Micropropagation and analysis of genetic stability in regenerated plantlets of Ocimum canum Sims.

Saha, Soumen; Roy, Sudip; Sengupta, Chandan; Ghosh, Parthadeb

doi:10.1007/s40502-014-0093-8

Cited by 15 publications

(8 citation statements)

References 21 publications

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“…3). Our results demonstrate that axillary shoot proliferation minimizes the chance of instability, consistent with previous reports 17,67,68 . This is the first report of genetically sustainable micropropagation in Magnolia plants.…”

Section: Shoot Bud Proliferationsupporting

confidence: 93%

An efficient micropropagation protocol for an endangered ornamental tree species (Magnolia sirindhorniae Noot. & Chalermglin) and assessment of genetic uniformity through DNA markers

Cui

Deng

Zheng

et al. 2019

Sci Rep

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Magnolia sirindhorniae Noot. & Chalermglin is an endangered species with high ornamental and commercial value that needs to be urgently protected and judiciously commercialized. In this study, a protocol for efficient regeneration of this species is standardized. The lateral buds of the M. sirindhorniae plant were used as an explant. Half-strength Murashige and Skoog (MS) medium supplemented with 2.0 mg/L 6-benzyladenine (BA), 0.1 mg/L α-naphthaleneacetic acid (NAA), and 2.0 mg/L gibberellic acid (GA 3 ) was found to be the optimal medium for shoot induction. The maximum shoot multiplication rate (310%) was obtained on Douglas-fir cotyledon revised medium (DCR) fortified with 0.2 mg/L BA, 0.01 mg/L NAA, and additives. The half-strength DCR medium supplemented with 0.5 mg/L NAA and 0.5 mg/L indole-3-butyric acid (IBA) supported the maximum rate (85.0%) of in vitro root induction. After a simple acclimatization process, the survival rate of plantlets in a substrate mixture of sterile perlite and peat soil (1:3; v/v ) was 90.2%. DNA markers were used for assessment of genetic uniformity, confirming the genetic uniformity and stability of regenerated plants of M. sirindhorniae . Thus, the described protocol can safely be applied for large scale propagation of this imperative plant.

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Section: Shoot Bud Proliferationsupporting

confidence: 93%

An efficient micropropagation protocol for an endangered ornamental tree species (Magnolia sirindhorniae Noot. & Chalermglin) and assessment of genetic uniformity through DNA markers

Cui

Deng

Zheng

et al. 2019

Sci Rep

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“…Utility of molecular analysis of in vitro regenerated plants has been well documented by many workers ( Bhatia et al, 2011 , Saha et al, 2010 , Saha et al, 2012 , Saha et al, 2014a , Saha et al, 2014b ). Genetic fidelity of micropropagated plants has immense practical utility and commercial implications.…”

Section: Resultsmentioning

confidence: 99%

RAPD and ISSR based evaluation of genetic stability of micropropagated plantlets of Morus alba L. variety S-1

et al. 2016

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Plant regeneration through rapid in vitro clonal propagation of nodal explants of Morus alba L. variety S-1 was established along with genetic stability analysis of regenerates. Axillary shoot bud proliferation was achieved on Murashige and Skoog (MS) medium in various culture regimes. Highest number of shoots (5.62 ± 0.01), with average length 4.19 ± 0.01 cm, was initially achieved with medium containing 0.5 mg/l N6-benzyladenine (BA) and 3% sucrose. Repeated subculturing of newly formed nodal parts after each harvest up to sixth passage, yielded highest number of shoots (about 32.27) per explants was obtained after fourth passage. Rooting of shoots occurred on 1/2 MS medium supplemented with 1.0 mg/1 Indole-3-butyric acid (IBA). About 90% (89.16) of the plantlets transferred to the mixture of sand:soil:organic manure (2:2:1) in small plastic pots acclimatized successfully. Genetic stability of the discussed protocol was confirmed by two DNA-based fingerprinting techniques i.e. RAPD (random amplified polymorphic DNA) and ISSR (inter-simple sequence repeat). This protocol can be used for commercial propagation and for future genetic improvement studies.

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“…Morphogenic responses, such as shoot regeneration, are observed by the application of cytokinins either alone (Razaq et al, 2012;Saha et al, 2014;Sadeghi et al, 2015), or in combination with auxins (Upadhyay et al, 2015;Savitikadi et al, 2020). Both PGRs have a synergistic effect on morphogenic responses, which has been reported in other medicinal species such as Rhodiolarosea (Dimitrov et al, 2003).…”

Section: In Vitro Culture Shoot Development and Multiplication Indexmentioning

confidence: 89%

Indirect Shoot Regeneration Using 2,4-D Induces Somaclonal Variations in Cinchona officinalis

2021

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Cinchona officinalis is an important species from the Andean cloud forest that has a low regeneration rate in natural populations. In vitro regeneration of C. officinalis has been successfully established but somaclonal variation was not evaluated. The regeneration pathway and the number of subcultures on somaclonal variation were evaluated using six ISSR primers that amplified 58 loci of Inter Simple Sequence Repeats (ISSR). A dendrogram based on Jaccard´s genetic distance between the subcultures and the donor plant was produced. The results show that indirect shoot regeneration induces somaclonal variation, in the presence 2,4-Dichlorophenoxyacetic acid (2,4-D) in combination with kinetin and 6-Benzylaminopurine (BAP). In combination with 1-Naphthaleneacetic acid (NAA) or with Indole-3-butyric acid (IBA), BAP produces genetically stable explants. The highest proliferation rate was achieved using BAP and IBA. The present research study suggests avoiding the use of 2,4-D when C. officinalis is propagated for reintroduction and restoration projects.

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Micropropagation and analysis of genetic stability in regenerated plantlets of Ocimum canum Sims.

Cited by 15 publications

References 21 publications

An efficient micropropagation protocol for an endangered ornamental tree species (Magnolia sirindhorniae Noot. & Chalermglin) and assessment of genetic uniformity through DNA markers

An efficient micropropagation protocol for an endangered ornamental tree species (Magnolia sirindhorniae Noot. & Chalermglin) and assessment of genetic uniformity through DNA markers

RAPD and ISSR based evaluation of genetic stability of micropropagated plantlets of Morus alba L. variety S-1

Indirect Shoot Regeneration Using 2,4-D Induces Somaclonal Variations in Cinchona officinalis

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