 and 3 days at 26°C. In addition, Factorial patterned Completely Randomized Design (CRD) with five replicates was applied on the experiment. Result analysis was performed by using Analysis of Variance and following by Honestly Significant Difference (HSD) post hoc test. Here, we identified that banana stem extract contain different type of active substance such as tannin, saponin, and alkaloid. Banana stem extract significantly affected the oocysts sporulation included the amount of sporulated oocysts (P<0.01), unsporulated oocysts (P<0.01), and transformed oocysts (P<0.01). In conclusion banana stem could inhibit the development of Eimeria stiedai oocysts on in vitro experiment. HSD test showed that the optimum potential efficacy of banana stem to inhibit sporulation was at 4% and 8% concentration during three days incubation. Key words: Eimeria stiedai, rabbit coccidiosis, banana stemAbstrak. Penelitian ini bertujuan untuk mengetahui kemampuan batang pisang (Musa paradisiaca) untuk menghambat kemampuan sporulasi ookista Eimeria stiedai kelinci melalui analisis secara in vitro. Pada penelitian ini juga dilakukan analisis proximat dan kandungan fitokimia ekstrak batang pisang. Ekstrak batang pisang digunakan pada penelitian ini dan kontrol sulfaquinoxalline (Coxy®). Ookista Eimeria stiedai kelinci diinkubasi dengan konsentrasi yang berbeda yaitu 0%, 1%, 2%, 4%, 8% selama 1,2 dan 3 hari pada suhu 26 °C bersama ookista Eimeria stiedai selama 1,2 dan 3 hari. Rancangan penelitian yang digunakan adalah Rancangan Acak Lengkap (RAL) pola faktorial, dengan pengulangan sebanyak 5 kali. Hasil analisis ditentukan dengan analisis variansi dilanjutkan uji lanjut Beda Nyata Jujur (BNJ). Ekstrak batang pisang mengandung beberapa zat aktif yaitu tanin, saponin dan alkaloid. Ekstrak batang pisang berpengaruh signifikan terhadap kemampuan sporulasi ookista yang meliputi jumlah ookista bersporulasi (P<0,01), ookista yang tidak bersporulasi (P<0,01) dan ookista yang mengalami perubahan bentuk (P<0,01). Kesimpulan dalam penelitian ini bahwa batang pisang mampu menghambat perkembangan koksidia Eimeria stiedai secara in vitro. Uji BNJ menunjukkan potensi efektifitas terbaik ekstrak batang pisang diperoleh secara in vitro terhadap E. stiedai pada konsentrasi 4% dan 8% dengan lama inkubasi 3 hari.

The worm infections can cause a decrease in livestock productivity, were in the form of a decrease in body weight, milk production, growth restriction, decrease immunity to disease, even death. Research on worm disease in Boer goats aims to determine the infection rate of Nematodiasis and Trematodiasis in goats with various age levels, young and adult age groups. This research method is survey with sampling technique is sampling by the judgment. The data analysis of the research was prevalence study and Chi-Square analysis (X2). The examination of worm eggs in feces on the research used centrifuge method. The results of examination on 30 samples of Boer goats showed that 93.33% of Boer goats were infected by Nematodes and 13.33% of Boer goats were infected by Trematoda. Infection rates of Nematodes and Trematoda were not showed any significant differences between the two groups. The low level of Trematodiasis in Boer goats indicates that Boer goats in Banyumas district are resistant to Trematoda worm infections. Keywords: Boer Goats, Centrifuge Method, Chi-Square Analysis, Infection Rate, Worm Infection

Increased productivity of rabbits was through improved feed. Katuk plant has high nutritional value and its leaves contain nutrients needed by the body. The protein content in feed greatly affects the character of the blood. The purpose of this study was to optimize the effect of using katuk leaf supplementation on the number of erythrocytes and the hemoglobin value of rex rabbits. The research material were 18 rex rabbits, basal feed added with katuk leaves. The research method used was Completely Randomized Design (CRD) with three treatments, each treatment repeated six times, so that there were 18 experimental units. The treatments were R0: basal diet without katuk leaf supplementation, R1: basal diet with 5% katuk leaf supplementation, R2: basal diet with 10% katuk leaf supplementation. The variables measured were the number of erythrocytes and the value of hemoglobin. Data were analyzed using analysis of covariance (ANCOVA). The results showed that rabbit rex erythrocytes were R0 = 4.7 ± 0.39 x 106/µL; R1 = 4.99 ± 0.28 x 106/µL; R2 = 4.76 ± 0.31 x 106/µ. The average result of the three treatments above was 4.83 ± 0.83 x 106/µL. The erythrocyte mean results were still in the normal range. The results of the analysis of variance showed that the three treatments did not show significant differences. The conclusion of the study was that katuk leaf supplementation can be given to rabbit rex up to a level of 10% without affecting the physiological process of livestock in terms of the number of erythrocytes and hemoglobin values which are relatively the same.Key words : erythrocytes, hemoglobin, katuk leaves, rabbit rex.

This study aims to determine oocysts excretion and hematological profile in coccidiosis rabbits given a combination of herbal extract II. Hematological profiles observed were red blood cells (RBC), white blood cells (WBC), hemoglobin (HGB), hematocrit (HCT), granulocytes, eosinophils, monocytes, lymphocytes, Mean Corpuscular Volume (MCV), Mean Corpuscular Hemoglobin (MCH) and Mean Corpuscular Hemoglobin Concentration (MCHC). This study used 40 rabbit coccidiosis material with ± 3 months age of ± 650 g weight, a combination of herbal extracts consisting of banana stem extract (BSE), papaya seeds (PSE) and garlic (GE), a set of tools and materials for rabbit maintenance and a set of hematological examination tools. The research method was carried out experimentally using a Completely Randomized Design (CRD). The analysis used variance analysis followed by Honest Real Difference (HRD). The combination of herbal extract II consists of BSE: 40 mg; PSE: 20 mg; GE: 40 mg. Rabbits were divided into 8 treatments with 5 replications, namely giving a combination of herbal extracts 0 mg (D0), 10 mg (D1), 20 mg (D2), 40 mg (D3), 80 (D4) mg, 100 mg (D5) and the comparison are used herbal extract I (consist of BSE: 33 mg; PSE: 2 mg; GE: 65 mg) as much as 100 mg (D6) and Aquaprime® (D7). Blood collection is carried out through the heart on the 14th day after treatment. The combination of herbal extract II had a very significant effect on oocysts excretion, but did not have a significant effect on all hematology parameters. Hence, a combination of herbal extracts can be used as an alternative to reduce the number of oocysts in rabbits coccidiosis.

The objective of this research was to investigatethe ability of banana stem (Musa paradisiaca) to inhibitsporulation of Eimeria stiedaioocystsderived fromrabbit by in vitroanalysis.Analyze the active substance proximate analysis and active substancesin this research were performed too. Banana stem extract were used in this experiment andsulfaquinoxalline(Coxy ®)was run as acontrol. The Eimeria stiedaioocystswere incubated prior the presence of  different concentration from banana stem extract  0%, 1%, 2%, 4%, 8%for 1, 2 and 3 daysat 26°C. In addition,Factorial patterned Completely Randomized Design (CRD) with five replicates wasapplied on the experiment. Result analysis was performed by using Analysis of Variance and following by Honestly Significant Difference (HSD) post hoc test. Here, we identified that banana stem extract contain different type of active substance such as tannin, saponin, and alkaloid. Banana stem extract significantly affected the oocysts sporulation included the amount of sporulatedoocysts (P<0.01), unsporulatedoocysts (P<0.01), and transformed oocysts (P<0.01). In conclusion banana stem could inhibit the development of Eimeria stiedaioocysts on in vitroexperiment. HSD test showed that the optimum potential efficacy of banana stem toinhibit sporulation was at 4% and 8% concentration during three days incubation.

The objective of the research was to investigate body weight, oocyte elimination and blood profile of rabbits infected with various doses of Eimeria stiedai isolates. The observed rabbits’ blood profile included erythrocyte, hemoglobin, hematocrit, leucocyte, thrombocyte, total protein plasma (TPP) and fibrinogen. Twenty-five male New Zealand White rabbits aged 3 months and weighed approximately 2 kg were provided with pellet and boiled drinking water and Eimeria stiedai isolates. The experiment used Completely Randomized Design to analyze 5 treatments with five replicates. The examined variables included D0: Infection 0 (control of infection without challenge test), D1: Infection 101 with challenge test 103, D2: infection 102 with challenge test 103, D3: infection 103 with challenge test 103, D4: infection 0 with challenge test 103 (control of infection). Data were subject to analysis of variance followed by Honestly Significant Difference Test (HSD). Analysis of Variance result showed that there was no significant difference on body weight, oocyte elimination and blood profile including erythrocyte, hemoglobin, hematocrit, leucocyte, thrombocyte, and fibrinogen. However, total protein plasma (TTP) was significantly different at 5% HSD. It can be concluded that challenge test with Eimeria stiedai has not been used as an alternative in increasing rabbits’ body immune against coccidiosis infection.

The purpose of this study was to make a linear model of the leukogram variable with the number of oocysts in cases of coccidiosis infection in rabbits. The infection response in illness of disease course and will be linearly proportional to the rate of severity that occurs. Blood samples obtained from 91 rabbits were examined to determine the value of their lekogram component, as well as 91 rabbit feces were examined to determine the number of livestock can be described in the dynamics of the components of white blood cells (leukocytes), the increased response to infection clinically and haematologically will run parallel to the coccidia oocysts. The quantitative data from the count of oocysts with lekogram values were analyzed using a multivariate regression model, to determine the best linear model that could describe the severity of coccidiosis infection with the lekogram value, with the number of oocysts as the dependent variable and the lecogram values as the independent variable, the best model of linearity can be used to predict disease severity. Of all the lekogram variables analyzed, the amount of total fibrinogen had a significant effect (P <0.05) on the severity of Coccidiosis, with the linear formula Y = 2.7 + Fibrinogen Value, so that the fibrinogen value could be used to predict the severity of Coccidiosis in rabbits.