Comparative Analysis of Methanogenic Communities in Different Laboratory-Scale Anaerobic Digesters

Abstract

Comparative analysis of methanogenic archaea compositions and dynamics in 11 laboratory-scale continuous stirred tank reactors fed with different agricultural materials (chicken manure, cattle manure, maize straw, maize silage, distillers grains, and Jatropha press cake) was carried out by analysis of the methyl coenzyme-M reductase α-subunit (mcrA) gene. Various taxa within Methanomicrobiales, Methanobacteriaceae, Methanosarcinaceae, Methanosaetaceae, and Methanomassiliicoccales were detected in the biogas reactors but in different proportions depending on the substrate type utilized as well as various process parameters. Improved coverage and higher taxonomic resolution of methanogens were obtained compared to a previous 16S rRNA gene based study of the same reactors. Some members of the genus Methanoculleus positively correlated with the relative methane content, whereas opposite correlations were found for Methanobacterium. Specific biogas production was found to be significantly correlating with Methanosarcinaceae. Statistical analysis also disclosed that some members of the genus Methanoculleus positively correlated with the ammonia level, whereas the prevalence of Methanocorpusculum, Methanobacterium, and Methanosaeta was negatively correlated with this parameter. These results suggest that the application of methanogenic archaea adapted to specific feedstock might enhance the anaerobic digestion of such waste materials in full-scale biogas reactors.

Unravelling diversity and metabolic potential of microbial consortia at each stage of leather sewage treatment

“…4b), however, they disappeared in active populations and new genera including Methanosarcina and Methanosaeta, occurred, despite their low relative abundance in the 16S rRNA amplicon reads, which most likely be explained by the fact that the number of ribosome were much higher in active cells than that in dormant cells. 43 Previous studies demonstrated that Methanosaeta, a methanogenic archaea exclusively conducting acetoclastic methanogenesis, was negatively correlated with the total ammonium nitrogen concentration and kept increased activity under high organic acids concentrations, 44,45 which was in line with the increase in the relative abundance of Methanosaeta and the decline of total ammonium nitrogen concentration in the present study ( Fig. 4b, Table 1).…”

Assessment of multiple anaerobic co-digestions and related microbial community of molasses with rice alcohol wastewater

“…Methanosaetaceae, the methanogens can survive at both high and low concentrations of acetate [57,58]. In both the mono-digestion and co-digestion process, Methanobacteriaceae was found in all stages and ranged from 12.02-20.44%, which is in the line with the work of Gagliano et al [59] and Ziganshin et al [60]. Methanobacteriaceae is the primary user of formate and H2/CO2 substrates for methane formation.…”

Effects of graphene oxide on the performance, microbial community dynamics and antibiotic resistance genes reduction during anaerobic digestion of swine manure

“…The changes of intI1 representing the mobile genetic elements (MGEs) was followed to reflect the potential of HGT and multi-resistance (Engelstädter et al, 2016). The functional gene mcrA involved the methane production was quantified to reflect the effects of GO addition on the methanogenesis (Ziganshin et al, 2016).…”