Comparison of desiccation-induced gene expression in vegetative tissue and seed of the resurrection plant, Xerophyta humilis

Walford, Sally-Ann; Denby, Katherine J.; Shen, Arthur; Farrant, Jill M.; Illing, Nicola

doi:10.1016/j.sajb.2007.04.034

Cited by 3 publications

(4 citation statements)

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“…, 2002; Mulako et al. , 2008; Walford et al. , 2007), thereby supporting the hypothesis that DT in vegetative tissue is a consequence of the activation of seed‐specific genes in roots and leaves upon dehydration, these being induced in response to environmental rather than developmental cues (Illing et al.…”

Section: Discussionmentioning

confidence: 73%

“…We have used differential screening and microarray technology to identify genes upregulated during drying in angiosperm vegetative tissues of the resurrection plants Xerophyta viscosa and Xerophyta humilis (Collett et al, 2004;Mundree and Farrant, 2000), and are in the process of inserting such genes into crops for improved drought stress Garwe et al, 2003;Iyer et al, 2007). Furthermore, we have recently found considerable similarity in genes induced during the acquisition of DT in seeds, and in vegetative tissues of X. humilis Mulako et al, 2008;Walford et al, 2007), thereby supporting the hypothesis that DT in vegetative tissue is a consequence of the activation of seed-specific genes in roots and leaves upon dehydration, these being induced in response to environmental rather than developmental cues (Illing et al, 2005). An understanding of the environmental signals that induce DT in vegetative tissue, and the implementation of them in crops, could facilitate the induction of drought-tolerant crops without genetic modification.…”

Section: Discussionmentioning

confidence: 99%

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Desiccation tolerance in the vegetative tissues of the fern Mohria caffrorum is seasonally regulated

Farrant¹,

Lehner

Cooper

et al. 2008

The Plant Journal

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SummaryAs there is limited information on the mechanisms of vegetative desiccation tolerance in pteridophytes, we undertook a comprehensive anatomical, ultrastructural, physiological and biochemical study on the fern Mohria caffrorum. Our data show that this species is desiccation-tolerant during the dry season, and desiccation-sensitive in the rainy season. This system allows the verification of protection mechanisms by comparison of tolerant and sensitive tissues of the same species at the same developmental age. Tolerant fronds acquire protection mechanisms during drying that are mostly similar to those reported for angiosperms. These include: (i) chlorophyll masking by abaxial scales and frond curling; (ii) increased antioxidant capacity that is maintained in dry tissues; (iii) mechanical stabilization of vacuoles in the dry state; (iv) de novo production of heat stable proteins (at least one identified as a putative chaperonin); (v) accummulation of protective carbohydrates (sucrose, raffinose family oligosaccharides and cyclitols). This study has implications for the biotechnological production of drought-tolerant crops, and allows speculation on the evolution of vegetative desiccation tolerance.

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Section: Discussionmentioning

confidence: 73%

Section: Discussionmentioning

confidence: 99%

Desiccation tolerance in the vegetative tissues of the fern Mohria caffrorum is seasonally regulated

Farrant¹,

Lehner

Cooper

et al. 2008

The Plant Journal

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“…Fundamental discoveries of the tolerance strategies utilised by resurrection plants include the early detection of dehydration and shut-down of photosynthesis, the presence of extensive ROS scavenging systems, even in the hydrated state, the accumulation of sugars, as well as the enrichment of transcripts associated with cell wall plasticity[ 3 – 12 ]. Importantly, transcripts and metabolites typically associated with gene profiles observed in seeds are often detected within vegetative tissues, leading to the hypothesis that resurrection plants conform to a dormant “seed-like” state upon drying [ 3 , 4 , 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

Trehalose Accumulation Triggers Autophagy during Plant Desiccation

et al. 2015

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Global climate change, increasingly erratic weather and a burgeoning global population are significant threats to the sustainability of future crop production. There is an urgent need for the development of robust measures that enable crops to withstand the uncertainty of climate change whilst still producing maximum yields. Resurrection plants possess the unique ability to withstand desiccation for prolonged periods, can be restored upon watering and represent great potential for the development of stress tolerant crops. Here, we describe the remarkable stress characteristics of Tripogon loliiformis, an uncharacterised resurrection grass and close relative of the economically important cereals, rice, sorghum, and maize. We show that T. loliiformis survives extreme environmental stress by implementing autophagy to prevent Programmed Cell Death. Notably, we identified a novel role for trehalose in the regulation of autophagy in T.loliiformis. Transcriptome, Gas Chromatography Mass Spectrometry, immunoblotting and confocal microscopy analyses directly linked the accumulation of trehalose with the onset of autophagy in dehydrating and desiccated T. loliiformis shoots. These results were supported in vitro with the observation of autophagosomes in trehalose treated T. loliiformis leaves; autophagosomes were not detected in untreated samples. Presumably, once induced, autophagy promotes desiccation tolerance in T.loliiformis, by removal of cellular toxins to suppress programmed cell death and the recycling of nutrients to delay the onset of senescence. These findings illustrate how resurrection plants manipulate sugar metabolism to promote desiccation tolerance and may provide candidate genes that are potentially useful for the development of stress tolerant crops.

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“…Desiccation sensitive Arabidopsis thaliana cannot survive exposure to a water deficit equivalent to less than 85-95% (v/v) relative humidity during the vegetative growth period, although desiccation tolerance is an integral part of the normal seed development program in higher plants ( Bartels and Salamini 2001;Illing et al 2005;Ingle et al 2007a;Vicre et al 2004;Walford et al 2007). Desiccation sensitive plants utilize the protective mechanisms to retain water and limit the amount of stress-associated dehydration damage to a level that can be sustained for a short period of time but eventually die as these mechanisms are overwhelmed by stress damage (Oliver 2007).…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of nuclear proteins during dehydration of the resurrection plant Xerophyta viscosa

2010

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Xerophyta viscosa (family Velloziaceae) is a monocotyledonous poikilochlorophyllous desiccation tolerant plant capable of surviving drying its leaf tissue to 5% relative water content (RWC) for prolonged periods and resuming full physiological activity upon rehydration. X. viscosa and other resurrection plants are excellent model systems for the study of desiccation tolerance mechanisms. In this work, the X. viscosa nuclear protein profile was investigated in response to dehydration stress using 2D gel electrophoresis, protein map analysis and mass spectrometry identification. From an average of 438 protein spots detected on the gels, 18 spots were significantly shown to increase in abundance upon dehydration at 35% RWC. Analysis of the 18 dehydration-induced nuclear protein spots resulted in the identification of proteins associated with gene transcription and regulation, cell signalling, molecular chaperone and proteolysis type activities, protein translation, energy metabolism, and novel proteins. This study revealed that X. viscosa nucleus responded to dehydration stress and that desiccation tolerance was controlled by multiple genes within the plant nucleus. This study indicated that adjusting gene regulation and expression of appropriate stress response proteins in the nucleus was sufficient to protect the cellular structures during dehydration and in the dried state.

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Comparison of desiccation-induced gene expression in vegetative tissue and seed of the resurrection plant, Xerophyta humilis

Cited by 3 publications

References 0 publications

Desiccation tolerance in the vegetative tissues of the fern Mohria caffrorum is seasonally regulated

Desiccation tolerance in the vegetative tissues of the fern Mohria caffrorum is seasonally regulated

Trehalose Accumulation Triggers Autophagy during Plant Desiccation

Proteomic analysis of nuclear proteins during dehydration of the resurrection plant Xerophyta viscosa

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