Doubled Haploid Breeding in Cereals

Raina, Satish

doi:10.1002/9780470650097.ch5

Cited by 17 publications

(17 citation statements)

References 161 publications

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“…This has been borne out in more recent studies where reduction of the level of 2,4-D to 0.5 mg/L (and omission of picloram altogether) in the callus induction medium appeared eventually to be the most effective for anther response, even though confounding effects from modifications made to various other culture media components did not allow a clear 'cause and effect' relationship to be established between growth regulators and anther response (Bishnoi et al 2000). A lower level of 2,4-D (0.5 mg/L) in combination with the milder auxin NAA (2.5 mg/L), and kinetin (0.5 mg/L) have been used effectively to induce callus from several indica varieties (Shahnewaz et al 2003;Shahnewaz and Bari 2004) and have been observed to be desirous for the overall efficiency of green plant regeneration from the induced callus (Raina 1997). A combination of the same three growth regulators in somewhat similar proportions (2,4-D 1 mg/L, NAA 2.5 mg/L, and kinetin 0.5 mg/L) have had a positive influence on callus induction from cultured anthers of the interspecific hybrid, O. sativa 9 O. rufipogon, while the regeneration frequency of calli produced in the presence of both auxins was also reported to be higher (Rout and Sarma 1991).…”

Section: Plant Growth Regulatorsmentioning

confidence: 98%

“…It has been suggested that liquid culture systems may provide microspores and calli with greater access to nutrients and hormones while dispersing more rapidly the toxic substances released from dying or dead anthers. The tendency for the rice anthers to sink in liquid media and rapidly lose viability has been a major reason for avoidance of liquid culture conditions by many (Raina 1997). Efforts to keep anthers afloat and viable in liquid culture, by adding substances such as Ficoll that increases buoyancy, has been reasonably successful.…”

Section: Plant Growth Regulatorsmentioning

confidence: 98%

“…The potential of anther culture in producing marker-free transgenic rice, a vexed issue with the consumer, has also been recognized (Zhu et al 2007). However, most developments have occurred in temperate japonica cultivars while success with tropical indica types has remained poor (Kush and Brar 2002;Raina 1997). As a result, anther culture is now used as a supplementary breeding tool in japonica rice (Brar and Kush 2006;Kush and Brar 2002), but the potential of the technique for indica rice breeding is yet to be fully exploited (He et al 2006) in spite of an initial report of the release of a salt tolerant indica variety through anther culture breeding (Senadhira et al 2002).…”

Section: Introductionmentioning

confidence: 97%

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Indica rice anther culture: can the impasse be surpassed?

Silva

2009

Plant Cell Tiss Organ Cult

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During the past two decades numerous papers have been published on anther culture of rice. These studies clearly indicate that while anther culture is a technique that can be adopted for breeding japonica rice, it being a useful adjunct in indica rice breeding is still some way away. The main reasons why anther culture cannot be utilized for indica rice breeding is analyzed, and aspects that may be manipulated to achieve progress are presented in this review. The two stages of rice anther culture, callus induction and green plant regeneration, are genetically determined traits that show quantitative inheritance. Indica rice is known to have a recalcitrant genetic background that supports these traits poorly. While improvement of the genetic background through recombination or gene transfer remains possible, manipulation of culture media, particularly the nitrogen and carbon sources, has brought about substantial improvements in indica rice anther culture. Adjustments to pre-and post-culture conditions, that include application of various stresses on anthers before and after culture, also have had beneficial effects. The importance of reducing the tissue culture phase to achieve direct embryogenesis is discussed with special reference to improving green plant regeneration potential. The necessity to understand the processes involved in microspore embryogenesis is highlighted in order to support empirical knowledge and achieve a breakthrough in technology. In this regard, rice genome sequence information may be leveraged to elucidate functions of genes involved in microspore embryogenesis.

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Section: Plant Growth Regulatorsmentioning

confidence: 98%

Section: Plant Growth Regulatorsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 97%

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Indica rice anther culture: can the impasse be surpassed?

Silva

2009

Plant Cell Tiss Organ Cult

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“…Doubled haploid (DH) is a genotype produced when haploid cells undergo the process of chromosome doubling (Ouyang et al, 1973;Picard and De Buyser, 1973). Doubled haploid production requires induction of haploids and doubling of chromosomes (Snape, 1989;Raina, 1997).…”

Section: Doubled Haploids: Origin and Productionmentioning

confidence: 99%

Recent advances and application of doubled haploids in wheat breeding

Tadesse¹,

Inagaki²,

Tawkaz³

et al. 2012

Afr. J. Biotechnol.

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Genetic improvement to develop varieties with high yield potential and resistance/tolerance to abiotic and biotic stresses with acceptable end use qualities is the most viable and environment friendly option to increase wheat yield in a sustainable fashion. In vitro haploid production followed by chromosome doubling greatly enhances the production of complete homozygous wheat lines in a single generation and increases the precision and efficiency of selection process in wheat breeding. It also enables the detection of linkage and gene interactions, estimate genetic variance and the number of genes for quantitative characteristics, produce genetic translocations, substitutions and chromosome addition lines, and facilitate genetic transformation and mutation studies. Wheat cultivars developed from doubled haploids using anther-culture and maize induction systems have been released for cultivation in both developed and developing countries. In this review, the origin and production of haploids, techniques in anther-culture and wheat x maize wide crosses, and their application in wheat breeding are summarized.

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“…Accordingly, rice anther culture has been intensively investigated. However, despite significant improvements, its practical application remains limited [1]. Nevertheless, anther culture has been widely integrated into rice breeding programs, allowing rapid production of homozygous double haploid lines from F1 hybrids and incorporation of new genes into breeding materials.…”

Section: Introductionmentioning

confidence: 99%

Effect of Maltose Concentration on Plant Regeneration of Anther Culture with Different Genotypes in Rice (<i>Oryza sativa </i>L.)

Park¹,

Ubaidillah²,

Kim³

2013

AJPS

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This study describes the impact of different concentrations of maltose on plant regeneration of anther culture for five genotypes of rice (Oryza sativa). N 6 medium was used for calli induction, while N 6 medium supplemented with different concentrations of maltose, 2.0 mg/L NAA and 0.5 mg/L kinase was used for plant regeneration. The result showed that during the initial stages of calli induction the anther cultures had varying rates of calli formation among genotypes, with the best frequency being observed for Dreami2/CaMsrB2-8-DH-1 with a calli frequency of 27.8%. Different genotypes of rice cultured in regeneration media showed varying plantlet regeneration on media supplemented with different concentrations of maltose, with low concentrations (0.04 g/L) leading to low frequency regeneration plantlet but high green plant production. Indeed, when Dreami2/CaMsrB2-8-DH-2 and Dreami2/CaMsrB2-8-DH-5 were cultivated under these conditions, 100% green plants were observed. Another genotype also showed a small rate of albino frequency in response to the lowest concentration of maltose, while increased maltose concentrations resulted in increased rates of albino plants. Overall, the results of this study should facilitate establishment of an efficient plant regeneration system from anther culture in rice.

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Doubled Haploid Breeding in Cereals

Cited by 17 publications

References 161 publications

Indica rice anther culture: can the impasse be surpassed?

Indica rice anther culture: can the impasse be surpassed?

Recent advances and application of doubled haploids in wheat breeding

Effect of Maltose Concentration on Plant Regeneration of Anther Culture with Different Genotypes in Rice (<i>Oryza sativa </i>L.)

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Doubled Haploid Breeding in Cereals

Cited by 17 publications

References 161 publications

Indica rice anther culture: can the impasse be surpassed?

Indica rice anther culture: can the impasse be surpassed?

Recent advances and application of doubled haploids in wheat breeding

Effect of Maltose Concentration on Plant Regeneration of Anther Culture with Different Genotypes in Rice (&lt;i&gt;Oryza sativa &lt;/i&gt;L.)

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Product

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Effect of Maltose Concentration on Plant Regeneration of Anther Culture with Different Genotypes in Rice (<i>Oryza sativa </i>L.)