Comparison of Filtration and Impingement for Sampling Airborne Endotoxin

Zucker, Barbara; Draz, A.M.; Müller, Wolfgang

doi:10.1016/s0021-8502(99)00556-x

Cited by 13 publications

(9 citation statements)

References 7 publications

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“…The low number of gram-negative bacteria isolated in the air samples did not imply that the air was free from their endotoxins because the activity of endotoxins is not terminated with the degradation of the bacteria (Zucker et al, 2000b), thus posing a health risk for both animals and farm workers.…”

Section: Resultsmentioning

confidence: 99%

Qualitative structure of airborne bacteria and fungi in dairy barn and nearby environment

Matković¹,

Vučemilo²,

Vinković³

et al. 2007

CZECH J ANIM SCI

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ABSTRACT:The study was conducted in a dairy barn and nearby environment to determine the level of air bacterial and fungi contamination. Measurements were performed in morning, noon and evening sampling periods once a week during two autumn months inside the barn and in a 25 m distant barn environment. A Merck MAS-100 air sampler was employed with different medium and incubation combinations for the capture and identification of bacteria and fungi. The results of the study showed a statistically significant difference (P < 0.05) between the bacterial and fungi counts measured inside and outside the barn, whereby total outside bacterial count was by 97.4% to 98.0% lower, and total outside fungi count by 85.2% to 99.4% lower than the respective indoor counts in various sampling periods. In 125 analyzed colonies, gram-positive bacteria, especially those of the genus Staphylococcus and Streptococcus, were most commonly identified in the air samples from both inside and outside the barn. Gram-negative bacteria were present at a low rate, predominated by enterobacteria and by the genus Moraxella and Pseudomonas. Nine mould genera were identified in 325 fungi colonies, predominated by the genus Aspergillus, Penicillium, Rhizopus and yeasts, both in the barn and in the nearby environment.

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Section: Resultsmentioning

confidence: 99%

Qualitative structure of airborne bacteria and fungi in dairy barn and nearby environment

Matković¹,

Vučemilo²,

Vinković³

et al. 2007

CZECH J ANIM SCI

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“…the air to be tested is led through pyrogen-free water which is then tested in the lAl. the higher pyrogen retrieval by impingement when compared to filtration, possibly due to the incomplete eluation of the sample from the filters, was demonstrated by Zucker et al (2000). A new approach of measuring the integral inflammatory activity in air samples in different environments by WBt was reported by Kindinger et al, 2005.…”

Section: Airborne Pyrogensmentioning

confidence: 99%

Development, validation and applications of the monocyte activation test for pyrogens based on human whole blood

Schindler

Aulock

Daneshian

et al. 2009

ALTEX

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“…This method may inaccurately represent the quantity of allergens and pyrogens entering the lungs (Platts‐Mills et al., 1995). In recent studies that have targeted airborne endotoxin, the most widely adapted practice is to collect known quantities of air through membrane filters, although standard AGI‐30 liquid impingers (Ace Glass Inc., Vineland, NJ, USA) have been used and appear to be more efficient when compared with other particle samplers (Duchaine et al., 2001; Zucker et al., 2000).…”

Section: Introductionmentioning

confidence: 99%

Particle size distributions and concentrations of airborne endotoxin using novel collection methods in homes during the winter and summer seasons

Kujundzic¹,

Hernandez²,

Miller

2006

Indoor Air

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This study compared sampling methods for airborne endotoxin, a potent and nonspecific immune system stimulant which can induce negative health responses. The data from this study showed that swirling liquid impingers and the micro-orifice uniform deposit impactor (MOUDI) recovered significantly more airborne endotoxin than the more widely adapted method of collecting airborne endotoxin on membrane filters, when collection methods were applied in realistic settings (homes). The MOUDI measured the particle size distribution of airborne endotoxin, which can be useful for determining endotoxin respiratory toxicity and its health effects.

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Comparison of Filtration and Impingement for Sampling Airborne Endotoxin

Cited by 13 publications

References 7 publications

Qualitative structure of airborne bacteria and fungi in dairy barn and nearby environment

Qualitative structure of airborne bacteria and fungi in dairy barn and nearby environment

Development, validation and applications of the monocyte activation test for pyrogens based on human whole blood

Particle size distributions and concentrations of airborne endotoxin using novel collection methods in homes during the winter and summer seasons

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