Comparison of ELISA and PCR assays for the diagnosis of porcine cysticercosis

Ramahefarisoa, Rondro Mamitiana; Rakotondrazaka, Mahenintsoa; Jambou, Ronan; Carod, Jean-François

doi:10.1016/j.vetpar.2010.05.002

Cited by 24 publications

(14 citation statements)

References 14 publications

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“…Therefore, molecular diagnostics have been considered for validation of macroscopic diagnosis of ambiguous lesions as these tests were reported to be highly specific and sensitive (Gonzalez et al 2006;Sato et al 2006;Chiesa et al 2010). Recently, Ramahefarisoa et al (2010) evaluated the performance of PCR test targeting mitochondrial cytochrome oxidase gene for ante-mortem diagnosis of porcine cysticercosis. Hence, in the present study PCR test was used to detect the metacestode of T. solium and also to validate the meat inspection results, as it involves simple and inexpensive method of sample processing besides facilitating collateral confirmation with high specificity and sensitivity.…”

Section: Discussionmentioning

confidence: 99%

PCR test for detecting Taenia solium cysticercosis in pig carcasses

Sreedevi

Hafeez

Kumar

et al. 2011

Trop Anim Health Prod

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Polymerase chain reaction (PCR) test was employed to detect Taenia solium DNA in muscle lesions for validation of the meat inspection results of slaughtered pigs. Two sets of oligonucleotide primers, one targeted against the large subunit rRNA gene (TBR primers) and the other targeted against cytochrome c oxidase subunit 1 gene (Cox1 primers) of T. solium were used in this study. On reactivity in PCR test, the TBR primers and the Cox1 primers yielded products of 286 and 984 bp, respectively, in cysticercosis positive cases. Both the sets of primers were found to be highly specific, since they did not yield any PCR product in negative controls. A total of 225 pig carcasses were screened for cysticercosis by meat inspection, out of which 25 carcasses with visible cysts (16 viable and 9 degenerated cysts) were also confirmed to be positive for cysticercosis in PCR test. However, out of the 35 carcasses with suspected lesions on meat inspection, only two were found to be positive for cysticercosis in PCR test. The detection limits for both the primer sets were analyzed. The TBR primer set could detect up to 10 pg of cysticercus DNA, whereas the Cox1 primer set could detect only up to 1 ng. It is evident from the study that PCR test is an efficient tool for validation of meat inspection results and also to rule out ambiguity in carcass judgment of suspected cases of porcine cysticercosis.

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Section: Discussionmentioning

confidence: 99%

PCR test for detecting Taenia solium cysticercosis in pig carcasses

Sreedevi

Hafeez

Kumar

et al. 2011

Trop Anim Health Prod

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“…Therefore, the performance of PCR and Antibody ELISA assays has been evaluated by the IPM on 67 pig serum samples. Results indicate that ELISA on pig serum samples shows high sensitivity and good specificity while the PCR has a high specificity but a low sensitivity [30]. The B158/B60 Ag-ELISA [27,28,31] was tested in Madagascar on swine sera.…”

Section: Human Cysticercosismentioning

confidence: 99%

Cysticercosis in Madagascar

Carod

Dorny

2020

J Infect Dev Ctries

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Introduction: Cysticercosis (CC) is the most common parasitic disease of the central nervous system. It is endemic in most developing countries where pigs are raised and consumed. An overview of all available data of this parasite in Madagascar is lacking. Methodology: We conducted a literature review, collecting information on published and available literature about cysticercosis in Madagascar between January 1st, 1990 and June 30th, 2020. Results: Out of 858 publications; 61 were included, issued from peer-review indexed journals, non-indexed journals, books, Ministry reports and press releases. In Madagascar, porcine cysticercosis has been reported since 1901; human cysticercosis is highly prevalent with an overall estimated seroprevalence between 7 and 21%. Serological analysis is based on Enzyme-Linked Immunosorbent Assay (ELISA) and Enzyme-linked immunoelectrotransfer blot techniques (EITB) for confirmative testing. Neurocysticercosis (NCC) is the most common pattern of cysticercosis in Madagascar and it is reponsible for pediatric morbidity causing more than 50% of epilepsy cases. Though CT-Scan is now available and tends to be considered the gold standard for NCC diagnosis, it remains unaffordable for most Malagasy patients and implies the proposal of a diagnostic algorithm for physicians. Conclusions: Our review has revealed that human taeniasis and bovine cysticercosis is a considerable burden in Madagascar. A national control program has been developed aiming to decrease the seroprevalence rate from 16 to 10% in 2015. The aim of the country is now to implement a CC control and elimination program. Meanwhile, some massive cysticercosis screenings have been conducted in the capital Antananarivo to drive people's attention on this widespread infection.

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“…Also, PCR and ELISA have been successfully applied for the detection of parasites in meat, [66][67][68][69][70][71] although with the same disadvantages as previously mentioned. For its part, liquid chromatography-mass spectrometry (LC-MS) has been shown to be a powerful tool for isolating proteins from Anisakis.…”

Section: Evaluation Of Contamination By Parasitesmentioning

confidence: 99%

Evaluation of biological contaminants in foods by hyperspectral imaging: A review

Mantilla

Jara

Yimer

2017

International Journal of Food Properties

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Responding to the ever-growing concern about safe foods and security, the food industries are forced to seek an emerging technology capable of detecting and quantifying contaminations, especially those of biological origin. Among the different emerging technologies, hyperspectral imaging is considered a good alternative as it can be easily applied at all steps of the food production process and is a non-destructive technique. This paper reviews targeted analytical applications of hyperspectral imaging in monitoring biological contaminants in food. First, traditional techniques for detection of biological contaminants in foods are presented, where disadvantages for practical applications are highlighted and explained in detail. Second, prominent applications of hyperspectral imaging from the last decade to food safety and quality assessment are reviewed, specifically focusing on both deteriorative and pathogenic microorganisms, microbial toxins, and parasites; whether acting individually or collectively in spoiling food products and/or represent a health risk to the consumers. Finally, relevant current and future challenges, advantages and disadvantages of hyperspectral imaging applications are briefly examined.

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Comparison of ELISA and PCR assays for the diagnosis of porcine cysticercosis

Cited by 24 publications

References 14 publications

PCR test for detecting Taenia solium cysticercosis in pig carcasses

PCR test for detecting Taenia solium cysticercosis in pig carcasses

Cysticercosis in Madagascar

Evaluation of biological contaminants in foods by hyperspectral imaging: A review

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