Comparison of different extraction methods for the determination of podophyllotoxin and 6-methoxypodophyllotoxin in Linum species

Selection methodology with scoring system: Application to Mexican plants producing podophyllotoxin related lignans

“…For the extraction of lignans, we used the ␤-glucosidase extraction protocol (Empt et al, 2000;Kartal et al, 2004). Following the denaturation of the enzyme, two liquid/liquid extractions were performed, resulting in basic fractionation with three phases: chloroform, butanol and water.…”

Induction of apoptosis in leukemia cell lines by Linum persicum and Euphorbia cheiradenia

“…Some species of this genus are native to Iran and have been used traditionally for their anticancer capacity. Studies have shown that Linum species are an important source for podophyllotoxin or related lignans (Kartal et al 2004). Podophyllotoxin is commercially prepared from Podophyllum emodi Wall and there are some interests to evaluate the content of podophyllotoxin and related compounds in different Linum species with respect to a possible use as an alternative source instead of Podophyllum (Canel et al 2000).…”

Optimization of podophyllotoxin extraction method fromLinum albumcell cultures

“…Kartal et al (2004) compared three extraction methods: aqueous, glucosidase and acetone, for PTOX and its derivatives in Linum species, but no significant differences were perceived. Since the enzymatic method needs the expensive β-glucosidase and was not observed to be better than the others, we did not include it in our study.…”

“…Podophyllotoxin (PTOX) is a natural lignan (Figure 1) used in important anticancer drugs including etoposide, teniposide and Etopophos as well as a new drug, CPH 82, for rheumatoid arthritis (Kartal et al, 2004).…”

“…The genus Linum consists of about 230 species, mainly annual or perennial herbs as well some small shrubs, which are distributed all over the world in different habitats (Kartal et al, 2004). In the flora of Iran, Linum is represented by 15 species, three of which are endemic (Sharifinia & Assadi, 2001).…”