Optimization of podophyllotoxin extraction method from<i>Linum album</i>cell cultures

Yousefzadi, Morteza; Sharifi, Mozafar; Chashmi, Najmeh Ahmadian; Behmanesh, Mehrdad; Ghasempour, Alireza

doi:10.3109/13880209.2010.489564

Cited by 29 publications

(9 citation statements)

References 14 publications

(25 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Secoisolariciresinol is the common precursor of all dibenzylbutyrolactol lignans including matairesinol, yatein and aryltetralin lignans ( Figure 2). These subclasses of lignans includes some important bioactive chemicals from which several structural analogues have been derived, studied and employed as pharmaceuticals [24]. Lignans can be converted by intestinal microbiota, under strictly anaerobic condition, to the mammalian lignans, enterodiol (END) and enterolactone (ENL).…”

Section: Lignan Chemistry and Biochemistrymentioning

confidence: 99%

Cereal Lignans, Natural Compounds of Interest for Human Health?

Zanella

Biasiotto

Holm³

et al. 2017

Natural Product Communications

View full text Add to dashboard Cite

Cereals are suggested to be the most important sources of lignan in the diets of western populations. Recent epidemiological studies show that European subpopulations in which the major source of lignans are cereals, display lower disease frequency regarding metabolic and cardiovascular diseases. The biological mechanisms of lignan are several. Beyond their antioxidant and anti-inflammatory actions at nutritional doses some lignans regulate the activity of specific nuclear receptors (NRs), such as the estrogen receptors (ERs), and also NRs that are central switches in glucose and fatty acid metabolism such as PPAR, PPAR and LXRs, highlighting them as selective nuclear receptor modulators (SNRMs). These include enterodiol (END) and enterolactone (ENL), the metabolites produced by the gut microbiota from food lignans. The available knowledge suggests that given some additional research it should be possible to make 'function' claims for a regular intake of lignans-rich foods related to maintaining a healthy metabolism.

show abstract

Section: Lignan Chemistry and Biochemistrymentioning

confidence: 99%

Cereal Lignans, Natural Compounds of Interest for Human Health?

Zanella

Biasiotto

Holm³

et al. 2017

Natural Product Communications

View full text Add to dashboard Cite

show abstract

“…PTOX was extracted from powdered lyophilized cells by sonication in methanol/water (4:1 v/v), as previously described (Yousefzadi et al 2010b). The presence of PTOX in the samples was verified by ESI/MS in positive ion mode ( Supplementary Fig.…”

Section: Ptox Extraction and Quantificationmentioning

confidence: 99%

“…The presence of PTOX in the samples was verified by ESI/MS in positive ion mode ( Supplementary Fig. 1) and the quantification was performed by HPLC following the method of Yousefzadi et al (2010b).…”

Section: Ptox Extraction and Quantificationmentioning

confidence: 99%

Salicylic acid improves podophyllotoxin production in cell cultures of Linum album by increasing the expression of genes related with its biosynthesis

et al. 2010

Self Cite

View full text Add to dashboard Cite

Treatment of Linum album cell cultures with 10 μM salicylic acid (SA) for 3 days improved podophyllotoxin (PTOX) production up to 333 μg/g dry weight (DW): over three times that of the control cultures. qPCR analyses showed that in SA-treated cells, the expression of the genes coding for phenylalanine ammonia-lyase (PAL), cinnamoyl-CoA reductase (CCR) and cinnamyl-alcohol dehydrogenase (CAD), all involved in the first steps of PTOX biosynthesis, also increased reaching a peak 8-12 h after the treatment. Expression of the pinoresinol-lariciresinol reductase gene (PLR), which is involved in one of the last biosynthetic steps, was not affected by SA. The selective action of SA on these genes can be applied to control the biotechnological production of this anticancer agent.

show abstract

“…Other processes have been described based on the rehydration of powdered tissues of Po. However, a new optimized method has recently been developed for PTOX extraction from cell cultures, which are a promising source of this bioactive compound [44]. This allows endogenous b-glucosidases to hydrolyze lignans [42].…”

Section: Extraction and Synthesismentioning

confidence: 99%

“…hexandrum is listed in the appendix II of CITES (Convention for International Trade in Endangered Species) [43]. However, a new optimized method has recently been developed for PTOX extraction from cell cultures, which are a promising source of this bioactive compound [44].…”

Section: Ptox Sources 51 Extraction and Synthesismentioning

confidence: 99%

Podophyllotoxin: Current approaches to its biotechnological production and future challenges

Yousefzadi¹,

Sharifi²,

Behmanesh³

et al. 2010

Engineering in Life Sciences

Self Cite

View full text Add to dashboard Cite

Many plant‐derived agents are being used to treat cancer, including taxol, vinblastine, vincristine, or camptothecin and podophyllotoxin derivatives, among others. Plant biotechnology can provide a new tool for the production of anticancer agents but in spite of considerable efforts to produce vinblastine and vincristine in cell cultures and knowledge of the biosynthetic pathway of Catharanthus roseus alkaloids, the biotechnological production of taxol has only been achieved at an industrial level by companies such as Phyton Biotech and Cytoclonal Pharmaceutics. Podophyllotoxin was isolated as the active antitumor agent from the roots of Podophyllum species and more recently from the genus Linum and others. Etoposide, teniposide, and etophos are semi‐synthetic derivatives of podophyllotoxin and are used in the treatment of cancer. Biotechnological approaches, including the use of cell cultures, biotransformation, or metabolic engineering techniques to manipulate the biosynthetic pathway, represent an alternative for the production of podophyllotoxin and are discussed in this review.

show abstract

Optimization of podophyllotoxin extraction method fromLinum albumcell cultures

Cited by 29 publications

References 14 publications

Cereal Lignans, Natural Compounds of Interest for Human Health?

Cereal Lignans, Natural Compounds of Interest for Human Health?

Salicylic acid improves podophyllotoxin production in cell cultures of Linum album by increasing the expression of genes related with its biosynthesis

Podophyllotoxin: Current approaches to its biotechnological production and future challenges

Contact Info

Product

Resources

About