Cross‐linking gold nanoparticles aggregation method based on localised surface plasmon resonance for quantitative detection of miR‐155

Esmaeili-Bandboni, Aghil; Amini, Seyed Mohammad; Faridi‐Majidi, Reza; Bagheri, Jamshid; Mohammadnejad, Javad; Sadroddiny, Esmaeil

doi:10.1049/iet-nbt.2017.0174

Cited by 28 publications

(12 citation statements)

References 42 publications

(48 reference statements)

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“…Gold NPs (AuNPs) have been widely evaluated in various biomedical fields such as cancer therapy, drug/gene delivery, biosensors, cell tracking, and regenerative medicine 15 – 17 . AuNPs offer brilliant properties such as biocompatibility, chemical inertness and stability, high surface to volume ratio, easy synthesis, and surface modification.…”

Section: Introductionmentioning

confidence: 99%

Bioengineered 3D nanocomposite based on gold nanoparticles and gelatin nanofibers for bone regeneration: in vitro and in vivo study

Samadian

Khastar

Ehterami

et al. 2021

Sci Rep

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The main aim of the present study was to fabricate 3D scaffold based on poly (l-lactic acid) (PLLA)/Polycaprolactone (PCL) matrix polymer containing gelatin nanofibers (GNFs) and gold nanoparticles (AuNPs) as the scaffold for bone tissue engineering application. AuNPs were synthesized via the Turkevich method as the osteogenic factor. GNFs were fabricated by the electrospinning methods and implemented into the scaffold as the extracellular matrix mimicry structure. The prepared AuNPs and Gel nanofibers were composited by PLLA/PCL matrix polymer and converted to a 3D scaffold using thermal-induced phase separation. SEM imaging illustrated the scaffold's porous structure with a porosity range of 80–90% and a pore size range of 80 to 130 µm. The in vitro studies showed that the highest concentration of AuNPs (160 ppm) induced toxicity and 80 ppm AuNPs exhibited the highest cell proliferation. The in vivo studies showed that PCL/PLLA/Gel/80ppmAuNPs induced the highest neo-bone formation, osteocyte in lacuna woven bone formation, and angiogenesis in the defect site. In conclusion, this study showed that the prepared scaffold exhibited suitable properties for bone tissue engineering in terms of porosity, pore size, mechanical properties, biocompatibility, and osteoconduction activities.

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Section: Introductionmentioning

confidence: 99%

Bioengineered 3D nanocomposite based on gold nanoparticles and gelatin nanofibers for bone regeneration: in vitro and in vivo study

Samadian

Khastar

Ehterami

et al. 2021

Sci Rep

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“…However, the non-functionalized AuNPs were aggregated at the low concentration of NaCl (0.1 M), and the solution's color changed from red to blue. The phosphates of the probe nucleotides increased the negative surface charge of the AuNPs, leading to the repulsion interaction of the AuNPs and the increased stability of the NPs [30].…”

Section: Functionalization Of Aunpsmentioning

confidence: 99%

Development and comparison of cross-linking and non-crosslinking probe-gold nanoparticle hybridization assays for direct detection of unamplified bovine viral diarrhea virus-RNA

2021

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Background Bovine viral diarrhea virus (BVDV) is a major economic disease that has been spread in most countries. In addition to vaccination, one of the main ways to control the disease and prevent it from spreading is to detect and cull infected animals, especially those with persistent infection (PI). We developed and compared two colorimetric biosensor assays based on probe-modified gold nanoparticles (AuNPs) to detect BVDV. Specific probes were designed to detect the 5′ untranslated region of BVDV-RNA. The thiolated probes were immobilized on the surface of the AuNPs. Two methods of cross-linking (CL) and non-crosslinking (NCL) probe-AuNPs hybridization were developed and compared. Results The hybridization of positive targets with the two probe-AuNPs formed a polymeric network between the AuNPs which led to the aggregation of nanoparticles and color change from red to blue. Alternatively, in the NCL mode, the hybridization of complementary targets with the probe-AuNPs resulted in the increased electrostatic repulsion in nanoparticles and the increased stabilization against salt-induced aggregation. The CL and NCL assays had detection limits of 6.83 and 44.36 ng/reaction, respectively. Conclusion The CL assay showed a higher sensitivity and specificity; in contrast, the NCL assay did not require optimizing and controlling of hybridization temperature and showed a higher response speed. However, both the developed methods are cost-effective and easy to perform and also could be implemented on-site or in local laboratories in low-resource countries.

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“…However, the nonfunctionalized AuNPs were aggregated at the low concentration of NaCl (0.1 M), and the solution's color changed from red to blue. The phosphates of the probe nucleotides increased the negative surface charge of the AuNPs, leading to the repulsion interaction of the AuNPs and the increased stability of the NPs [30].…”

Section: Functionalization Of Aunpsmentioning

confidence: 99%

Development and Comparison of Cross-Linking and Non-Crosslinking Probe-Gold Nanoparticle Hybridization Assays for Direct Detection of Unamplified Bovine Viral Diarrhea Virus-RNA

Heidari

Rezatofighi

Rastegarzadeh

2020

Preprint

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Background: Bovine viral diarrhea virus (BVDV) is a major economic disease that has been spread in most countries. In addition to vaccination, one of the main ways to control the disease and prevent it from spreading is to detect and cull infected animals, especially those with persistent infection (PI). We developed and compared two colorimetric biosensor assays based on probe-modified gold nanoparticles (AuNPs) to detect BVDV. Specific probes were designed to detect the 5ʹ untranslated region of BVDV-RNA. The thiolated probes were immobilized on the surface of the AuNPs. Two methods of cross-linking (CL) and non-crosslinking (NCL) probe-AuNPs hybridization were developed and compared. Results: The hybridization of positive targets with the two probe-AuNPs formed a polymeric network between the AuNPs which led to the aggregation of nanoparticles and color change from red to blue. Alternatively, in the NCL mode, the hybridization of complementary targets with the probe-AuNPs resulted in the increased electrostatic repulsion in nanoparticles and the increased stabilization against salt-induced aggregation. The CL and NCL assays had detection limits of 6.83 and 44.36 ng/reaction, respectively. Conclusion: The CL assay showed a higher sensitivity and specificity; in contrast, the NCL assay did not require optimizing and controlling of hybridization temperature and showed a higher response speed. However, both the developed methods are cost-effective and easy to perform and also could be implemented on-site or in local laboratories in low-resource countries.

show abstract

Cross‐linking gold nanoparticles aggregation method based on localised surface plasmon resonance for quantitative detection of miR‐155

Cited by 28 publications

References 42 publications

Bioengineered 3D nanocomposite based on gold nanoparticles and gelatin nanofibers for bone regeneration: in vitro and in vivo study

Bioengineered 3D nanocomposite based on gold nanoparticles and gelatin nanofibers for bone regeneration: in vitro and in vivo study

Development and comparison of cross-linking and non-crosslinking probe-gold nanoparticle hybridization assays for direct detection of unamplified bovine viral diarrhea virus-RNA

Development and Comparison of Cross-Linking and Non-Crosslinking Probe-Gold Nanoparticle Hybridization Assays for Direct Detection of Unamplified Bovine Viral Diarrhea Virus-RNA

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