Comparison of Antioxidant and Anti-hyaluronidase Potentials of Pineapple Core Extract (Ananas comosus (L.) Merr.) and Luteolin

Abstract

Skin aging processes are divided into chronological aging and premature aging. Premature aging is generally caused by free radicals, from both air pollution and photoaging. Natural compounds from plant extracts are among sources of antioxidants and anti-hyaluronidase which have the ability to prevent antiaging. One of the potential fruits related to antioxidant and antiaging activities is Anana scomosus. A. comosus has a number of phenolic compounds with biological activities. One of the main phenolic compounds in A. comosus is luteolin. The aim of this study was to evaluate the antioxidant and antiaging potentials of pineapple core extract (PCE). This study was conducted at the Biomolecular and Biomedicine Research Center, Aretha Medika Utama from August to November 2018. Analysis of antioxidants from PCE and luteolin was carried out using H 2 O 2 scavenging activity assay. The antiaging assay was carried out through inhibition of hyaluronidase enzyme, one of the important enzymes in the aging process. Luteolin had lower IC50 value of H 2 O 2 scavenging activity of around 24.12±3.13 μg/ ml, which was better than CPE with IC50 of 304.56±3.76μg/mL. The results of hyaluronidase inhibition activity assay showed that luteolin compound had a lower IC50 value of 67.38±3.99 μg/mL when compared to PCE with an IC50 value of 161.15±1.05 μg/mL. Hence, Luteolin has higher antioxidant and anti-hyaluronidase activities than PCE

Potentials of rose (Rosa damascena) petals and receptacles extract as antioxidant and antihyaluronidase

“…The H 2 O 2 presence in the reaction will cause the complex not to be formed. So if there is an antioxidant that traps H 2 O 2 , then the mixture color is orange that show the presence of Fe 2+ -tri-phenanthroline complex (Stevenie et al, 2019;Asan et al, 2019;Jusri et al, 2019;Liana et al, 2019;Utami et al, 2017).…”

“…Turbidity that can be read starting from 540 nm is proportional to the concentration of HA. The enzyme inhibitory activity determined based on the HA higher concentration remaining after the reaction is stopped (Stevenie et al, 2019;Asan et al, 2019;Jusri et al, 2019;Liana et al, 2019;Utami et al, 2017 In the anti-hyaluronidase activity test, the final concentration of the sample used was 166.67 µg/mL; 83.33 µg/mL; 41.67 µg/mL; 20.83 µg/mL; 10.42 µg/mL; and 5.22 µg/mL. The results of antihyaluronidase activities from RPE and RRE can be seen in Figure 4, while IC 50 values can be seen in Table 2.…”

“…Then it was incubated again in the same condition for 10 mins. The absorbances were determined at 510 nm (Asan et al, 2019;Jusri et al, 2019;Liana et al, 2019;Utami et al, 2017). The scavenging activities percentage of samples were calculated using this following formula : H 2 O 2 Scavenging Activity (%) = (Ac -As)/Ac × 100 Ac: control absorbance As: sample absorbance…”

Antioxidant and antiaging activity of rutin and caffeic acid

“…The method employed by Asan et al (2019), Jusri et al (2019), Liana et al (2019), and Utami et al (2017) to measure H2O2 scavenging activities was used with modifications. Ferrous ammonium sulfate (1 mM, 12 µl) and samples at various concentrations (7.81-250 µg/ml, 60 µl), H2O2 (5 mM, 3 µl) were transferred into a 96-well plate and incubated at room temperature for 5 min.…”

“…This activity was estimated using a method that had been previously employed by Asan et al (2019), Jusri et al (2019), Liana et al, (2019) and Widowati et al (2016Widowati et al ( , 2017Widowati et al ( , 2018. A mixture containing samples (0.78-50 µg/mL, 25 µl), phosphate buffer (12 µl), and the enzyme hyaluronidase (3 µl) was incubated at 37 °C for 10 min.…”