The purpose of this study was to obtain reference values of serum chemistry in Korean rockfish (Sebastes schlegeli), 5 juvenile (n=25) and 3 broodstock (n=15) subpopulations which were differentiated by cultured method. Serum samples were analyzed for the following commercial clinical kits: total protein, albumin, glucose, AST (aspartate aminotransferase), ALT (alanine aminotransferase), total cholesterol, free cholesterol, LDH (lactate dehydrogenase) and ALP (alkaline phosphatase). Significant differences were observed in the values of total protein, albumin, total cholesterol, AST, ALT, LDH and ALP between juvenile and broodstock subpopulations. In the comparison of total protein, albumin, total cholesterol and ALP, broodstock subpopulations showed higher levels. On the other hand, increased AST, ALT and LDH were observed in juvenile subpopulations. These results indicate that cultured method are major contributors to health-related physiology of Korean rockfish, juveniles and broodstocks.