Comparison of anaerobic digestion strategies of nitrogen-rich substrates: Performance of anaerobic reactors and microbial community diversity

Ziganshina, Elvira E.; Ibragimov, Emil M.; Vankov, Petr Y.; Miluykov, Vasili; Ziganshin, Ayrat M.

doi:10.1016/j.wasman.2016.10.038

Cited by 53 publications

(31 citation statements)

References 54 publications

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“…A. oremlandii also oxidizes acetate, with reduction of thiosulfate as the electron acceptor (Fisher et al ., ), which is a capability it shares with the SAOB T. phaeum (Hattori et al ., ). Alkaliphilus species have also been detected in high‐ammonia biogas digesters (> 0.3 g NH 3 ‐N l −1 /8–10 g NH + 4 ‐N l −1 , Kovács et al ., ; Müller et al ., ; Tsapekos et al ., ; Ziganshina et al ., ), suggesting that they play a critical role in biogas digesters operating under such conditions. Their presence has been attributed to the ability of certain members to encode crucial peptidases for proteolysis of proteins (Stolze et al ., ).…”

Section: Discussionmentioning

confidence: 99%

Detection of novel syntrophic acetate‐oxidizing bacteria from biogas processes by continuous acetate enrichment approaches

Westerholm

Müller

Singh

et al. 2017

Microbial Biotechnology

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SummaryTo enrich syntrophic acetate‐oxidizing bacteria (SAOB), duplicate chemostats were inoculated with sludge from syntrophic acetate oxidation (SAO)‐dominated systems and continuously supplied with acetate (0.4 or 7.5 g l−1) at high‐ammonia levels. The chemostats were operated under mesophilic (37°C) or thermophilic (52°C) temperature for about six hydraulic retention times (HRT 28 days) and were sampled over time. Irrespective of temperature, a methane content of 64–69% and effluent acetate level of 0.4–1.0 g l−1 were recorded in chemostats fed high acetate. Low methane production in the low‐acetate chemostats indicated that the substrate supply was below the threshold for methanization of acetate via SAO. Novel representatives within the family Clostridiales and genus Syntrophaceticus (class Clostridia) were identified to represent putative SAOB candidates in mesophilic and thermophilic conditions respectively. Known SAOB persisted at low relative abundance in all chemostats. The hydrogenotrophic methanogens Methanoculleus bourgensis (mesophilic) and Methanothermobacter thermautotrophicus (thermophilic) dominated archaeal communities in the high‐acetate chemostats. In line with the restricted methane production in the low‐acetate chemostats, methanogens persisted at considerably lower abundance in these chemostats. These findings strongly indicate involvement in SAO and tolerance to high ammonia levels of the species identified here, and have implications for understanding community function in stressed anaerobic processes.

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Section: Discussionmentioning

confidence: 99%

Detection of novel syntrophic acetate‐oxidizing bacteria from biogas processes by continuous acetate enrichment approaches

Westerholm

Müller

Singh

et al. 2017

Microbial Biotechnology

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“…accumulated during the reactor operation at increasing OLR (up to 2.84 g VS L −1 day −1 ; Table S1, SI). However, the prevalence of Methanosarcina in reactors utilizing chicken manure as the sole substrate and operating at high TAN/FAN levels was also reported [46, 47], indicating that substrate differences and the inoculum used to start the process might have a crucial impact on the development of microorganisms. In addition, reactor R3.1 operated at high levels of VFA (primarily of acetate and propionate, up to 9.9 g L −1 and 4.1 g L −1 , accordingly).…”

Section: Discussionmentioning

confidence: 99%

“…Methanosarcina spp. are found in various environments [56] and reported to achieve stable growth at high OLR and high levels of ammonium in biogas reactors, indicating their tolerance towards different process impairments [12], and therefore they are frequently found in various biogas reactors (e.g., [8, 14, 47, 55, 58]).…”

Section: Discussionmentioning

confidence: 99%

Comparative Analysis of Methanogenic Communities in Different Laboratory-Scale Anaerobic Digesters

Ziganshin

Ziganshina

Kleinsteuber

et al. 2016

Archaea

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Comparative analysis of methanogenic archaea compositions and dynamics in 11 laboratory-scale continuous stirred tank reactors fed with different agricultural materials (chicken manure, cattle manure, maize straw, maize silage, distillers grains, and Jatropha press cake) was carried out by analysis of the methyl coenzyme-M reductase α-subunit (mcrA) gene. Various taxa within Methanomicrobiales, Methanobacteriaceae, Methanosarcinaceae, Methanosaetaceae, and Methanomassiliicoccales were detected in the biogas reactors but in different proportions depending on the substrate type utilized as well as various process parameters. Improved coverage and higher taxonomic resolution of methanogens were obtained compared to a previous 16S rRNA gene based study of the same reactors. Some members of the genus Methanoculleus positively correlated with the relative methane content, whereas opposite correlations were found for Methanobacterium. Specific biogas production was found to be significantly correlating with Methanosarcinaceae. Statistical analysis also disclosed that some members of the genus Methanoculleus positively correlated with the ammonia level, whereas the prevalence of Methanocorpusculum, Methanobacterium, and Methanosaeta was negatively correlated with this parameter. These results suggest that the application of methanogenic archaea adapted to specific feedstock might enhance the anaerobic digestion of such waste materials in full-scale biogas reactors.

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“…End-products from glucose fermentation were acetate, lactate, ethanol, CO 2 , and H 2 [43]. Caldicoprobacter was also detected from continuous stirred tank reactors digesting chicken manure at mesophilic temperature [44]. Ruminofilibacter, Mobilitalea, uncultured (family Bacteroidales UCG-001) and uncultured (family Cloacimonetes-W5) for treatment groups were extreme significant (p < 0.01) higher than control groups.…”

Section: Microbial Community At Genus Levelmentioning

confidence: 93%

Long-Term Mesophilic Anaerobic Co-Digestion of Swine Manure with Corn Stover and Microbial Community Analysis

et al. 2020

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Long-term anaerobic co-digestion of swine manure (SM) and corn stover (CS) was conducted using semi-continuously loaded digesters under mesophilic conditions. A preliminary test was first conducted to test the effects of loading rates, and results indicated the 3 g-VS L−1 d−1 was the optimal loading rate. Based on the preliminary results, a verification replicated test was conducted with 3 g-VS L−1 d−1 loading rate and different SM/CS ratios (1:1, 2:1 and 1:2). Results showed that a SM/CS ratio of 2/1 was optimal, based on maximum observed methane-VSdes generation and carbon conversion efficiency (72.56 ± 3.40 mL g−1 and 40.59%, respectively). Amplicon sequencing analysis suggested that microbial diversity was increased with CS loading. Amino-acid-degrading bacteria were abundant in the treatment groups. Archaea Methanoculleus could enhance biogas and methane productions.

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Comparison of anaerobic digestion strategies of nitrogen-rich substrates: Performance of anaerobic reactors and microbial community diversity

Cited by 53 publications

References 54 publications

Detection of novel syntrophic acetate‐oxidizing bacteria from biogas processes by continuous acetate enrichment approaches

Detection of novel syntrophic acetate‐oxidizing bacteria from biogas processes by continuous acetate enrichment approaches

Comparative Analysis of Methanogenic Communities in Different Laboratory-Scale Anaerobic Digesters

Long-Term Mesophilic Anaerobic Co-Digestion of Swine Manure with Corn Stover and Microbial Community Analysis

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