Detection of novel syntrophic acetate‐oxidizing bacteria from biogas processes by continuous acetate enrichment approaches

Westerholm, Maria; Müller, Bettina; Singh, Abhijeet; Lindsjö, Oskar Karlsson; Schnürer, Anna

doi:10.1111/1751-7915.13035

Cited by 59 publications

(34 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The FTHFS gene was amplified with previously developed primers ( 21 ), with some modifications ( 54 ). For the restriction reactions, the amplified FTHFS gene fragment of ∼635 bp was purified using the E-Gel Safe Imager system and E-Gel SizeSelect 2% agarose gels (Invitrogen).…”

Section: Methodsmentioning

confidence: 99%

Effect of Start-Up Strategies and Electrode Materials on Carbon Dioxide Reduction on Biocathodes

Saheb-Alam

Singh

Hermansson

et al. 2018

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

The enrichment of CO2-reducing microbial biocathodes is challenging. Previous research has shown that a promising approach could be to first enrich bioanodes and then lower the potential so the electrodes are converted into biocathodes. However, the effect of such a transition on the microbial community on the electrode has not been studied. The goal of this study was thus to compare the start-up of biocathodes from preenriched anodes with direct start-up from bare electrodes and to investigate changes in microbial community composition. The effect of three electrode materials on the long-term performance of the biocathodes was also investigated. In this study, preenrichment of acetate-oxidizing bioanodes did not facilitate the start-up of biocathodes. It took about 170 days for the preenriched electrodes to generate substantial cathodic current, compared to 83 days for the bare electrodes. Graphite foil and carbon felt cathodes produced higher current at the beginning of the experiment than did graphite rods. However, all electrodes produced similar current densities at the end of the over 1-year-long study (2.5 A/m2). Methane was the only product detected during operation of the biocathodes. Acetate was the only product detected after inhibition of the methanogens. Microbial community analysis showed that Geobacter sp. dominated the bioanodes. On the biocathodes, the Geobacter sp. was succeeded by Methanobacterium spp., which made up more than 80% of the population. After inhibition of the methanogens, Acetobacterium sp. became dominant on the electrodes (40% relative abundance). The results suggested that bioelectrochemically generated H2 acted as an electron donor for CO2 reduction.IMPORTANCE In microbial electrochemical systems, living microorganisms function as catalysts for reactions on the anode and/or the cathode. There is a variety of potential applications, ranging from wastewater treatment and biogas generation to production of chemicals. Systems with biocathodes could be used to reduce CO2 to methane, acetate, or other high-value chemicals. The technique can be used to convert solar energy to chemicals. However, enriching biocathodes that are capable of CO2 reduction is more difficult and less studied than enriching bioanodes. The effect of different start-up strategies and electrode materials on the microbial communities that are enriched on biocathodes has not been studied. The purpose of this study was to investigate two different start-up strategies and three different electrode materials for start-up and long-term operation of biocathodes capable of reducing CO2 to valuable biochemicals.

show abstract

Section: Methodsmentioning

confidence: 99%

Effect of Start-Up Strategies and Electrode Materials on Carbon Dioxide Reduction on Biocathodes

Saheb-Alam

Singh

Hermansson

et al. 2018

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…The known SAOB that could be detected were Syntrophaceticus and Tepidanerobacter, both indicators for the pH 7.0-8.5 cluster and members of the order Thermoanaerobacterales [93,106]. Although the only cultivated member of the genus Syntrophaceticus (S. schinkii) is mesophilic, 16S rRNA genes of related species have been detected in various thermophilic sludge communities [93,107]. The thermotolerant species Tepidanerobacter acetatoxidans was isolated from a mesophilic methanogenic system and is able to perform both acidogenesis and SAO [106].…”

Section: Effect Of Ph On Physiochemical and Microbiological Aspects Omentioning

confidence: 99%

pH and Phosphate Induced Shifts in Carbon Flow and Microbial Community during Thermophilic Anaerobic Digestion

et al. 2020

View full text Add to dashboard Cite

pH is a central environmental factor influencing CH4 production from organic substrates, as every member of the complex microbial community has specific pH requirements. Here, we show how varying pH conditions (5.0–8.5, phosphate buffered) and the application of a phosphate buffer per se induce shifts in the microbial community composition and the carbon flow during nine weeks of thermophilic batch digestion. Beside monitoring the methane production as well as volatile fatty acid concentrations, amplicon sequencing of the 16S rRNA gene was conducted. The presence of 100 mM phosphate resulted in reduced CH4 production during the initial phase of the incubation, which was characterized by a shift in the dominant methanogenic genera from a mixed Methanosarcina and Methanoculleus to a pure Methanoculleus system. In buffered samples, acetate strongly accumulated in the beginning of the batch digestion and subsequently served as a substrate for methanogens. Methanogenesis was permanently inhibited at pH values ≤5.5, with the maximum CH4 production occurring at pH 7.5. Adaptations of the microbial community to the pH variations included shifts in the archaeal and bacterial composition, as less competitive organisms with a broad pH range were able to occupy metabolic niches at unfavorable pH conditions.

show abstract

“…Hence, it is suggested that no H 2 limitation was prevalent within the analyzed thermophilic biogas fermenter and/or M. wolfeii is syntrophically connected with H 2 supplying bacteria. Syntrophic interactions and especially the syntrophic hydrogen transfer between Bacteria and hydrogenotrophic Archaea are often described in the context of the biogas process [74][75][76]. Syntrophic growth of M. thermautotrophicus with Syntrophothermus lipocaldicus revealed, that the methyl-coenzyme M reductase I (Mcr) was preferred instead of methyl-coenzyme M reductase II (Mrt), in contrast to expression of both enzymes in pure culture of M. thermautotrophicus [77].…”

Section: Reconstruction and Transcriptional Activity Of The Hydrogenomentioning

confidence: 99%

Genome Analyses and Genome-Centered Metatranscriptomics of Methanothermobacter wolfeii Strain SIV6, Isolated from a Thermophilic Production-Scale Biogas Fermenter

et al. 2019

View full text Add to dashboard Cite

In the thermophilic biogas-producing microbial community, the genus Methanothermobacter was previously described to be frequently abundant. The aim of this study was to establish and analyze the genome sequence of the archaeal strain Methanothermobacter wolfeii SIV6 originating from a thermophilic industrial-scale biogas fermenter and compare it to related reference genomes. The circular chromosome has a size of 1,686,891 bases, featuring a GC content of 48.89%. Comparative analyses considering three completely sequenced Methanothermobacter strains revealed a core genome of 1494 coding sequences and 16 strain specific genes for M. wolfeii SIV6, which include glycosyltransferases and CRISPR/cas associated genes. Moreover, M. wolfeii SIV6 harbors all genes for the hydrogenotrophic methanogenesis pathway and genome-centered metatranscriptomics indicates the high metabolic activity of this strain, with 25.18% of all transcripts per million (TPM) belong to the hydrogenotrophic methanogenesis pathway and 18.02% of these TPM exclusively belonging to the mcr operon. This operon encodes the different subunits of the enzyme methyl-coenzyme M reductase (EC: 2.8.4.1), which catalyzes the final and rate-limiting step during methanogenesis. Finally, fragment recruitment of metagenomic reads from the thermophilic biogas fermenter on the SIV6 genome showed that the strain is abundant (1.2%) within the indigenous microbial community. Detailed analysis of the archaeal isolate M. wolfeii SIV6 indicates its role and function within the microbial community of the thermophilic biogas fermenter, towards a better understanding of the biogas production process and a microbial-based management of this complex process.

show abstract

Detection of novel syntrophic acetate‐oxidizing bacteria from biogas processes by continuous acetate enrichment approaches

Cited by 59 publications

References 64 publications

Effect of Start-Up Strategies and Electrode Materials on Carbon Dioxide Reduction on Biocathodes

Effect of Start-Up Strategies and Electrode Materials on Carbon Dioxide Reduction on Biocathodes

pH and Phosphate Induced Shifts in Carbon Flow and Microbial Community during Thermophilic Anaerobic Digestion

Genome Analyses and Genome-Centered Metatranscriptomics of Methanothermobacter wolfeii Strain SIV6, Isolated from a Thermophilic Production-Scale Biogas Fermenter

Contact Info

Product

Resources

About