Comparison of an array of in vitro assays for the assessment of the estrogenic potential of natural and synthetic estrogens, phytoestrogens and xenoestrogens

Gutendorf, Brigitte; Westendorf, Johannes

doi:10.1016/s0300-483x(01)00437-1

Cited by 384 publications

(229 citation statements)

References 24 publications

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“…E1 was about 15 times less potent, NP about 1 × 10 5 times less potent and OP about 3 × 10 5 times less potent than E2. The observed relative estrogenic potencies of E2, E1, NP and OP are in agreement with reference data (Gutendrof et al, 2001;Legler et al, 2002;Van den Belt et al, 2004). These chemicals were previously analyzed in another in vitro bioassay using a recombinant yeast cell culture.…”

Section: Estrogenic Activity Of Selected Chemicalssupporting

confidence: 87%

“…An interesting observation is the estrogenic activity of BPA in the yeast culture assay which had a relative potency of 1.1 × 10 − 4 and was the most potent in Rutishshauser et al's (2004) study among NP, OP and BPA. In contrast, Gutendrof et al (2001) reported a relative potency of 2.5 × 10 − 5 in the MVLN-cells assay for BPA, but in this study, BPA showed weak estrogenic activity at a high concentration of 1 × 10 5 nM. As a result, the relative estrogenic potencies of selected chemicals in this study descended in the order of E2 N E1 N NP N OP N BPA.…”

Section: Estrogenic Activity Of Selected Chemicalscontrasting

confidence: 64%

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Measurement of estrogenic activity in sediments from Haihe and Dagu River, China

Song

Jiang

et al. 2006

Environment International

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Sediments from two rivers in China, the Haihe and Dagu Rivers, were examined for estrogenic activity using an estrogen receptor (ER)-mediated in vitro bioassay system. ER-active compounds were isolated from sediments by Soxhlet extraction, and the crude extracts were fractionated using a florisil column into three fractions. The estrogenic activity of each extract was detected by measuring luciferase activity in the human breast cancer cell line MCF-7 transfected with a luciferase receptor gene. Significant estrogenic activity was observed in each total extract. The 17β-estradiol equivalents (E2-EQs) ranged from 8.24 to 95.28 ng E2 g − 1 dw. As a result, the relative estrogenic potencies of three fractions in this study descended in the order of Fraction 3 N Fraction 2 N Fraction 1. The results of the bioassay analysis indicated the heavy pollution status of these sites with estrogenic contaminants. In this study, five selected chemicals, the natural estrogens 17β-estradiol (E2) and estrone (E1), and the xeno-estrogens 4-octylphenol (OP), 4-nonylphenol (NP), and Bisphenol A (BPA) were also analyzed using the in vitro bioassay. The estrogenic activity of these chemicals were E2 N E1 N NP N OP N BPA.

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Section: Estrogenic Activity Of Selected Chemicalssupporting

confidence: 87%

Section: Estrogenic Activity Of Selected Chemicalscontrasting

confidence: 64%

Measurement of estrogenic activity in sediments from Haihe and Dagu River, China

Song

Jiang

et al. 2006

Environment International

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“…Mechanistic experiments indicate that these chemicals interact with the estrogen receptors (ERs) and stimulate or inhibit some sensitive cell types in a number of different ways (Ferguson et al, 2000;Gutendorf and Westendorf, 2001;Holmes et al, 2004). There are also many compounds which exhibit other effects, including antiestrogenic, androgenic or antiandrogenic.…”

Section: Introductionmentioning

confidence: 99%

Female sexual maturation and reproduction after prepubertal exposure to estrogens and endocrine disrupting chemicals: A review of rodent and human data

Rasier

Toppari

Parent

et al. 2006

Molecular and Cellular Endocrinology

144

108

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Natural hormones and some synthetic chemicals spread into our surrounding environment share the capacity to interact with hormone action and metabolism. Exposure to such compounds can cause a variety of developmental and reproductive detrimental abnormalities in wildlife species and, potentially, in human. Many experimental and epidemiological data have reported that exposure of the developing fetus or neonate to environmentally relevant concentrations of some among these endocrine disrupters induces morphological, biochemical and/or physiological disorders in brain and reproductive organs, by interfering with the hormone actions. The impact of such exposures on the hypothalamic-pituitary-gonadal axis and subsequent sexual maturation is the subject of the present review. We will highlight epidemiological human studies and the effects of early exposure during gestational, perinatal or postnatal life in female rodents.

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“…[2][3][4] Soybean isoflavones function as estrogenic agents in estrogen-deficient conditions, and they conversely function as anti-estrogenic agents or have no effect in estrogen-sufficient conditions. 5) Increases in 17β-estradiol suppress food intake, and consequently induce decreases in body weight gain.…”

mentioning

confidence: 99%

Dynamics of appetite-mediated gene expression in daidzein-fed female rats in the meal-feeding method

Fujitani

Maekawa

Bhattarai

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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We previously found that daidzein decreased food intake in female rats. The present study aimed to elucidate the relationship between dynamics of appetite-mediated neuropeptides and the anorectic effect of daidzein. We examined appetite-mediated gene expression in the hypothalamus and small intestine during the 3 meals per day feeding method. Daidzein had an anorectic effect specifically at the second feeding. Neuropeptide-Y (NPY) and galanin mRNA levels in the hypothalamus were significantly higher after feeding in the control but not in the daidzein group, suggesting that daidzein attenuated the postprandial increase in NPY and galanin expression. The daidzein group had higher corticotrophin-releasing hormone (CRH) mRNA levels in the hypothalamus after feeding, and increased cholelcystokinin (CCK) mRNA levels in the small intestine, suggesting that CCK is involved in the hypothalamic regulation of this　anorectic effect. Therefore, daidzein may induce anorexia by suppressing expression of NPY and galanin and increasing expression of CRH in the hypothalamus.

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Comparison of an array of in vitro assays for the assessment of the estrogenic potential of natural and synthetic estrogens, phytoestrogens and xenoestrogens

Cited by 384 publications

References 24 publications

Measurement of estrogenic activity in sediments from Haihe and Dagu River, China

Measurement of estrogenic activity in sediments from Haihe and Dagu River, China

Female sexual maturation and reproduction after prepubertal exposure to estrogens and endocrine disrupting chemicals: A review of rodent and human data

Dynamics of appetite-mediated gene expression in daidzein-fed female rats in the meal-feeding method

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