Comparison of fertility between the F1, F2 and anther derived lines in the crosses of indica/japonica and japonica/indica in rice (Oryza sativa L.)

Narasimman, R.; Rangasamy, S.

doi:10.1007/bf00023504

Cited by 6 publications

(2 citation statements)

References 14 publications

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“…By understanding the inheritance patterns of anther culture response, breeders can overcome genotype differences by crossing highly responsive to non responsive genotypes and can, to some extent, predict the level of response of the hybrids (Hou et al, 1994). Significant genotypic differences have been observed for callus induction and plant regeneration among the different hybrids of indica and japonica (Narasimman & Rangasamy, 1993).…”

Section: Introductionmentioning

confidence: 99%

Anther culture performance in selected high yielding indica (of Sri Lanka) and japonica rice varieties and their inter sub-specific hybrids

Herath

Bandara

2011

J. Natn. Sci. Foundation Sri Lanka

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The ability to improve anther culture response of some high yielding commercially grown indica rice varieties by crossing them with a high anther culture responding japonica rice variety was studied. Performance of japonica rice variety Hu lo tao, indica rice varieties Bg 90-2, Bg 379-2, Bg 94-1 and their F1 hybrids and F2 plants were investigated for callus induction and plant regeneration. Panicles were stored at 8 0 C for 14 days as the cold pre-treatment and anthers were cultured in Chu's N6 medium with 5% (w/v) sucrose, 2.0 mg L-1 2,4dichloro phenoxy acetic acid (2,4 D) and 0.5 mg L-1 Kinetin. The cultures were kept in the dark at 28 + 2 o C for callus induction. Induced calli of 1-2 mm diameter were transferred to half strength Murashige and Skoog (MS) medium supplemented with 2.0 mg L-1 Kinetin and 0.5 mg L-1 naphthalene acetic acid (NAA). Of the parental varieties, Hu lo tao is more responsive to anther culture. F2 plants, compared to F1 hybrids and parental rice varieties, had greater callus induction frequencies and plant regeneration. Calli from F1 hybrids and F2 plants of Bg 90-2 × Hu lo tao showed the highest green plant regeneration (63.3% and 69.7% respectively). The regeneration of F1hybrids and F2 plants were higher than in the parents. According to the results, it is possible to improve the anther culture performance of F1 hybrids and F2 plants by crossing high yielding indica rice varieties with high anther culture responding japonica varieties.

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Section: Introductionmentioning

confidence: 99%

Anther culture performance in selected high yielding indica (of Sri Lanka) and japonica rice varieties and their inter sub-specific hybrids

Herath

Bandara

2011

J. Natn. Sci. Foundation Sri Lanka

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“…Several other researchers have also noticed a decline in androgenesis in the following order: Japonica > Japonica × Indica > Indica (Chen & Lin 1976;Tsai & Lin 1977;Chaleff 1978;Miah et al 1985). Both callus induction and green plant regeneration have varied considerably depending on the specific cultivars used to construct the hybrids (Narasimman & Rangasamy 1993).…”

Section: Caulogenesis Inducementmentioning

confidence: 99%

In vitro studies to produce double haploid in Indica hybrid rice

et al. 2011

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The aim of this investigation was to improve in vitro the technique of production of double haploid in Indica hybrid rice by combining anther culture, hormone shock and doubling chromosome. It was discussed how to avoid somaclonal variation during culturing and to reduce the time of this process. The anthers of KDML 105 × SPR 1 (Indica × Indica) were cultured in Linsmaier and Skoog (LS) medium, which contained nutrients, growth regulators [(2,4,-dichlorophenoxy acetic acid (2,4-D) and naphthalene acetic acid (NAA)] and organic compounds, and then subcultured by inducing embryo-like structure (ELS) LS media. During 4 weeks used LS media supplemented with 10 µM KNO3 + 2 mg/L 2,4-D + 2 mg/L NAA + 20% coconut water + 1 mg/L of activated charcoal had induced high embryogenic frequent callus with length of 4-5 mm. The supplementation of 0.2 g/L colchicine and 100 µM 2,4-D was the most efficient in LS media. Over 70% of viable double haploid ELS were produced in 8 weeks and subcultured only twice compared with conventional anther which takes more than 12 weeks. This new technique can therefore be applied to rice in order in shorten time to produce higher number of double haploid plantlets.

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Doubled Haploid Breeding in Cereals

Raina

1997

Plant Breeding Reviews

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Comparison of fertility between the F1, F2 and anther derived lines in the crosses of indica/japonica and japonica/indica in rice (Oryza sativa L.)

Cited by 6 publications

References 14 publications

Anther culture performance in selected high yielding indica (of Sri Lanka) and japonica rice varieties and their inter sub-specific hybrids

Anther culture performance in selected high yielding indica (of Sri Lanka) and japonica rice varieties and their inter sub-specific hybrids

In vitro studies to produce double haploid in Indica hybrid rice

Doubled Haploid Breeding in Cereals

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