Comparison of Gold Nanoparticle Conjugated Secondary Antibody with Non-Gold Secondary Antibody in an ELISA Kit Model

Emami, Tara; Madani, Rasool; Golchinfar, Fariba; Shoushtary, Abdolhamid; Amini, Seyed Mohammad

doi:10.1089/mab.2015.0021

Cited by 22 publications

(11 citation statements)

References 9 publications

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“…Interesting properties, like surface plasmon resonance (SPR) and instinct biocompatibility, make gold nanostructures as useful tools in many types of detection/diagnosis and treatment techniques [5][6][7]. One of these interesting methods is hyperthermia that uses gold nanostructures in two different approaches.…”

Section: Different Types Of Gold Nanostructures Have Found Unique Capmentioning

confidence: 99%

Radiofrequency electric field hyperthermia with gold nanostructures: role of particle shape and surface chemistry

Amini

Rezayat

Gilani

2017

Artificial Cells, Nanomedicine, and Biotechnology

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Hyperthermia treatment of cancerous cells has been recently developed drastically with the help of nanostructures. Heating of gold nanoparticles in non-invasive radiofrequency electric field (RF-EF) is a promising and unique technique for cancer hyperthermia. However, because of differences between particles (i.e. their surface chemistry and dispersion medium) and between RF-EF sources, the research community has not reached a consensus yet. Here, we report the results of investigations on heating of gold nanoparticles and gold nanorods under RF-EF and feasibility of in-vitro cancer hyperthermia. The heating experiments were performed to investigate the role of particle shape and surface chemistry (CTAB, citrate and PEG molecules). In-vitro hyperthermia was performed on human pancreatic cancer cell (MIA Paca-2) with PEG-coated GNPs and GNRs at concentrations that were found non-toxic based on the results of cytotoxicity assay. Application of RF-EF on cells treated with PEG-GNPs and PEG-GNRs proved highly effective in killing cells.

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Section: Different Types Of Gold Nanostructures Have Found Unique Capmentioning

confidence: 99%

Radiofrequency electric field hyperthermia with gold nanostructures: role of particle shape and surface chemistry

Amini

Rezayat

Gilani

2017

Artificial Cells, Nanomedicine, and Biotechnology

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“…AuNPs were synthesised based on our previous report using Turkevich method [21, 22]. Briefly, under vigorous stirring 100 ml of gold (III) chloride trihydrate (HAuCl 4 .…”

Section: Methodsmentioning

confidence: 99%

“…AuNPs were synthesised based on our previous report using Turkevich method [21,22] The average size of synthesised AuNPs was calculated by DigitalMicrograph ® and origin 9.1 software using five different transmission electron microscopy (TEM) micrographs and UVvisible spectrum. Also, to determine particles hydrodynamic size, DLS was performed in 25°C with these settings for Au nanoparticles: refractive index (0.1986), and absorption coefficient of 3.2279 cm −1 .…”

Section: Aunps Synthesis and Characterisationmentioning

confidence: 99%

Cross‐linking gold nanoparticles aggregation method based on localised surface plasmon resonance for quantitative detection of miR‐155

Esmaeili-Bandboni

Amini

Faridi‐Majidi

et al. 2018

IET nanobiotechnol.

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MiR-155 plays a critical role in the formation of cancers and other diseases. In this study, the authors aimed to design and fabricate a biosensor based on cross-linking gold nanoparticles (AuNPs) aggregation for the detection and quantification of miR-155. Also, they intended to compare this method with SYBR Green real-time polymerase chain reaction (PCR). Primers for real-time PCR, and two thiolated capture probes for biosensor, complementary with miR-155, were designed. Citrate capped AuNPs (18.7 ± 3.6 nm) were synthesised and thiolated capture probes immobilised to AuNPs. The various concentrations of synthetic miR-155 were measured by this biosensor and real-time PCR method. Colorimetric changes were studied, and the calibration curves were plotted. Results showed the detection limit of 10 nM for the fabricated biosensor and real-time PCR. Also, eye detection using colour showed the weaker detection limit (1 µM), for this biosensor. MiR-133b as the non-complementary target could not cause a change in both colour and UV-visible spectrum. The increase in hydrodynamic diameter and negative zeta potential of AuNPs after the addition of probes verified the biosensor accurately fabricated. This fabricated biosensor could detect miR-155 simpler and faster than previous methods.

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“…Furthermore, recent reports have suggested the use of Au-NPs as a multienzyme carrier in ELISA assay to increase its sensitivity and decrease assay time. 92,93 In addition, their plasmon resonance properties can be well manipulated by varying the nanoparticle composition, size, and shape. The surface of metal nanomaterials can be readily functionalized via the formation of self-assembled monolayers (SAM) of biomolecular recognition elements (ligands/biotin, proteins, DNA, antibodies), providing further benets.…”

Section: Protein Detectionmentioning

confidence: 99%

Portable point-of-care diagnostic devices

et al. 2016

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Comparison of Gold Nanoparticle Conjugated Secondary Antibody with Non-Gold Secondary Antibody in an ELISA Kit Model

Cited by 22 publications

References 9 publications

Radiofrequency electric field hyperthermia with gold nanostructures: role of particle shape and surface chemistry

Radiofrequency electric field hyperthermia with gold nanostructures: role of particle shape and surface chemistry

Cross‐linking gold nanoparticles aggregation method based on localised surface plasmon resonance for quantitative detection of miR‐155

Portable point-of-care diagnostic devices

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