Environmental stress-related gene expression and blood physiological responses in olive flounder (<i>Paralichthys olivaceus</i>) exposed to osmotic and thermal stress

Choi, Cheol Young

doi:10.1080/19768351003764940

Cited by 18 publications

(5 citation statements)

References 34 publications

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“…7B). This protein is thought to be involved in the warm temperature acclimatory response (Kikuchi et al, 1995) and the heat stress response in fish (Picard and Schulte, 2004;Pierre et al, 2010;Choi, 2010). In contrast to previous studies, WAP-65 increased with 9°C acclimation in G. mirabilis, suggesting that this protein may play a role in thermal acclimation regardless of the temperature and that its role might vary depending on the organism.…”

Section: Iron Homeostasis Antioxidant Response and Hypoxic Injurycontrasting

confidence: 52%

Proteomic analysis of cardiac response to thermal acclimation in the eurythermal goby fishGillichthys mirabilis

Jayasundara

Tomanek

Dowd

et al. 2015

Journal of Experimental Biology

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Cardiac function is thought to play a central role in determining thermal optima and tolerance limits in teleost fishes. Investigating proteomic responses to temperature in cardiac tissues may provide insights into mechanisms supporting the thermal plasticity of cardiac function. Here, we utilized a global proteomic analysis to investigate changes in cardiac protein abundance in response to temperature acclimation (transfer from 13°C to 9, 19 and 26°C) in a eurythermal goby, Gillichthys mirabilis. Proteomic data revealed 122 differentially expressed proteins across acclimation groups, 37 of which were identified using tandem mass-spectrometry. These 37 proteins are involved in energy metabolism, mitochondrial regulation, iron homeostasis, cytoprotection against hypoxia, and cytoskeletal organization. Compared with the 9 and 26°C groups, proteins involved in energy metabolism increased in 19°C-acclimated fish, indicating an overall increase in the capacity for ATP production. Creatine kinase abundance increased in 9°C-acclimated fish, suggesting an important role for the phosphocreatine energy shuttle in cold-acclimated hearts. Both 9 and 26°C fish also increased abundance of hexosaminidase, a protein directly involved in posthypoxia stress cytoprotection of cardiac tissues. Cytoskeletal restructuring appears to occur in all acclimation groups; however, the most prominent effect was detected in 26°C-acclimated fish, which exhibited significantly increased actin levels. Overall, proteomic analysis of cardiac tissue suggests that the capacity to adjust ATP-generating processes is crucial to the thermal plasticity of cardiac function. Furthermore, G. mirabilis may optimize cellular functions at temperatures near 19°C, which lies within the species' preferred temperature range.

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Section: Iron Homeostasis Antioxidant Response and Hypoxic Injurycontrasting

confidence: 52%

Proteomic analysis of cardiac response to thermal acclimation in the eurythermal goby fishGillichthys mirabilis

Jayasundara

Tomanek

Dowd

et al. 2015

Journal of Experimental Biology

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“…While we found HSP 70, which is a universal indicator of the stress response, to be differentially expressed, fold changes observed were modest (+1.68) and much lower compared to published data assessing stress effects on fish such as salinity, oxygen and temperature (Fig. 4 and SI Table 8), where fold changes ranged from 2.5 to 372 FC 43–45 . Interestingly, it seems that the reaction in tissues of adult fish generally results in much higher fold changes, e.g.…”

Section: Discussioncontrasting

confidence: 84%

Transcriptome profiling reveals exposure to predicted end-of-century ocean acidification as a stealth stressor for Atlantic cod larvae

Mittermayer

Stiasny

Clemmesen

et al. 2019

Sci Rep

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Ocean acidification (OA), a direct consequence of increasing atmospheric CO2 concentration dissolving in ocean waters, is impacting many fish species. Little is known about the molecular mechanisms underlying the observed physiological impacts in fish. We used RNAseq to characterize the transcriptome of 3 different larval stages of Atlantic cod (Gadus morhua) exposed to simulated OA at levels (1179 µatm CO2) representing end-of-century predictions compared to controls (503 µatm CO2), which were shown to induce tissue damage and elevated mortality in G. morhua. Only few genes were differentially expressed in 6 and 13 days-post-hatching (dph) (3 and 16 genes, respectively), during a period when maximal mortality as a response to elevated pCO2 occurred. At 36 dph, 1413 genes were differentially expressed, most likely caused by developmental asynchrony between the treatment groups, with individuals under OA growing faster. A target gene analysis revealed only few genes of the universal and well-defined cellular stress response to be differentially expressed. We thus suggest that predicted ocean acidification levels constitute a “stealth stress” for early Atlantic cod larvae, with a rapid breakdown of cellular homeostasis leading to organismal death that was missed even with an 8-fold replication implemented in this study.

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“…Nowadays, rising marine temperatures have influenced the survival, growth, distribution, and reproduction of this species [ 56 , 57 , 58 ]. Numerous studies have illustrated the effect of heat or cold stress on the masculinization, sex-manipulation, energy metabolism, neurosecretion, stress-related gene expression and AMPK regulation mechanism of Japanese flounder [ 54 , 55 , 57 , 59 , 60 ]. As a critical functional organ in fish, the gill is involved in a number of physiological processes, including respiration, waste nitrogen excretion, iono-/osmoregulation, immune response and acid–base balance [ 61 , 62 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Mechanism Based on Histopathology, Antioxidant System and Transcriptomic Profiles in Heat Stress Response in the Gills of Japanese Flounder

Yan

Qiao

et al. 2022

IJMS

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As an economically important flatfish in Asia, Japanese flounder is threatened by continuously rising temperatures due to global warming. To understand the molecular responses of this species to temperature stress, adult Japanese flounder individuals were treated with two kinds of heat stress—a gradual temperature rise (GTR) and an abrupt temperature rise (ATR)—in aquaria under experimental conditions. Changes in histopathology, programmed cell death levels and the oxidative stress status of gills were investigated. Histopathology showed that the damage caused by ATR stress was more serious. TUNEL signals confirmed this result, showing more programmed cell death in the ATR group. In addition, reactive oxygen species (ROS) levels and the 8-O-hDG contents of both the GTR and ATR groups increased significantly, and the total superoxide dismutase (T-SOD) activities and total antioxidant capacity (T-AOC) levels decreased in the two stressed groups, which showed damage to antioxidant systems. Meanwhile, RNA-seq was utilized to illustrate the molecular mechanisms underyling gill damage. Compared to the control group of 18 °C, 507 differentially expressed genes (DEGs) were screened in the GTR group; 341 were up-regulated and 166 were down-regulated, and pathway enrichment analysis indicated that they were involved in regulation and adaptation, including chaperone and folding catalyst pathways, the mitogen-activated protein kinase signaling (MAPK) pathway and DNA replication protein pathways. After ATR stress, 1070 DEGs were identified, 627 were up-regulated and 423 were down-regulated, and most DEGs were involved in chaperone and folding catalyst and DNA-related pathways, such as DNA replication proteins and nucleotide excision repair. The annotation of DEGs showed the great importance of heat shock proteins (HSPs) in protecting Japanese flounder from heat stress injury; 12 hsp genes were found after GTR, while 5 hsp genes were found after ATR. In summary, our study records gill dysfunction after heat stress, with different response patterns observed in the two experimental designs; chaperones were activated to defend heat stress after GTR, while replication was almost abandoned due to the severe damage consequent on ATR stress.

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Environmental stress-related gene expression and blood physiological responses in olive flounder (Paralichthys olivaceus) exposed to osmotic and thermal stress

Cited by 18 publications

References 34 publications

Proteomic analysis of cardiac response to thermal acclimation in the eurythermal goby fishGillichthys mirabilis

Proteomic analysis of cardiac response to thermal acclimation in the eurythermal goby fishGillichthys mirabilis

Transcriptome profiling reveals exposure to predicted end-of-century ocean acidification as a stealth stressor for Atlantic cod larvae

Molecular Mechanism Based on Histopathology, Antioxidant System and Transcriptomic Profiles in Heat Stress Response in the Gills of Japanese Flounder

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