Comparison between the growth pattern of cell cultures from normal and Duchenne dystrophy muscle

Delaporte, C; Dehaupas, Michèle; Fardeau, Michel

doi:10.1016/0022-510x(84)90033-9

Cited by 71 publications

(55 citation statements)

References 13 publications

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“…Analysis of the expression levels of lnc-31 in mdx animals, where intense regeneration occurs through myoblast activation (37), and in human DMD myoblasts, which have a delayed differentiation phenotype (18,26,44), further supports the notion that lnc-31 inversely correlates with the ability of myogenic cells to progress into the differentiation program.…”

Section: Figmentioning

confidence: 66%

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Novel Long Noncoding RNAs (lncRNAs) in Myogenesis: a miR-31 Overlapping lncRNA Transcript Controls Myoblast Differentiation

Ballarino

Cazzella

D’Andrea

et al. 2015

Molecular and Cellular Biology

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Transcriptome analysis allowed the identification of new long noncoding RNAs differentially expressed during murine myoblast differentiation. These transcripts were classified on the basis of their expression under proliferating versus differentiated conditions, muscle-restricted activation, and subcellular localization. Several species displayed preferential expression in dystrophic (mdx) versus wild-type muscles, indicating their possible link with regenerative processes. One of the identified transcripts, lnc-31, even if originating from the same nuclear precursor of miR-31, is produced by a pathway mutually exclusive. We show that lnc-31 and its human homologue hsa-lnc-31 are expressed in proliferating myoblasts, where they counteract differentiation. In line with this, both species are more abundant in mdx muscles and in human Duchenne muscular dystrophy (DMD) myoblasts, than in their normal counterparts. Altogether, these data suggest a crucial role for lnc-31 in controlling the differentiation commitment of precursor myoblasts and indicate that its function is maintained in evolution despite the poor sequence conservation with the human counterpart.

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Section: Figmentioning

confidence: 66%

“…S4B). In contrast, in human Duchenne (DMD) myoblasts, characterized by a delay in the differentiation program (18,26,44), lnc-31 downregulation is less pronounced (see Fig. S4B).…”

Section: Figmentioning

confidence: 98%

Novel Long Noncoding RNAs (lncRNAs) in Myogenesis: a miR-31 Overlapping lncRNA Transcript Controls Myoblast Differentiation

Ballarino

Cazzella

D’Andrea

et al. 2015

Molecular and Cellular Biology

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“…Although human DMD mpc have reduced proliferative capacity (Blau et al 1983;Melone et al 1999), they can differentiate in vitro into myotubes and normal mpc (Blau et al 1983), but only when contaminating fibroblasts have been removed from the preparation (Delaporte et al 1984). The mpc from mdx EDL muscles have been reported to differentiate to the same extent as mpc from age-matched control animals, but mpc from some mdx mice displayed poor differentiation (Schuierer et al 2005).…”

Section: Satellite Cells In Pathological Conditionsmentioning

confidence: 99%

Are Human and Mouse Satellite Cells Really the Same?

Boldrin¹,

Muntoni²,

Morgan³

2010

J Histochem Cytochem.

116

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S U M M A R Y Satellite cells are quiescent cells located under the basal lamina of skeletal muscle fibers that contribute to muscle growth, maintenance, repair, and regeneration. Mouse satellite cells have been shown to be muscle stem cells that are able to regenerate muscle fibers and self-renew. As human skeletal muscle is also able to regenerate following injury, we assume that the human satellite cell is, like its murine equivalent, a muscle stem cell. In this review, we compare human and mouse satellite cells and highlight their similarities and differences. We discuss gaps in our knowledge of human satellite cells, compared with that of mouse satellite cells, and suggest ways in which we may advance studies on human satellite cells, particularly by finding new markers and attempting to re-create the human satellite cell niche in vitro. (J Histochem Cytochem 58:941-955, 2010)

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“…SM was isolated after biopsy of the skeletal muscle of the lower limb, according to the technique of Delaporte [11]. In brief, the skeletal tissue was digested with 0.075% type II collagenase (Sigma, US) in a 5% CO 2 incubator for 1 h at 37°C.…”

Section: Mscs and Sm Isolation And Coculturementioning

confidence: 99%

Cardiac Analysis of Autologous Transplantation of Cocultured Skeletal Myoblasts and Mesenchymal Cells in a Rat Model Doxorubicin-Induced Cardiotoxicity: Histopathological and Functional Studies

Dias¹,

Francisco²,

Cardoso³

2015

J Clin Exp Cardiolog

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Objective: Investigate the functional and histopathological effects of combined transplantation of mesenchymal stem cells (MSCs) and skeletal myoblasts (SM) in rats submitted to doxorubicin (DOX) induced cardiomyopathy. Methods:Myocardiopathy was induced through intraperitoneal applications of 3.75 mg/kg/day DOX once a week for 4 weeks in all experimental animals. Echocardiography examination was conducted to evaluate heart function. Myocardial cell apoptosis was examined morphologically by optical microscopy. The expression of both, von Willebrand factor and MyoD proteins was analysed using immunohistochemical staining. Results:Our results showed that all the experimental animals developed cardio-toxicity and exhibited decrease in heart function 4 weeks after the administration of DOX (P<0.05). The ventricular function significantly improved in rats that received sub-epicardial injection of co-culture of SM and MSC (CO group) when compared to rats that received only saline sub-epicardial injection (CG group) (P<0.05). Conclusion:The results of this study provide evidences that the myocardial co-culture transplantation of SM and MSCs contributed to the treatment the DOX-induced cardiotoxicity.

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Comparison between the growth pattern of cell cultures from normal and Duchenne dystrophy muscle

Cited by 71 publications

References 13 publications

Novel Long Noncoding RNAs (lncRNAs) in Myogenesis: a miR-31 Overlapping lncRNA Transcript Controls Myoblast Differentiation

Novel Long Noncoding RNAs (lncRNAs) in Myogenesis: a miR-31 Overlapping lncRNA Transcript Controls Myoblast Differentiation

Are Human and Mouse Satellite Cells Really the Same?

Cardiac Analysis of Autologous Transplantation of Cocultured Skeletal Myoblasts and Mesenchymal Cells in a Rat Model Doxorubicin-Induced Cardiotoxicity: Histopathological and Functional Studies

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