In vitro studies to produce double haploid in Indica hybrid rice

Premvaranon, Piyachai; Vearasilp, Suchada; Thanapornpoonpong, Sa-nguansak; Karladee, Dumnern; Gorinstein, Shela

doi:10.2478/s11756-011-0129-8

Cited by 20 publications

(10 citation statements)

References 24 publications

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“…There are many factors like length of culture periods, genotype and nature of explant, which could influence the stability of the tissue cultured plants (Premvaranon et al 2011). So assessment of genetic stability of in vitro regenerated plantlets is highly significant for further studies.…”

Section: Resultsmentioning

confidence: 99%

Micropropagation and analysis of genetic stability in regenerated plantlets of Ocimum canum Sims.

Saha

Roy

Sengupta

et al. 2014

Ind J Plant Physiol.

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An efficient and improved plant regeneration protocol has been developed from nodal explants of Ocimum canum Sims., a medicinally important herbaceous plant species belonging to the family Lamiaceae. Murashige and Skoog (MS) basal medium supplemented with 0.5 mg l -1 N 6 -benzyladenine (BA) showed maximum percentage of shoot multiplication (91.1 %) with 5.32 shoots per explant. Rooting of shoots occurred on 1/2 MS medium supplemented with 1.0 mg l -1 indole-3-butyric acid (IBA). Well-developed plantlets, transferred to plastic pots containing sand, soil and compost (2:2:1) showed 80.9 % survival rate under field condition. The genetic fidelity of in vitro raised field-grown plants to the donor plant was ascertained from histological, cytological and two PCR based molecular markers, i.e., random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR). Histological study showed direct organogenesis on epidermal and sub epidermal layer, where as cytological studies showed no visible anomalies within its chromosome compliments. Both RAPD and ISSR analysis revealed high degree of monomorphism, similar to those of the mother plants. This method of in vitro clonal propagation of O. canum Sims. may, therefore, could be an avenue of its sustainable commercial exploitation and conservation.

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Section: Resultsmentioning

confidence: 99%

Micropropagation and analysis of genetic stability in regenerated plantlets of Ocimum canum Sims.

Saha

Roy

Sengupta

et al. 2014

Ind J Plant Physiol.

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show abstract

“…1E). There are many factors like length of culture periods, genotype and nature of explant, which could influence the stability of the tissue cultured plants (Vendrame et al 1999;Premvaranon et al 2011). So assessment of genetic stability of in vitro regenerated plantlets is highly significant for further studies.…”

Section: Resultsmentioning

confidence: 99%

Micropropagation and assessment of genetic stability in Celastrus paniculatus: An endangered medicinal plant

2013

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A highly efficient protocol for in vitro regeneration of an indigenous, endangered medicinal plant Celastrus paniculatus was achieved using nodal explants. Murashige and Skoog (MS) basal medium supplemented with 0.5 mg/L 6-benzylaminopurine (BAP) and 0.1 mg/L naphthaleneacetic acid (NAA) showed maximum percentage of shoot multiplication (83.4%) with 8.2 shoots/explants. Maximum rooting of 73.3% with 4.8 roots/shoot was achieved on half-strength MS media supplemented with 0.5 mg/L indole-3-acetic acid (IAA) and the percentage of survival was 91% after acclimatization. Random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) marker study confirmed genetic stability for in vitro raised explants by showing 100% monomorphism. High multiplication rate associated with genetic stability ensure the efficacy of the present in vitro clonal propagation protocol of this important medicinal plant species.

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“…There are many factors like length of culture periods, genotype and nature of explants, which could influence the stability of the tissue cultured plants (Vendrame et al 1999;Premvaranon et al 2011). So assessment of genetic stability of in vitro regenerated plantlets is highly significantly for commercialization.…”

Section: Assessment Of Genetic Uniformity Of In Vitro-raised Plantsmentioning

confidence: 99%

Plant regeneration from leaf explants of Aloe barbadensis Mill. and genetic fidelity assessment through DNA markers

Sahoo

Rout

2014

Physiol Mol Biol Plants

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An efficient plant regeneration protocol was developed from leaf explants of Aloe barbadensis Mill on Murashige and Skoog's (MS) medium supplemented with 2.0 mg/l 6-benzyladenine (BA) or Kinetin (Kn), 0.25-0.5 mg/l NAA (1-napthalene acetic acid) and 3 % (w/v) sucrose within 4 weeks of culture. The maximum number of shoot buds were obtained on MS medium supplemented with 2.0 mg/l BA, 0.5 mg/l NAA, 40 mg/l Ads (adenine sulphate) within 4-6 weeks of subculture. Inclusion of 0.25-0.50 mg/l gibberellic acid into the medium, the shoot buds became elongated. Repeated subculture on regeneration medium induces higher rate of shoot regeneration. The root induction from excised microshoots was achieved on half-strength MS medium supplemented with 0.25-1.0 mg/l NAA or indole-3-butyric acid (IBA) and 2 % (w/v) sucrose. Maximum percentage of rooting was achieved on medium having 0.5 mg/l NAA with 3 % (w/v) sucrose. About 80 % of in vitro raised plantlets were hardened in the greenhouse and successfully established in the soil. Both Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeat (ISSR) markers were used to detect the variability among the regenerated plants developed in vitro. The results showed that there was no polymorphism among the regenerated plantlets. This study will help for propagation of quality planting material of Aloe barbadensis for commercialization.

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In vitro studies to produce double haploid in Indica hybrid rice

Cited by 20 publications

References 24 publications

Micropropagation and analysis of genetic stability in regenerated plantlets of Ocimum canum Sims.

Micropropagation and analysis of genetic stability in regenerated plantlets of Ocimum canum Sims.

Micropropagation and assessment of genetic stability in Celastrus paniculatus: An endangered medicinal plant

Plant regeneration from leaf explants of Aloe barbadensis Mill. and genetic fidelity assessment through DNA markers

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